Abstract
An undifferentiated human pancreatic carcinoma has been established in continuous culture and is grown in Dulbecco's modified. Eagle's medium fortified with 10% fetal calf serum and 2.5% horse serum. The established cell line (MIA PaCa-2) has a doubling time of 40 h. The cells are large with abundant cytoplasm, exhibit a high degree of aneuploidy and have a tendency to grow on top of other cells. MIA PaCa-2 grows in soft agar with a colony-forming efficiency of 19%. Both MIA PaCa-2 cells and a cell line from another pancreatic carcinoma obtained from National Cancer Institute (NCI) are sensitive to asparaginase, a property not shared by several other human tumor cell lines tested.
Original language | English (US) |
---|---|
Pages (from-to) | 218-235 |
Number of pages | 18 |
Journal | International Journal of Cancer |
Volume | 19 |
Issue number | 1 |
State | Published - Jan 1 1977 |
ASJC Scopus subject areas
- Oncology
- Cancer Research