Human pancreatic carcinoma (mia paca‐2) in continuous culture: Sensitivity to asparaginase

Adel A. Yunis; Grace K. Arimura; David J. Russin

doi:10.1002/ijc.2910190118

Human pancreatic carcinoma (mia paca‐2) in continuous culture: Sensitivity to asparaginase

Adel A. Yunis, Grace K. Arimura, David J. Russin

Medicine

Research output: Contribution to journal › Article › peer-review

265 Scopus citations

Abstract

An undifferentiated human pancreatic carcinoma has been established in continuous culture and is grown in Dulbecco's modified Eagle's medium fortified with 10% fetal calf serum and 2.5% horse serum. The established cell line (MIA PaCa‐2) has a doulbing time of 40 h. The cells are large with abundant cytoplasm, exhibit a high degree of aneuploidy and have a tendency to grow on top of other cells. MIA PaCa‐2 grows in soft agar with a colony‐forming efficiency of 19%. Both MIA PaCa‐2 cells and a cell line from another pancreatic carcinoma obtained from National Cancer Institute (NCI) are sensitive to asparaginase, a property not shared by several other human tumor cell lines tested.

Original language	English (US)
Pages (from-to)	128-135
Number of pages	8
Journal	International Journal of Cancer
Volume	19
Issue number	1
DOIs	https://doi.org/10.1002/ijc.2910190118
State	Published - Jan 15 1977

ASJC Scopus subject areas

Oncology
Cancer Research

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abstract = "An undifferentiated human pancreatic carcinoma has been established in continuous culture and is grown in Dulbecco's modified Eagle's medium fortified with 10% fetal calf serum and 2.5% horse serum. The established cell line (MIA PaCa‐2) has a doulbing time of 40 h. The cells are large with abundant cytoplasm, exhibit a high degree of aneuploidy and have a tendency to grow on top of other cells. MIA PaCa‐2 grows in soft agar with a colony‐forming efficiency of 19%. Both MIA PaCa‐2 cells and a cell line from another pancreatic carcinoma obtained from National Cancer Institute (NCI) are sensitive to asparaginase, a property not shared by several other human tumor cell lines tested.",

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AB - An undifferentiated human pancreatic carcinoma has been established in continuous culture and is grown in Dulbecco's modified Eagle's medium fortified with 10% fetal calf serum and 2.5% horse serum. The established cell line (MIA PaCa‐2) has a doulbing time of 40 h. The cells are large with abundant cytoplasm, exhibit a high degree of aneuploidy and have a tendency to grow on top of other cells. MIA PaCa‐2 grows in soft agar with a colony‐forming efficiency of 19%. Both MIA PaCa‐2 cells and a cell line from another pancreatic carcinoma obtained from National Cancer Institute (NCI) are sensitive to asparaginase, a property not shared by several other human tumor cell lines tested.

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Human pancreatic carcinoma (mia paca‐2) in continuous culture: Sensitivity to asparaginase

Abstract

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