Human embryonic stem cell derivation from poor-quality embryos

Paul H. Lerou; Akiko Yabuuchi; Hongguang Huo; Ayumu Takeuchi; Jessica Shea; Tina Cimini; Tan A. Ince; Elizabeth Ginsburg; Catherine Racowsky; George Q. Daley

doi:10.1038/nbt1378

Human embryonic stem cell derivation from poor-quality embryos

Paul H. Lerou, Akiko Yabuuchi, Hongguang Huo, Ayumu Takeuchi, Jessica Shea, Tina Cimini, Tan A. Ince, Elizabeth Ginsburg, Catherine Racowsky, George Q. Daley

Research output: Contribution to journal › Article › peer-review

80 Scopus citations

Abstract

During in vitro fertilization, embryos deemed clinically useless based on poor morphology are typically discarded. Here we demonstrate a statistical correlation between the developmental stage of such poor-quality embryos and the yield of human embryonic stem (hES) cell lines. Early-arrested or highly fragmented embryos only rarely yield cell lines, whereas those that have achieved blastocyst stage are a robust source of normal hES cells.

Original language	English (US)
Pages (from-to)	212-214
Number of pages	3
Journal	Nature Biotechnology
Volume	26
Issue number	2
DOIs	https://doi.org/10.1038/nbt1378
State	Published - Feb 2008
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology
Molecular Medicine
Biomedical Engineering

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10.1038/nbt1378

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title = "Human embryonic stem cell derivation from poor-quality embryos",

abstract = "During in vitro fertilization, embryos deemed clinically useless based on poor morphology are typically discarded. Here we demonstrate a statistical correlation between the developmental stage of such poor-quality embryos and the yield of human embryonic stem (hES) cell lines. Early-arrested or highly fragmented embryos only rarely yield cell lines, whereas those that have achieved blastocyst stage are a robust source of normal hES cells.",

author = "Lerou, {Paul H.} and Akiko Yabuuchi and Hongguang Huo and Ayumu Takeuchi and Jessica Shea and Tina Cimini and Ince, {Tan A.} and Elizabeth Ginsburg and Catherine Racowsky and Daley, {George Q.}",

note = "Funding Information: This work was made possible through the generosity and vision of Joshua and Anita Bekenstein, the Harvard Stem Cell Institute and Children{\textquoteright}s Hospital Boston. G.Q.D. is a recipient of the Burroughs Wellcome Fund Clinical Scientist Award in Translational Research. T.A.I. was supported by grants from the Breast Cancer Research Foundation and Mesothelioma Applied Research Foundation. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.",

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language = "English (US)",

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AU - Lerou, Paul H.

AU - Yabuuchi, Akiko

AU - Huo, Hongguang

AU - Takeuchi, Ayumu

AU - Shea, Jessica

AU - Cimini, Tina

AU - Ince, Tan A.

AU - Ginsburg, Elizabeth

AU - Racowsky, Catherine

AU - Daley, George Q.

N1 - Funding Information: This work was made possible through the generosity and vision of Joshua and Anita Bekenstein, the Harvard Stem Cell Institute and Children’s Hospital Boston. G.Q.D. is a recipient of the Burroughs Wellcome Fund Clinical Scientist Award in Translational Research. T.A.I. was supported by grants from the Breast Cancer Research Foundation and Mesothelioma Applied Research Foundation. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.

PY - 2008/2

Y1 - 2008/2

N2 - During in vitro fertilization, embryos deemed clinically useless based on poor morphology are typically discarded. Here we demonstrate a statistical correlation between the developmental stage of such poor-quality embryos and the yield of human embryonic stem (hES) cell lines. Early-arrested or highly fragmented embryos only rarely yield cell lines, whereas those that have achieved blastocyst stage are a robust source of normal hES cells.

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