Human endothelin ETA receptor antisense oligodeoxynucleotides inhibit endothelin-1 evoked vasoconstriction

Mikael Adner, David Erlinge, Leif G. Salford, Frances Yee, Claes R Wahlestedt, Lars Edvinsson

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Antisense oligodeoxynucleotides to endothelin ETA receptor mRNA were used to characterize vascular smooth muscle receptors. The concentration-response curve showed a significant attenuation of endothelin-1-induced contraction in circular segments of the human superficial temporal artery. Endothelin ETB receptor antisense or mismatch oligodeoxynucleotides showed no alteration of the endothelin-1-induced contraction. Complementary experiments with the selective endothelin ETA receptor antagonist FR139317 demonstrated a shift of the concentration-response curve to the right in a competitive manner (pA2 = 6.93). The specific method of using the receptor antisense oligodeoxynucleotides approach revealed the presence of endothelin ETA receptors mediating contraction in the human superficial temporal artery.

Original languageEnglish
Pages (from-to)281-284
Number of pages4
JournalEuropean Journal of Pharmacology
Volume261
Issue number3
DOIs
StatePublished - Aug 22 1994
Externally publishedYes

Fingerprint

Endothelin Receptors
Oligodeoxyribonucleotides
Endothelin-1
Vasoconstriction
Temporal Arteries
Vascular Smooth Muscle
Messenger RNA

Keywords

  • Antisense oligodeoxynucleotide
  • Endothelin ET receptor
  • Temporal artery, human

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

Cite this

Human endothelin ETA receptor antisense oligodeoxynucleotides inhibit endothelin-1 evoked vasoconstriction. / Adner, Mikael; Erlinge, David; Salford, Leif G.; Yee, Frances; Wahlestedt, Claes R; Edvinsson, Lars.

In: European Journal of Pharmacology, Vol. 261, No. 3, 22.08.1994, p. 281-284.

Research output: Contribution to journalArticle

Adner, Mikael ; Erlinge, David ; Salford, Leif G. ; Yee, Frances ; Wahlestedt, Claes R ; Edvinsson, Lars. / Human endothelin ETA receptor antisense oligodeoxynucleotides inhibit endothelin-1 evoked vasoconstriction. In: European Journal of Pharmacology. 1994 ; Vol. 261, No. 3. pp. 281-284.
@article{7d523774de9e43dda49bdcb02b53bbb5,
title = "Human endothelin ETA receptor antisense oligodeoxynucleotides inhibit endothelin-1 evoked vasoconstriction",
abstract = "Antisense oligodeoxynucleotides to endothelin ETA receptor mRNA were used to characterize vascular smooth muscle receptors. The concentration-response curve showed a significant attenuation of endothelin-1-induced contraction in circular segments of the human superficial temporal artery. Endothelin ETB receptor antisense or mismatch oligodeoxynucleotides showed no alteration of the endothelin-1-induced contraction. Complementary experiments with the selective endothelin ETA receptor antagonist FR139317 demonstrated a shift of the concentration-response curve to the right in a competitive manner (pA2 = 6.93). The specific method of using the receptor antisense oligodeoxynucleotides approach revealed the presence of endothelin ETA receptors mediating contraction in the human superficial temporal artery.",
keywords = "Antisense oligodeoxynucleotide, Endothelin ET receptor, Temporal artery, human",
author = "Mikael Adner and David Erlinge and Salford, {Leif G.} and Frances Yee and Wahlestedt, {Claes R} and Lars Edvinsson",
year = "1994",
month = "8",
day = "22",
doi = "10.1016/0014-2999(94)90118-X",
language = "English",
volume = "261",
pages = "281--284",
journal = "European Journal of Pharmacology",
issn = "0014-2999",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - Human endothelin ETA receptor antisense oligodeoxynucleotides inhibit endothelin-1 evoked vasoconstriction

AU - Adner, Mikael

AU - Erlinge, David

AU - Salford, Leif G.

AU - Yee, Frances

AU - Wahlestedt, Claes R

AU - Edvinsson, Lars

PY - 1994/8/22

Y1 - 1994/8/22

N2 - Antisense oligodeoxynucleotides to endothelin ETA receptor mRNA were used to characterize vascular smooth muscle receptors. The concentration-response curve showed a significant attenuation of endothelin-1-induced contraction in circular segments of the human superficial temporal artery. Endothelin ETB receptor antisense or mismatch oligodeoxynucleotides showed no alteration of the endothelin-1-induced contraction. Complementary experiments with the selective endothelin ETA receptor antagonist FR139317 demonstrated a shift of the concentration-response curve to the right in a competitive manner (pA2 = 6.93). The specific method of using the receptor antisense oligodeoxynucleotides approach revealed the presence of endothelin ETA receptors mediating contraction in the human superficial temporal artery.

AB - Antisense oligodeoxynucleotides to endothelin ETA receptor mRNA were used to characterize vascular smooth muscle receptors. The concentration-response curve showed a significant attenuation of endothelin-1-induced contraction in circular segments of the human superficial temporal artery. Endothelin ETB receptor antisense or mismatch oligodeoxynucleotides showed no alteration of the endothelin-1-induced contraction. Complementary experiments with the selective endothelin ETA receptor antagonist FR139317 demonstrated a shift of the concentration-response curve to the right in a competitive manner (pA2 = 6.93). The specific method of using the receptor antisense oligodeoxynucleotides approach revealed the presence of endothelin ETA receptors mediating contraction in the human superficial temporal artery.

KW - Antisense oligodeoxynucleotide

KW - Endothelin ET receptor

KW - Temporal artery, human

UR - http://www.scopus.com/inward/record.url?scp=0028169863&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028169863&partnerID=8YFLogxK

U2 - 10.1016/0014-2999(94)90118-X

DO - 10.1016/0014-2999(94)90118-X

M3 - Article

VL - 261

SP - 281

EP - 284

JO - European Journal of Pharmacology

JF - European Journal of Pharmacology

SN - 0014-2999

IS - 3

ER -