Abstract
An undifferentiated human pancreatic carcinoma has been established in continuous culture and is grown in Dulbecco's modified. Eagle's medium fortified with 10% fetal calf serum and 2.5% horse serum. The established cell line (MIA PaCa-2) has a doubling time of 40 h. The cells are large with abundant cytoplasm, exhibit a high degree of aneuploidy and have a tendency to grow on top of other cells. MIA PaCa-2 grows in soft agar with a colony-forming efficiency of 19%. Both MIA PaCa-2 cells and a cell line from another pancreatic carcinoma obtained from National Cancer Institute (NCI) are sensitive to asparaginase, a property not shared by several other human tumor cell lines tested.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 218-235 |
| Number of pages | 18 |
| Journal | International Journal of Cancer |
| Volume | 19 |
| Issue number | 1 |
| State | Published - Jan 1 1977 |
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ASJC Scopus subject areas
- Oncology
- Cancer Research
Cite this
Yunis, A. A., Arimura, G. K., & Russin, D. J. (1977). Human pancreatic carcinoma (MIA PaCa-2) in continuous culture: sensitivity to asparaginase. International Journal of Cancer, 19(1), 218-235.