Human T-cell leukemia virus type 2 Rex inhibits pre-mRNA splicing in vitro at an early stage of spliceosome formation

Andreas Bakker, Xinqiang Li, Cristina T. Ruland, Dennis W. Stephens, Alexander C. Black, Joseph D. Rosenblatt

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

The Rex protein is an essential regulator of RNA expression in human T- cell leukemia virus types 1 and 2 (HTLV-1 and HTLV-2) that promotes the accumulation of full-length and partially spliced viral transcripts in the cytoplasm. Rex-mediated regulation correlates with specific binding to a cognate RNA recognition element which overlaps the 5' splice site in the viral long terminal repeat. It has been unclear whether Rex directly affects splicing or only nuclear-to-cytoplasmic transport of viral mRNA. We demonstrate that HTLV-2 Rex is a potent inhibitor of splicing in vitro at an early step in spliceosome assembly. Inhibition requires phosphorylation of Rex and the ability of Rex to bind to the Rex response element. Direct inhibition of early spliceosome assembly by Rex may account for differential accumulation of unspliced transcripts and represents a novel mechanism of retroviral gene regulation.

Original languageEnglish (US)
Pages (from-to)5511-5518
Number of pages8
JournalJournal of virology
Volume70
Issue number8
DOIs
StatePublished - Aug 1996

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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