Abstract
The human (hu) λ5 gene is the orthologue of the murine (m) λ5 gene and is transcriptionally active in pro-B and pre-B lymphocytes. We generated huλ5 transgenic mouse lines to address the tissue- and stage-specific molecular transcription regulation and functional capabilities of huλ5. The huλ5 transgenic mice exhibit high levels of huλ5 mRNA expression, restricted to the pro-B and pre-B cell stages, indicating that the 28 kb XhoI transgene contains the necessary transcription elements for tissue- and stage-specific expression. When crossed with mλ5 nullizygous (-/-) mice, it was found that the huλ5 transgene does not rescue the mλ5 (-/-) phenotype of no or very low B lymphocyte numbers. Abelson-derived lines from huλ5 (+/-)/mλ5 (-/-) bone marrow show expression of a chimeric huλ5, mVpreB, m mu (μ) pre-B receptor (pre-BCR) and cell surface expression is indicated. Our results suggest that conformational and/or signaling differences exist between the mpre-BCR and this chimeric pre-BCR and that assembly and surface expression of the pre-BCR is not sufficient for B cell development.
| Original language | English |
|---|---|
| Journal | FASEB Journal |
| Volume | 12 |
| Issue number | 5 |
| State | Published - Mar 20 1998 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Agricultural and Biological Sciences (miscellaneous)
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Cell Biology
Cite this
Human lambda (λ)5 transgene does not rescue the murine λ5 nullizygous phenotype. / Blomberg, Bonnie B; Donohoe, M. E.; Riley, Richard L; Lonberg, N.; Beck-Engeser, G.; Jäck, H. M.
In: FASEB Journal, Vol. 12, No. 5, 20.03.1998.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Human lambda (λ)5 transgene does not rescue the murine λ5 nullizygous phenotype
AU - Blomberg, Bonnie B
AU - Donohoe, M. E.
AU - Riley, Richard L
AU - Lonberg, N.
AU - Beck-Engeser, G.
AU - Jäck, H. M.
PY - 1998/3/20
Y1 - 1998/3/20
N2 - The human (hu) λ5 gene is the orthologue of the murine (m) λ5 gene and is transcriptionally active in pro-B and pre-B lymphocytes. We generated huλ5 transgenic mouse lines to address the tissue- and stage-specific molecular transcription regulation and functional capabilities of huλ5. The huλ5 transgenic mice exhibit high levels of huλ5 mRNA expression, restricted to the pro-B and pre-B cell stages, indicating that the 28 kb XhoI transgene contains the necessary transcription elements for tissue- and stage-specific expression. When crossed with mλ5 nullizygous (-/-) mice, it was found that the huλ5 transgene does not rescue the mλ5 (-/-) phenotype of no or very low B lymphocyte numbers. Abelson-derived lines from huλ5 (+/-)/mλ5 (-/-) bone marrow show expression of a chimeric huλ5, mVpreB, m mu (μ) pre-B receptor (pre-BCR) and cell surface expression is indicated. Our results suggest that conformational and/or signaling differences exist between the mpre-BCR and this chimeric pre-BCR and that assembly and surface expression of the pre-BCR is not sufficient for B cell development.
AB - The human (hu) λ5 gene is the orthologue of the murine (m) λ5 gene and is transcriptionally active in pro-B and pre-B lymphocytes. We generated huλ5 transgenic mouse lines to address the tissue- and stage-specific molecular transcription regulation and functional capabilities of huλ5. The huλ5 transgenic mice exhibit high levels of huλ5 mRNA expression, restricted to the pro-B and pre-B cell stages, indicating that the 28 kb XhoI transgene contains the necessary transcription elements for tissue- and stage-specific expression. When crossed with mλ5 nullizygous (-/-) mice, it was found that the huλ5 transgene does not rescue the mλ5 (-/-) phenotype of no or very low B lymphocyte numbers. Abelson-derived lines from huλ5 (+/-)/mλ5 (-/-) bone marrow show expression of a chimeric huλ5, mVpreB, m mu (μ) pre-B receptor (pre-BCR) and cell surface expression is indicated. Our results suggest that conformational and/or signaling differences exist between the mpre-BCR and this chimeric pre-BCR and that assembly and surface expression of the pre-BCR is not sufficient for B cell development.
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UR - http://www.scopus.com/inward/citedby.url?scp=33749323557&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:33749323557
VL - 12
JO - FASEB Journal
JF - FASEB Journal
SN - 0892-6638
IS - 5
ER -