Human RFP2 gene promoter: Unique structure and unusual strength

Mikhail Skoblov, Konstantin Shakhbazov, Dmitry Oshchepkov, Dmitry Ivanov, Anna Guskova, Dmitry Ivanov, Petr Rubtsov, Vladimir Prasolov, Nick Yankovsky, Ancha Baranova

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Human gene RFP2 is a candidate tumor suppressor located at 13q14.3 and deleted in multiple tumor types. To explore regulation of RFP2, we determined structure of the 5′-untranslated region of RFP2 gene and its promoter. RFP2 promoter area is TATA-less, highly enriched in G and C nucleotides, and contains multiple quadruplex forming GGGGA-repeats. Deletion analysis of 5′-flanking sequences demonstrated that repeat containing fragment possesses activity seven times exceeding that of the combined SV40 promoter/enhancer. Other unusual features of the RFP2 promoter include anomalously high electrostatic fields induced by sequence-dependent dipoles and very low nucleosome forming potential. A "minimized" version of the RFP2 promoter could be used for overexpression of the various transgenes in the mammalian cells.

Original languageEnglish (US)
Pages (from-to)859-866
Number of pages8
JournalBiochemical and biophysical research communications
Volume342
Issue number3
DOIs
StatePublished - Apr 14 2006

Keywords

  • Candidate tumor suppressor gene RFP2
  • Chronic lymphocytic leukemia
  • Luciferase assay
  • Nucleosome forming potential
  • Promoter
  • Quadruplex

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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  • Cite this

    Skoblov, M., Shakhbazov, K., Oshchepkov, D., Ivanov, D., Guskova, A., Ivanov, D., Rubtsov, P., Prasolov, V., Yankovsky, N., & Baranova, A. (2006). Human RFP2 gene promoter: Unique structure and unusual strength. Biochemical and biophysical research communications, 342(3), 859-866. https://doi.org/10.1016/j.bbrc.2006.01.187