Abstract
Objectives: To study the effect of cell differentiation on the vulnerability of human neural cell types to human cytomegalovirus (HCMV) infection. Study Design/Methods: Primary cultures of human fetal neuroepithelial stem cells and differentiating neuroepithelial precursor cells were infected with HCMV strain AD169. Infectious virus production, apoptosis, and viral-associated cytopathic effects then were examined over a 5-day period. Results: HCMV established productive infection in these cells, generating 10-fold amplification of infectious virus. There was no significant difference in the percentage of apoptotic cells in HCMV-infected versus mock-infected cultures. HCMV antigen and specific cytopathic effects were observed in differentiating astrocytes and neurons, although HCMV antigen was 2-fold more frequent among postmitotic neurons. Conclusions: Neuroepithelial precursor cells and differentiating astrocytes and neurons are permissive to cytopathic HCMV infection, suggesting that the fetal human central nervous system is vulnerable to HCMV-induced neuronal injury at its earliest stages of development. (C) Lippincott Williams and Wilkins, Inc.
| Original language | English |
|---|---|
| Pages (from-to) | 215-228 |
| Number of pages | 14 |
| Journal | Journal of Human Virology |
| Volume | 3 |
| Issue number | 4 |
| State | Published - Jan 1 2000 |
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Keywords
- Astrocytes
- Cytomegalovirus
- Herpesvirus infections
- Neurons
- Stem cells
ASJC Scopus subject areas
- Virology
Cite this
Human cytomegalovirus causes productive infection and neuronal injury in differentiating fetal human central nervous system neuroepithelial precursor cells. / McCarthy, Micheline; Auger, D.; Whittemore, S. R.
In: Journal of Human Virology, Vol. 3, No. 4, 01.01.2000, p. 215-228.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Human cytomegalovirus causes productive infection and neuronal injury in differentiating fetal human central nervous system neuroepithelial precursor cells
AU - McCarthy, Micheline
AU - Auger, D.
AU - Whittemore, S. R.
PY - 2000/1/1
Y1 - 2000/1/1
N2 - Objectives: To study the effect of cell differentiation on the vulnerability of human neural cell types to human cytomegalovirus (HCMV) infection. Study Design/Methods: Primary cultures of human fetal neuroepithelial stem cells and differentiating neuroepithelial precursor cells were infected with HCMV strain AD169. Infectious virus production, apoptosis, and viral-associated cytopathic effects then were examined over a 5-day period. Results: HCMV established productive infection in these cells, generating 10-fold amplification of infectious virus. There was no significant difference in the percentage of apoptotic cells in HCMV-infected versus mock-infected cultures. HCMV antigen and specific cytopathic effects were observed in differentiating astrocytes and neurons, although HCMV antigen was 2-fold more frequent among postmitotic neurons. Conclusions: Neuroepithelial precursor cells and differentiating astrocytes and neurons are permissive to cytopathic HCMV infection, suggesting that the fetal human central nervous system is vulnerable to HCMV-induced neuronal injury at its earliest stages of development. (C) Lippincott Williams and Wilkins, Inc.
AB - Objectives: To study the effect of cell differentiation on the vulnerability of human neural cell types to human cytomegalovirus (HCMV) infection. Study Design/Methods: Primary cultures of human fetal neuroepithelial stem cells and differentiating neuroepithelial precursor cells were infected with HCMV strain AD169. Infectious virus production, apoptosis, and viral-associated cytopathic effects then were examined over a 5-day period. Results: HCMV established productive infection in these cells, generating 10-fold amplification of infectious virus. There was no significant difference in the percentage of apoptotic cells in HCMV-infected versus mock-infected cultures. HCMV antigen and specific cytopathic effects were observed in differentiating astrocytes and neurons, although HCMV antigen was 2-fold more frequent among postmitotic neurons. Conclusions: Neuroepithelial precursor cells and differentiating astrocytes and neurons are permissive to cytopathic HCMV infection, suggesting that the fetal human central nervous system is vulnerable to HCMV-induced neuronal injury at its earliest stages of development. (C) Lippincott Williams and Wilkins, Inc.
KW - Astrocytes
KW - Cytomegalovirus
KW - Herpesvirus infections
KW - Neurons
KW - Stem cells
UR - http://www.scopus.com/inward/record.url?scp=0033807122&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033807122&partnerID=8YFLogxK
M3 - Article
C2 - 10990168
AN - SCOPUS:0033807122
VL - 3
SP - 215
EP - 228
JO - Journal of Human Virology
JF - Journal of Human Virology
SN - 1090-9508
IS - 4
ER -