Human LPLUNC1 is a secreted product of goblet cells and minor glands of the respiratory and upper aerodigestive tracts

Colin D. Bingle, Kirsty Wilson, Hayley Lunn, Frances A. Barnes, Alec S. High, William A. Wallace, Doris Rassl, Michael A Campos, Manuel Ribeiro, Lynne Bingle

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Long PLUNC1 (LPLUNC1, C20orf114) is a member of a family of poorly described proteins (PLUNCS) expressed in the upper respiratory tract and oral cavity, which may function in host defence. Although it is one of the most highly expressed genes in the upper airways and has been identified in sputum and nasal secretions by proteomic studies, localisation of LPLUNC1 protein has not yet been described. We developed affinity purified antibodies and localised the protein in tissues of the human respiratory tract, oro- and nasopharynx. We have complemented these studies with analysis of LPLUNC1 expression in primary human lung cell cultures and used Western blotting to study the protein in cell culture secretions and in BAL. LPLUNC1 is a product of a population of goblet cells in the airway epithelium and nasal passages and is also present in airway submucosal glands and minor glands of the oral and nasal cavities. The protein is not expressed in peripheral lung epithelial cells. LPLUNC1 is present in bronchoalveolar lavage fluid as two glycosylated isoforms and primary airway epithelial cells produce identical proteins as they undergo mucociliary differentiation. Our results suggest that LPLUNC1 is an abundant, secreted product of goblet cells and minor mucosal glands of the respiratory tract and oral cavity and suggest that the protein functions in the complex milieu that protects the mucosal surfaces in these locations.

Original languageEnglish
Pages (from-to)505-515
Number of pages11
JournalHistochemistry and Cell Biology
Volume133
Issue number5
DOIs
StatePublished - May 1 2010

Fingerprint

Goblet Cells
Respiratory System
Proteins
Mouth
Cell Culture Techniques
Epithelial Cells
Dimercaprol
Lung
Oropharynx
Antibody Affinity
Nasopharynx
Nasal Mucosa
Nasal Cavity
Bronchoalveolar Lavage Fluid
Sputum
Nose
Proteomics
Protein Isoforms
Western Blotting
Population

Keywords

  • Airway
  • Glands
  • Goblet cells
  • Immunohistochemistry
  • Innate immunity
  • LPLUNC1

ASJC Scopus subject areas

  • Cell Biology
  • Histology
  • Medical Laboratory Technology
  • Molecular Biology

Cite this

Human LPLUNC1 is a secreted product of goblet cells and minor glands of the respiratory and upper aerodigestive tracts. / Bingle, Colin D.; Wilson, Kirsty; Lunn, Hayley; Barnes, Frances A.; High, Alec S.; Wallace, William A.; Rassl, Doris; Campos, Michael A; Ribeiro, Manuel; Bingle, Lynne.

In: Histochemistry and Cell Biology, Vol. 133, No. 5, 01.05.2010, p. 505-515.

Research output: Contribution to journalArticle

Bingle, CD, Wilson, K, Lunn, H, Barnes, FA, High, AS, Wallace, WA, Rassl, D, Campos, MA, Ribeiro, M & Bingle, L 2010, 'Human LPLUNC1 is a secreted product of goblet cells and minor glands of the respiratory and upper aerodigestive tracts', Histochemistry and Cell Biology, vol. 133, no. 5, pp. 505-515. https://doi.org/10.1007/s00418-010-0683-0
Bingle, Colin D. ; Wilson, Kirsty ; Lunn, Hayley ; Barnes, Frances A. ; High, Alec S. ; Wallace, William A. ; Rassl, Doris ; Campos, Michael A ; Ribeiro, Manuel ; Bingle, Lynne. / Human LPLUNC1 is a secreted product of goblet cells and minor glands of the respiratory and upper aerodigestive tracts. In: Histochemistry and Cell Biology. 2010 ; Vol. 133, No. 5. pp. 505-515.
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