Human islet amyloid polypeptide gene: Complete nucleotide sequence, chromosomal localization, and evolutionary history

M. Nishi, T. Sanke, S. Seino, R. L. Eddy, Yao-Shan Fan, M. G. Byers, T. B. Shows, G. I. Bell, D. F. Steiner

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

The gene-encoding human islet amyloid polypeptide (hIAPP), a recently discovered 37 amino acid hormone-like polypeptide which is expressed in the insulin-producing β-cells of the endocrine pancreas, has been isolated and characterized. The coding region of the gene is interrupted in the 5'-untranslated region and NH2-terminal propeptide by introns of 330 and 4808 base pairs (bp), respectively. Exon 1 (104 bp) encodes most of the 5'-untranslated region of the mRNA; exon 2 (95 bp) encodes 15 nucleotides of 5'-untranslated region, the putative 22 amino acid signal peptide and five residues of the NH2-terminal propeptide; exon 3 (1246 bp) encodes the remainder of the NH2-terminal propeptide (residues 6-9), the IAPP moiety and its processing signals and the 16 amino acid COOH-terminal propeptide, as well as the 3'-untranslated region of the mRNA (1059 bp). Analysis of the nucleotide and predicted amino acid sequence of intron 2 of the hIAPP gene did not reveal any homology with the structurally related calcitonin/calcitonin-gene-related peptide genes and indicates that, in contrast to these latter genes, the hIAPP gene apparently gives rise to only a single hormonal product. The transcriptional initiation site was identified about 28 bp downstream from a TATAA sequence. The hIAPP gene was localized to the p12.3 region of chromosome 12.

Original languageEnglish
Pages (from-to)1775-1781
Number of pages7
JournalMolecular Endocrinology
Volume3
Issue number11
StatePublished - Dec 15 1989
Externally publishedYes

Fingerprint

Islet Amyloid Polypeptide
Base Pairing
History
5' Untranslated Regions
Genes
Exons
Amino Acids
Introns
Nucleotides
Chromosomes, Human, Pair 12
Messenger RNA
Peptide Hormones
Calcitonin Gene-Related Peptide
Calcitonin
3' Untranslated Regions
Protein Sorting Signals
Islets of Langerhans
Amino Acid Sequence
Insulin

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology, Diabetes and Metabolism

Cite this

Nishi, M., Sanke, T., Seino, S., Eddy, R. L., Fan, Y-S., Byers, M. G., ... Steiner, D. F. (1989). Human islet amyloid polypeptide gene: Complete nucleotide sequence, chromosomal localization, and evolutionary history. Molecular Endocrinology, 3(11), 1775-1781.

Human islet amyloid polypeptide gene : Complete nucleotide sequence, chromosomal localization, and evolutionary history. / Nishi, M.; Sanke, T.; Seino, S.; Eddy, R. L.; Fan, Yao-Shan; Byers, M. G.; Shows, T. B.; Bell, G. I.; Steiner, D. F.

In: Molecular Endocrinology, Vol. 3, No. 11, 15.12.1989, p. 1775-1781.

Research output: Contribution to journalArticle

Nishi, M, Sanke, T, Seino, S, Eddy, RL, Fan, Y-S, Byers, MG, Shows, TB, Bell, GI & Steiner, DF 1989, 'Human islet amyloid polypeptide gene: Complete nucleotide sequence, chromosomal localization, and evolutionary history', Molecular Endocrinology, vol. 3, no. 11, pp. 1775-1781.
Nishi, M. ; Sanke, T. ; Seino, S. ; Eddy, R. L. ; Fan, Yao-Shan ; Byers, M. G. ; Shows, T. B. ; Bell, G. I. ; Steiner, D. F. / Human islet amyloid polypeptide gene : Complete nucleotide sequence, chromosomal localization, and evolutionary history. In: Molecular Endocrinology. 1989 ; Vol. 3, No. 11. pp. 1775-1781.
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