Human islet amyloid polypeptide gene: Complete nucleotide sequence, chromosomal localization, and evolutionary history

Masahiro Nishi, Tokio Sanke, Susumu Seino, Roger L. Eddy, Yao Shan Fan, Mary G. Byers, Thomas B. Shows, Graeme I. Bell, Donald F. Steiner

Research output: Contribution to journalArticle

86 Scopus citations

Abstract

The gene-encoding human islet amyloid polypeptide (hIAPP), a recently discovered 37 amino acid hormone-like polypeptide which is expressed in the insulin-producing β-cells of the endocrine pancreas, has been isolated and characterized. The coding region of the gene is interrupted in the 5'-untranslated region and NH2-terminal propeptide by introns of 330 and 4808 base pairs (bp), respectively. Exon 1 (104 bp) encodes most of the 5'-untranslated region of the mRNA; exon 2 (95 bp) encodes 15 nucleotides of 5'-untranslated region, the putative 22 amino acid signal peptide and five residues of the NH2-terminal propeptide; exon 3 (1246 bp) encodes the remainder of the NH2-terminal propeptide (residues 6-9), the IAPP moiety and its processing signals and the 16 amino acid COOH-terminal propeptide, as well as the 3'-untranslated region of the mRNA (1059 bp). Analysis of the nucleotide and predicted amino acid sequence of intron 2 of the hIAPP gene did not reveal any homology with the structurally related calcitonin/calcitonin-gene-related peptide genes and indicates that, in contrast to these latter genes, the hIAPP gene apparently gives rise to only a single hormonal product. The transcriptional initiation site was identified about 28 bp downstream from a TATAA sequence. The hIAPP gene was localized to the p12.3 region of chromosome 12.

Original languageEnglish (US)
Pages (from-to)1775-1781
Number of pages7
JournalMolecular Endocrinology
Volume3
Issue number11
DOIs
StatePublished - Nov 1989
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology

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    Nishi, M., Sanke, T., Seino, S., Eddy, R. L., Fan, Y. S., Byers, M. G., Shows, T. B., Bell, G. I., & Steiner, D. F. (1989). Human islet amyloid polypeptide gene: Complete nucleotide sequence, chromosomal localization, and evolutionary history. Molecular Endocrinology, 3(11), 1775-1781. https://doi.org/10.1210/mend-3-11-1775