TY - JOUR
T1 - Human RFP2 gene promoter
T2 - Unique structure and unusual strength
AU - Skoblov, Mikhail
AU - Shakhbazov, Konstantin
AU - Oshchepkov, Dmitry
AU - Ivanov, Dmitry
AU - Guskova, Anna
AU - Ivanov, Dmitry
AU - Rubtsov, Petr
AU - Prasolov, Vladimir
AU - Yankovsky, Nick
AU - Baranova, Ancha
PY - 2006/4/14
Y1 - 2006/4/14
N2 - Human gene RFP2 is a candidate tumor suppressor located at 13q14.3 and deleted in multiple tumor types. To explore regulation of RFP2, we determined structure of the 5′-untranslated region of RFP2 gene and its promoter. RFP2 promoter area is TATA-less, highly enriched in G and C nucleotides, and contains multiple quadruplex forming GGGGA-repeats. Deletion analysis of 5′-flanking sequences demonstrated that repeat containing fragment possesses activity seven times exceeding that of the combined SV40 promoter/enhancer. Other unusual features of the RFP2 promoter include anomalously high electrostatic fields induced by sequence-dependent dipoles and very low nucleosome forming potential. A "minimized" version of the RFP2 promoter could be used for overexpression of the various transgenes in the mammalian cells.
AB - Human gene RFP2 is a candidate tumor suppressor located at 13q14.3 and deleted in multiple tumor types. To explore regulation of RFP2, we determined structure of the 5′-untranslated region of RFP2 gene and its promoter. RFP2 promoter area is TATA-less, highly enriched in G and C nucleotides, and contains multiple quadruplex forming GGGGA-repeats. Deletion analysis of 5′-flanking sequences demonstrated that repeat containing fragment possesses activity seven times exceeding that of the combined SV40 promoter/enhancer. Other unusual features of the RFP2 promoter include anomalously high electrostatic fields induced by sequence-dependent dipoles and very low nucleosome forming potential. A "minimized" version of the RFP2 promoter could be used for overexpression of the various transgenes in the mammalian cells.
KW - Candidate tumor suppressor gene RFP2
KW - Chronic lymphocytic leukemia
KW - Luciferase assay
KW - Nucleosome forming potential
KW - Promoter
KW - Quadruplex
UR - http://www.scopus.com/inward/record.url?scp=33644612705&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33644612705&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2006.01.187
DO - 10.1016/j.bbrc.2006.01.187
M3 - Article
C2 - 16499869
AN - SCOPUS:33644612705
VL - 342
SP - 859
EP - 866
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -