Human pancreatic carcinoma (MIA PaCa-2) in continuous culture: sensitivity to asparaginase.

A. A. Yunis, G. K. Arimura, D. J. Russin

Research output: Contribution to journalArticlepeer-review

54 Scopus citations


An undifferentiated human pancreatic carcinoma has been established in continuous culture and is grown in Dulbecco's modified. Eagle's medium fortified with 10% fetal calf serum and 2.5% horse serum. The established cell line (MIA PaCa-2) has a doubling time of 40 h. The cells are large with abundant cytoplasm, exhibit a high degree of aneuploidy and have a tendency to grow on top of other cells. MIA PaCa-2 grows in soft agar with a colony-forming efficiency of 19%. Both MIA PaCa-2 cells and a cell line from another pancreatic carcinoma obtained from National Cancer Institute (NCI) are sensitive to asparaginase, a property not shared by several other human tumor cell lines tested.

Original languageEnglish (US)
Pages (from-to)218-235
Number of pages18
JournalInternational Journal of Cancer
Issue number1
StatePublished - Jan 1 1977

ASJC Scopus subject areas

  • Oncology
  • Cancer Research


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