Human RFP2 gene promoter: Unique structure and unusual strength

Mikhail Skoblov; Konstantin Shakhbazov; Dmitry Oshchepkov; Dmitry Ivanov; Anna Guskova; Dmitry Ivanov; Petr Rubtsov; Vladimir Prasolov; Nick Yankovsky; Ancha Baranova

doi:10.1016/j.bbrc.2006.01.187

Human RFP2 gene promoter: Unique structure and unusual strength

Mikhail Skoblov, Konstantin Shakhbazov, Dmitry Oshchepkov, Dmitry Ivanov, Anna Guskova, Dmitry Ivanov, Petr Rubtsov, Vladimir Prasolov, Nick Yankovsky, Ancha Baranova

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Human gene RFP2 is a candidate tumor suppressor located at 13q14.3 and deleted in multiple tumor types. To explore regulation of RFP2, we determined structure of the 5′-untranslated region of RFP2 gene and its promoter. RFP2 promoter area is TATA-less, highly enriched in G and C nucleotides, and contains multiple quadruplex forming GGGGA-repeats. Deletion analysis of 5′-flanking sequences demonstrated that repeat containing fragment possesses activity seven times exceeding that of the combined SV40 promoter/enhancer. Other unusual features of the RFP2 promoter include anomalously high electrostatic fields induced by sequence-dependent dipoles and very low nucleosome forming potential. A "minimized" version of the RFP2 promoter could be used for overexpression of the various transgenes in the mammalian cells.

Original language	English (US)
Pages (from-to)	859-866
Number of pages	8
Journal	Biochemical and biophysical research communications
Volume	342
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2006.01.187
State	Published - Apr 14 2006
Externally published	Yes

Keywords

Candidate tumor suppressor gene RFP2
Chronic lymphocytic leukemia
Luciferase assay
Nucleosome forming potential
Promoter
Quadruplex

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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Human RFP2 gene promoter : Unique structure and unusual strength. / Skoblov, Mikhail; Shakhbazov, Konstantin; Oshchepkov, Dmitry; Ivanov, Dmitry; Guskova, Anna; Ivanov, Dmitry; Rubtsov, Petr; Prasolov, Vladimir; Yankovsky, Nick; Baranova, Ancha.

In: Biochemical and biophysical research communications, Vol. 342, No. 3, 14.04.2006, p. 859-866.

Research output: Contribution to journal › Article › peer-review

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AU - Shakhbazov, Konstantin

AU - Oshchepkov, Dmitry

AU - Ivanov, Dmitry

AU - Guskova, Anna

AU - Ivanov, Dmitry

AU - Rubtsov, Petr

AU - Prasolov, Vladimir

AU - Yankovsky, Nick

AU - Baranova, Ancha

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AB - Human gene RFP2 is a candidate tumor suppressor located at 13q14.3 and deleted in multiple tumor types. To explore regulation of RFP2, we determined structure of the 5′-untranslated region of RFP2 gene and its promoter. RFP2 promoter area is TATA-less, highly enriched in G and C nucleotides, and contains multiple quadruplex forming GGGGA-repeats. Deletion analysis of 5′-flanking sequences demonstrated that repeat containing fragment possesses activity seven times exceeding that of the combined SV40 promoter/enhancer. Other unusual features of the RFP2 promoter include anomalously high electrostatic fields induced by sequence-dependent dipoles and very low nucleosome forming potential. A "minimized" version of the RFP2 promoter could be used for overexpression of the various transgenes in the mammalian cells.

KW - Candidate tumor suppressor gene RFP2

KW - Chronic lymphocytic leukemia

KW - Luciferase assay

KW - Nucleosome forming potential

KW - Promoter

KW - Quadruplex

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Human RFP2 gene promoter: Unique structure and unusual strength

Abstract

Keywords

ASJC Scopus subject areas

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