Human endothelial cell interactions with surface‐coupled adhesion peptides on a nonadhesive glass substrate and two polymeric biomaterials

S. P. Massia, J. A. Hubbell

Research output: Contribution to journalArticle

247 Scopus citations


The attachment, spreading, spreading rate, focal contact formation, and cytoskeletal organization of human umbilical vein endothelial cells (HUVECs) were investigated on substrates that had been covalently grafted with the cell adhesion peptides Arg-Gly-Asp (RGD) and Tyr-Ile-Gly-Ser-Arg (YIGSR). This approach was used to provide substrates that were adhesive to cells even in the absence of serum proteins and with no prior pretreatment of the surface with proteins of the cell adhesion molecule (CAM) family. It is shown that although PET and PTFE-OH supported adhesion when incubated in serum-supplemented medium, neither of these materials supported adhesion with only albumin present, indicating that cell adhesion is mediated by adsorbed CAM proteins.

Original languageEnglish (US)
Pages (from-to)223-242
Number of pages20
JournalJournal of Biomedical Materials Research
Issue number2
StatePublished - Feb 1991


ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering

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