Human pancreatic carcinoma (MIA PaCa 2) in continuous culture: sensitivity to asparaginase

Adel A Yunis, G. K. Arimura, D. J. Russin

Research output: Contribution to journalArticle

252 Citations (Scopus)

Abstract

An undifferentiated human pancreatic carcinoma has been established in continuous culture and is grown in Dulbecco's modified Eagle's medium fortified with 10% fetal calf serum and 2.5% horse serum. The established cell line (MIA PaCa 2) has a doubling time of 40 h. The cells are large with abundant cytoplasm, exhibit a high degree of aneuploidy and have a tendency to grow on top of other cells. MIA PaCa 2 grows in soft agar with a colony forming efficiency of 19%. Both MIA PaCa 2 cells and a cell line from another pancreatic carcinoma obtained from National Cancer Institute (NCI) are sensitive to asparaginase, a property not shared by several other human tumor cell lines tested.

Original languageEnglish (US)
Pages (from-to)128-135
Number of pages8
JournalInternational Journal of Cancer
Volume19
Issue number1
StatePublished - 1977

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Asparaginase
Cell Line
Eagles
National Cancer Institute (U.S.)
Aneuploidy
Tumor Cell Line
Serum
Horses
Agar
Cytoplasm
Pancreatic Carcinoma

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Human pancreatic carcinoma (MIA PaCa 2) in continuous culture : sensitivity to asparaginase. / Yunis, Adel A; Arimura, G. K.; Russin, D. J.

In: International Journal of Cancer, Vol. 19, No. 1, 1977, p. 128-135.

Research output: Contribution to journalArticle

Yunis, Adel A ; Arimura, G. K. ; Russin, D. J. / Human pancreatic carcinoma (MIA PaCa 2) in continuous culture : sensitivity to asparaginase. In: International Journal of Cancer. 1977 ; Vol. 19, No. 1. pp. 128-135.
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