Human lambda (λ)5 transgene does not rescue the murine λ5 nullizygous phenotype

Bonnie B Blomberg, M. E. Donohoe, Richard L Riley, N. Lonberg, G. Beck-Engeser, H. M. Jäck

Research output: Contribution to journalArticle

Abstract

The human (hu) λ5 gene is the orthologue of the murine (m) λ5 gene and is transcriptionally active in pro-B and pre-B lymphocytes. We generated huλ5 transgenic mouse lines to address the tissue- and stage-specific molecular transcription regulation and functional capabilities of huλ5. The huλ5 transgenic mice exhibit high levels of huλ5 mRNA expression, restricted to the pro-B and pre-B cell stages, indicating that the 28 kb XhoI transgene contains the necessary transcription elements for tissue- and stage-specific expression. When crossed with mλ5 nullizygous (-/-) mice, it was found that the huλ5 transgene does not rescue the mλ5 (-/-) phenotype of no or very low B lymphocyte numbers. Abelson-derived lines from huλ5 (+/-)/mλ5 (-/-) bone marrow show expression of a chimeric huλ5, mVpreB, m mu (μ) pre-B receptor (pre-BCR) and cell surface expression is indicated. Our results suggest that conformational and/or signaling differences exist between the mpre-BCR and this chimeric pre-BCR and that assembly and surface expression of the pre-BCR is not sufficient for B cell development.

Original languageEnglish
JournalFASEB Journal
Volume12
Issue number5
StatePublished - Mar 20 1998
Externally publishedYes

Fingerprint

B-Lymphoid Precursor Cells
Lymphocytes
Transcription
Transgenes
Transgenic Mice
transgenes
B-lymphocytes
Pre-B Cell Receptors
B-Lymphocytes
Genes
Cells
Tissue
Phenotype
phenotype
mice
Lymphocyte Count
receptors
Bone
transcription (genetics)
Bone Marrow

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Blomberg, B. B., Donohoe, M. E., Riley, R. L., Lonberg, N., Beck-Engeser, G., & Jäck, H. M. (1998). Human lambda (λ)5 transgene does not rescue the murine λ5 nullizygous phenotype. FASEB Journal, 12(5).

Human lambda (λ)5 transgene does not rescue the murine λ5 nullizygous phenotype. / Blomberg, Bonnie B; Donohoe, M. E.; Riley, Richard L; Lonberg, N.; Beck-Engeser, G.; Jäck, H. M.

In: FASEB Journal, Vol. 12, No. 5, 20.03.1998.

Research output: Contribution to journalArticle

Blomberg, BB, Donohoe, ME, Riley, RL, Lonberg, N, Beck-Engeser, G & Jäck, HM 1998, 'Human lambda (λ)5 transgene does not rescue the murine λ5 nullizygous phenotype', FASEB Journal, vol. 12, no. 5.
Blomberg, Bonnie B ; Donohoe, M. E. ; Riley, Richard L ; Lonberg, N. ; Beck-Engeser, G. ; Jäck, H. M. / Human lambda (λ)5 transgene does not rescue the murine λ5 nullizygous phenotype. In: FASEB Journal. 1998 ; Vol. 12, No. 5.
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