Human protectin (CD59), an 18-20-kD homologous complement restriction factor, does not restrict perforin-mediated lysis

Seppo Meri, B. Paul Morgan, Mark Wing, Jane Jones, Alexandra Davies, Eckhard Podack, Peter J. Lachmann

Research output: Contribution to journal › Article

38 Citations (Scopus)

Abstract

Human protectin (CD59) is an 18-20-kD membrane glycoprotein that restricts lysis of human erythrocytes and leukocytes by homologous complement. By directly incorporating protectin into membranes of heterologous cells we observed that protectin did not prevent perforin-mediated killing, whereas complement killing was effectively restricted. Further, no significant enhancement of cell-mediated killing or target killing by purified perforin was observed with anti-protectin antibodies. Thus, in contrast with complement lysis, restriction of lysis by protectin does not apply to cell-mediated killing.

Original languageEnglish
Pages (from-to)367-370
Number of pages4
JournalJournal of Experimental Medicine
Volume172
Issue number1
StatePublished - Jul 1 1990
Externally publishedYes

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CD59 Antigens
Perforin
Membrane Glycoproteins
Anti-Idiotypic Antibodies
Leukocytes
Erythrocytes
Cell Membrane

ASJC Scopus subject areas

  • Immunology

Cite this

Meri, S., Morgan, B. P., Wing, M., Jones, J., Davies, A., Podack, E., & Lachmann, P. J. (1990). Human protectin (CD59), an 18-20-kD homologous complement restriction factor, does not restrict perforin-mediated lysis. Journal of Experimental Medicine, 172(1), 367-370.

Human protectin (CD59), an 18-20-kD homologous complement restriction factor, does not restrict perforin-mediated lysis. / Meri, Seppo; Morgan, B. Paul; Wing, Mark; Jones, Jane; Davies, Alexandra; Podack, Eckhard; Lachmann, Peter J.

In: Journal of Experimental Medicine, Vol. 172, No. 1, 01.07.1990, p. 367-370.

Research output: Contribution to journal › Article

Meri, S, Morgan, BP, Wing, M, Jones, J, Davies, A, Podack, E & Lachmann, PJ 1990, 'Human protectin (CD59), an 18-20-kD homologous complement restriction factor, does not restrict perforin-mediated lysis', Journal of Experimental Medicine, vol. 172, no. 1, pp. 367-370.
Meri S, Morgan BP, Wing M, Jones J, Davies A, Podack E et al. Human protectin (CD59), an 18-20-kD homologous complement restriction factor, does not restrict perforin-mediated lysis. Journal of Experimental Medicine. 1990 Jul 1;172(1):367-370.
Meri, Seppo ; Morgan, B. Paul ; Wing, Mark ; Jones, Jane ; Davies, Alexandra ; Podack, Eckhard ; Lachmann, Peter J. / Human protectin (CD59), an 18-20-kD homologous complement restriction factor, does not restrict perforin-mediated lysis. In: Journal of Experimental Medicine. 1990 ; Vol. 172, No. 1. pp. 367-370.
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