Human immunodeficiency virus type 1 envelope glycoproteins gp120 and gp160 induce interleukin-6 production in CD4+ T-cell clones

Naoki Oyaizu, Narendra Chirmule, Yoko Ohnishi, Vaniambadi S. Kalyanaraman, Savita G Pahwa

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Polyclonal B-cell activation is a characteristic feature of AIDS and of the AIDS-related complex. Since the immunoregulatory cytokine interleukin-6 (IL-6) plays a major role in inducing B-cell differentiation, we examined the effects of native human immunodeficiency virus type 1 envelope glycoproteins gp120 and gp160 on IL-6 induction. In this study, we have demonstrated that both gp120 and gp160 have the ability to induce IL-6 mRNA and biologically active IL-6 protein secretion in peripheral blood mononuclear cells in vitro. The envelope protein preparations had no detectable endotoxin as tested by the Limulus amebocyte lysate assay, and hence we can rule out the effect of contaminating endotoxin, which is a potent inducer of IL-6 in monocyte/macrophage cell cultures. In addition, we have shown that the envelope glycoproteins act directly on CD4+-cloned T cells to induce IL-6 production in the absence of monocytes. These findings indicate that monocytes and T cells both contribute to the secretion of IL-6, which plays an important role in the pathogenesis of B-cell activation in human immunodeficiency virus infection.

Original languageEnglish
Pages (from-to)6277-6282
Number of pages6
JournalJournal of Virology
Volume65
Issue number11
StatePublished - Nov 1 1991
Externally publishedYes

Fingerprint

Human immunodeficiency virus 1
interleukin-6
HIV-1
glycoproteins
Interleukin-6
Glycoproteins
Clone Cells
T-lymphocytes
clones
T-Lymphocytes
monocytes
B-lymphocytes
Monocytes
B-Lymphocytes
endotoxins
Endotoxins
Limulus
AIDS-Related Complex
Horseshoe Crabs
protein secretion

ASJC Scopus subject areas

  • Immunology

Cite this

Human immunodeficiency virus type 1 envelope glycoproteins gp120 and gp160 induce interleukin-6 production in CD4+ T-cell clones. / Oyaizu, Naoki; Chirmule, Narendra; Ohnishi, Yoko; Kalyanaraman, Vaniambadi S.; Pahwa, Savita G.

In: Journal of Virology, Vol. 65, No. 11, 01.11.1991, p. 6277-6282.

Research output: Contribution to journalArticle

Oyaizu, Naoki ; Chirmule, Narendra ; Ohnishi, Yoko ; Kalyanaraman, Vaniambadi S. ; Pahwa, Savita G. / Human immunodeficiency virus type 1 envelope glycoproteins gp120 and gp160 induce interleukin-6 production in CD4+ T-cell clones. In: Journal of Virology. 1991 ; Vol. 65, No. 11. pp. 6277-6282.
@article{d77f09cde1c3450d844a3dfcb2c6f83b,
title = "Human immunodeficiency virus type 1 envelope glycoproteins gp120 and gp160 induce interleukin-6 production in CD4+ T-cell clones",
abstract = "Polyclonal B-cell activation is a characteristic feature of AIDS and of the AIDS-related complex. Since the immunoregulatory cytokine interleukin-6 (IL-6) plays a major role in inducing B-cell differentiation, we examined the effects of native human immunodeficiency virus type 1 envelope glycoproteins gp120 and gp160 on IL-6 induction. In this study, we have demonstrated that both gp120 and gp160 have the ability to induce IL-6 mRNA and biologically active IL-6 protein secretion in peripheral blood mononuclear cells in vitro. The envelope protein preparations had no detectable endotoxin as tested by the Limulus amebocyte lysate assay, and hence we can rule out the effect of contaminating endotoxin, which is a potent inducer of IL-6 in monocyte/macrophage cell cultures. In addition, we have shown that the envelope glycoproteins act directly on CD4+-cloned T cells to induce IL-6 production in the absence of monocytes. These findings indicate that monocytes and T cells both contribute to the secretion of IL-6, which plays an important role in the pathogenesis of B-cell activation in human immunodeficiency virus infection.",
author = "Naoki Oyaizu and Narendra Chirmule and Yoko Ohnishi and Kalyanaraman, {Vaniambadi S.} and Pahwa, {Savita G}",
year = "1991",
month = "11",
day = "1",
language = "English",
volume = "65",
pages = "6277--6282",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "11",

}

TY - JOUR

T1 - Human immunodeficiency virus type 1 envelope glycoproteins gp120 and gp160 induce interleukin-6 production in CD4+ T-cell clones

AU - Oyaizu, Naoki

AU - Chirmule, Narendra

AU - Ohnishi, Yoko

AU - Kalyanaraman, Vaniambadi S.

AU - Pahwa, Savita G

PY - 1991/11/1

Y1 - 1991/11/1

N2 - Polyclonal B-cell activation is a characteristic feature of AIDS and of the AIDS-related complex. Since the immunoregulatory cytokine interleukin-6 (IL-6) plays a major role in inducing B-cell differentiation, we examined the effects of native human immunodeficiency virus type 1 envelope glycoproteins gp120 and gp160 on IL-6 induction. In this study, we have demonstrated that both gp120 and gp160 have the ability to induce IL-6 mRNA and biologically active IL-6 protein secretion in peripheral blood mononuclear cells in vitro. The envelope protein preparations had no detectable endotoxin as tested by the Limulus amebocyte lysate assay, and hence we can rule out the effect of contaminating endotoxin, which is a potent inducer of IL-6 in monocyte/macrophage cell cultures. In addition, we have shown that the envelope glycoproteins act directly on CD4+-cloned T cells to induce IL-6 production in the absence of monocytes. These findings indicate that monocytes and T cells both contribute to the secretion of IL-6, which plays an important role in the pathogenesis of B-cell activation in human immunodeficiency virus infection.

AB - Polyclonal B-cell activation is a characteristic feature of AIDS and of the AIDS-related complex. Since the immunoregulatory cytokine interleukin-6 (IL-6) plays a major role in inducing B-cell differentiation, we examined the effects of native human immunodeficiency virus type 1 envelope glycoproteins gp120 and gp160 on IL-6 induction. In this study, we have demonstrated that both gp120 and gp160 have the ability to induce IL-6 mRNA and biologically active IL-6 protein secretion in peripheral blood mononuclear cells in vitro. The envelope protein preparations had no detectable endotoxin as tested by the Limulus amebocyte lysate assay, and hence we can rule out the effect of contaminating endotoxin, which is a potent inducer of IL-6 in monocyte/macrophage cell cultures. In addition, we have shown that the envelope glycoproteins act directly on CD4+-cloned T cells to induce IL-6 production in the absence of monocytes. These findings indicate that monocytes and T cells both contribute to the secretion of IL-6, which plays an important role in the pathogenesis of B-cell activation in human immunodeficiency virus infection.

UR - http://www.scopus.com/inward/record.url?scp=0025985917&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025985917&partnerID=8YFLogxK

M3 - Article

VL - 65

SP - 6277

EP - 6282

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 11

ER -