Human, nonhuman primate, and rat pancreatic islets express erythropoietin receptors

Elizabeth S. Fenjves, M. Sofia Ochoa, Over Cabrera, Armando J. Mendez, Norma S. Kenyon, Luca Inverardi, Camillo Ricordi

Research output: Contribution to journalArticle

62 Scopus citations

Abstract

Background. Erythropoietin (EPO) promotes survival in a variety of cells by mediating antiapoptotic signals through the EPO receptor (R). The authors examined pancreatic islets for the presence of EPO-R to determine whether these cells are protected by EPO from cytokine-induced apoptosis. Methods. Reverse-transcriptase polymerase chain reaction, immunohistology, and Western blots were used to establish the presence and localization of EPO-R on rat, nonhuman primate, and human islets. Islets were exposed to cytokines in the presence and absence of recombinant EPO and apoptosis was measured using a terminal deoxynucleotide transferase-mediated dUTP nick-end labeling assay followed by fluorescence-activated cell sorter analysis. Glucose stimulation indices were measured to assess the effect of EPO on islet function. Results. The presence of EPO-R was demonstrated on islets regardless of species. Recombinant EPO protected islets in culture from cytokine-induced apoptosis in a dose-dependent manner. Furthermore, the presence of EPO in the media does not adversely affect islet function. Conclusions. This is the first demonstration that pancreatic islets express EPO-R and that EPO may prevent islet-cell apoptosis in culture. In vivo trials to evaluate the potential of long-term expression of EPO to augment islet survival in transplantation are underway.

Original languageEnglish (US)
Pages (from-to)1356-1360
Number of pages5
JournalTransplantation
Volume75
Issue number8
DOIs
StatePublished - Apr 27 2003

ASJC Scopus subject areas

  • Transplantation

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