Human lambda (λ)5 transgene does not rescue the murine λ5 nullizygous phenotype

B. B. Blomberg, M. E. Donohoe, R. Riley, N. Lonberg, G. Beck-Engeser, H. M. Jäck

Research output: Contribution to journalArticlepeer-review

Abstract

The human (hu) λ5 gene is the orthologue of the murine (m) λ5 gene and is transcriptionally active in pro-B and pre-B lymphocytes. We generated huλ5 transgenic mouse lines to address the tissue- and stage-specific molecular transcription regulation and functional capabilities of huλ5. The huλ5 transgenic mice exhibit high levels of huλ5 mRNA expression, restricted to the pro-B and pre-B cell stages, indicating that the 28 kb XhoI transgene contains the necessary transcription elements for tissue- and stage-specific expression. When crossed with mλ5 nullizygous (-/-) mice, it was found that the huλ5 transgene does not rescue the mλ5 (-/-) phenotype of no or very low B lymphocyte numbers. Abelson-derived lines from huλ5 (+/-)/mλ5 (-/-) bone marrow show expression of a chimeric huλ5, mVpreB, m mu (μ) pre-B receptor (pre-BCR) and cell surface expression is indicated. Our results suggest that conformational and/or signaling differences exist between the mpre-BCR and this chimeric pre-BCR and that assembly and surface expression of the pre-BCR is not sufficient for B cell development.

Original languageEnglish (US)
Pages (from-to)A1073
JournalFASEB Journal
Volume12
Issue number5
StatePublished - Mar 20 1998
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

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