Human endothelial cell interactions with surface‐coupled adhesion peptides on a nonadhesive glass substrate and two polymeric biomaterials

S. P. Massia, J. A. Hubbell

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247 Scopus citations

Abstract

The attachment, spreading, spreading rate, focal contact formation, and cytoskeletal organization of human umbilical vein endothelial cells (HUVECs) were investigated on substrates that had been covalently grafted with the cell adhesion peptides Arg-Gly-Asp (RGD) and Tyr-Ile-Gly-Ser-Arg (YIGSR). This approach was used to provide substrates that were adhesive to cells even in the absence of serum proteins and with no prior pretreatment of the surface with proteins of the cell adhesion molecule (CAM) family. It is shown that although PET and PTFE-OH supported adhesion when incubated in serum-supplemented medium, neither of these materials supported adhesion with only albumin present, indicating that cell adhesion is mediated by adsorbed CAM proteins.

Original languageEnglish (US)
Pages (from-to)223-242
Number of pages20
JournalJournal of Biomedical Materials Research
Volume25
Issue number2
DOIs
StatePublished - Feb 1991

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ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering

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