Human pancreatic carcinoma (MIA PaCa-2) in continuous culture: sensitivity to asparaginase.

A. A. Yunis; G. K. Arimura; D. J. Russin

Human pancreatic carcinoma (MIA PaCa-2) in continuous culture: sensitivity to asparaginase.

A. A. Yunis, G. K. Arimura, D. J. Russin

Medicine

Research output: Contribution to journal › Article › peer-review

54 Scopus citations

Abstract

An undifferentiated human pancreatic carcinoma has been established in continuous culture and is grown in Dulbecco's modified. Eagle's medium fortified with 10% fetal calf serum and 2.5% horse serum. The established cell line (MIA PaCa-2) has a doubling time of 40 h. The cells are large with abundant cytoplasm, exhibit a high degree of aneuploidy and have a tendency to grow on top of other cells. MIA PaCa-2 grows in soft agar with a colony-forming efficiency of 19%. Both MIA PaCa-2 cells and a cell line from another pancreatic carcinoma obtained from National Cancer Institute (NCI) are sensitive to asparaginase, a property not shared by several other human tumor cell lines tested.

Original language	English (US)
Pages (from-to)	218-235
Number of pages	18
Journal	International Journal of Cancer
Volume	19
Issue number	1
State	Published - Jan 1 1977

ASJC Scopus subject areas

Oncology
Cancer Research

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abstract = "An undifferentiated human pancreatic carcinoma has been established in continuous culture and is grown in Dulbecco's modified. Eagle's medium fortified with 10% fetal calf serum and 2.5% horse serum. The established cell line (MIA PaCa-2) has a doubling time of 40 h. The cells are large with abundant cytoplasm, exhibit a high degree of aneuploidy and have a tendency to grow on top of other cells. MIA PaCa-2 grows in soft agar with a colony-forming efficiency of 19%. Both MIA PaCa-2 cells and a cell line from another pancreatic carcinoma obtained from National Cancer Institute (NCI) are sensitive to asparaginase, a property not shared by several other human tumor cell lines tested.",

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Human pancreatic carcinoma (MIA PaCa-2) in continuous culture: sensitivity to asparaginase.

Abstract

ASJC Scopus subject areas

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