Human pancreatic carcinoma (mia paca‐2) in continuous culture: Sensitivity to asparaginase

Adel A. Yunis, Grace K. Arimura, David J. Russin

Research output: Contribution to journalArticlepeer-review

265 Scopus citations


An undifferentiated human pancreatic carcinoma has been established in continuous culture and is grown in Dulbecco's modified Eagle's medium fortified with 10% fetal calf serum and 2.5% horse serum. The established cell line (MIA PaCa‐2) has a doulbing time of 40 h. The cells are large with abundant cytoplasm, exhibit a high degree of aneuploidy and have a tendency to grow on top of other cells. MIA PaCa‐2 grows in soft agar with a colony‐forming efficiency of 19%. Both MIA PaCa‐2 cells and a cell line from another pancreatic carcinoma obtained from National Cancer Institute (NCI) are sensitive to asparaginase, a property not shared by several other human tumor cell lines tested.

Original languageEnglish (US)
Pages (from-to)128-135
Number of pages8
JournalInternational Journal of Cancer
Issue number1
StatePublished - Jan 15 1977

ASJC Scopus subject areas

  • Oncology
  • Cancer Research


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