Human papillomavirus DNA in adenocarcinoma in situ, microinvasive adenocarcinoma of the uterine cervix, and coexisting cervical squamous intraepithelial neoplasia

T. Tase, T. Okagaki, B. A. Clark, L. B. Twiggs, R. S. Ostrow, A. J. Faras

Research output: Contribution to journalArticle

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Abstract

Previously, human papillomavirus (HPV) DNA, mainly HPV-18 DNA, was detected in more than 40% (17/40 cases) of invasive adenocarcinoma of the uterine cervix in our laboratory. In order to identify HPV DNA in the precursor lesions of adenocarcinoma of the cervix, 11 cases of adenocarcinoma in situ containing microinvasive adenocarcinoma and 10 cases of adenocarcinoma in situ were studied for the presence of HPV DNA by in situ hybridization using highly sensitive 3H-labeled HPV-16 and HPV-18 DNA probes. HPV types present in cervical squamous intraepithelial neoplasia (CIN) coexisting with adenocarcinoma in situ and microinvasive adenocarcinoma were also studied. Apart from the coexisting CIN II-III with glandular neoplasms, 48 cases of CIN III (severe dysplasia and squamous carcinoma in situ) removed by conization or hysterectomy and known to be free of adenocarcinoma were used for comparison. HPV DNA was detected in 64% of microinvasive adenocarcinoma, 70% of adenocarcinoma in situ, and 63% of the control CIN III. HPV-18 DNA was the preponderant type of HPV DNA found in adenocarcinoma in situ and microinvasive adenocarcinoma. All cases of HPV DNA-positive microinvasive adenocarcinoma contained the same type of HPV DNA as the lesions of coexisting adenocarcinoma in situ. CIN coexisting with microinvasive adenocarcinoma or adenocarcinoma in situ contained the same type of HPV as identified in the glandular lesions, whereas all of the HPV DNA-positive control CIN III cases contained HPV-16 DNA. These results suggest that adenocarcinoma in situ is a precursor lesion of adenocarcinoma of the cervix that contains HPV DNA, and that CIN coexisting with adenocarcinoma may be a result of a metaplastic process of adenocarcinoma or of bidirectional differentiation of the affected reserve cells.

Original languageEnglish
Pages (from-to)8-17
Number of pages10
JournalInternational Journal of Gynecological Pathology
Volume8
Issue number1
StatePublished - Jan 1 1989
Externally publishedYes

Fingerprint

Cervical Intraepithelial Neoplasia
Cervix Uteri
Adenocarcinoma
DNA
Human papillomavirus 18
Human papillomavirus 16
Adenocarcinoma in Situ
Conization
Glandular and Epithelial Neoplasms
Carcinoma in Situ
DNA Probes
Hysterectomy
In Situ Hybridization
Squamous Cell Carcinoma

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Pathology and Forensic Medicine

Cite this

Human papillomavirus DNA in adenocarcinoma in situ, microinvasive adenocarcinoma of the uterine cervix, and coexisting cervical squamous intraepithelial neoplasia. / Tase, T.; Okagaki, T.; Clark, B. A.; Twiggs, L. B.; Ostrow, R. S.; Faras, A. J.

In: International Journal of Gynecological Pathology, Vol. 8, No. 1, 01.01.1989, p. 8-17.

Research output: Contribution to journalArticle

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