Human circulating peripheral blood mononuclear cells for calvarial bone tissue engineering

Harvey Chim, Jan Thorsten Schantz

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

BACKGROUND: Large-scale production of engineered tissues requires an adequate source of expandable cells. Current strategies that involve harvesting of cells from donor tissue or bone marrow for tissue engineering are invasive and unfeasible for obtaining large quantities of cells in a clinical setting. Peripheral blood has been reported to contain circulating hematopoietic cells as well as, in significantly smaller quantities, mesenchymal cells METHODS: An adherent subset of CD14 mononuclear cells was isolated from human peripheral venous blood and characterized in vitro by light microscopy, immunohistochemistry, flow cytometry, and quantitative differentiation assays. These cells were then evaluated for the purposes of tissue engineering in a rat calvarial defect model, in combination with biodegradable polymer matrices made from poly-e-caprolactone. Specimens were analyzed 6 weeks after implantation with histologic analysis, microcomputed tomography, and HLA immunostaining. RESULTS: CD14 mononuclear cells were induced to differentiate into osteoblast-like cells in vitro, with areas of mineralization. In a rat calvarial defect model, tissue-engineered bone with evidence of mineralization was formed within 6 weeks. HLA immunohistochemistry demonstrated that de novo bone formation originated from the transplanted human cells. CONCLUSIONS: These findings show, for the first time, to our knowledge, the derivation of bone from human blood. They also demonstrate the utility of circulating mononuclear cells as a minimally invasive, potentially unlimited pool of cells for tissue engineering and organ regeneration.

Original languageEnglish (US)
Pages (from-to)468-478
Number of pages11
JournalPlastic and Reconstructive Surgery
Volume117
Issue number2
DOIs
StatePublished - Feb 2006
Externally publishedYes

Fingerprint

Tissue Engineering
Blood Cells
Bone and Bones
Immunohistochemistry
Cell Engineering
X-Ray Microtomography
Osteoblasts
Osteogenesis
Regeneration
Microscopy
Flow Cytometry
Polymers
Bone Marrow
Tissue Donors
Light

ASJC Scopus subject areas

  • Surgery

Cite this

Human circulating peripheral blood mononuclear cells for calvarial bone tissue engineering. / Chim, Harvey; Schantz, Jan Thorsten.

In: Plastic and Reconstructive Surgery, Vol. 117, No. 2, 02.2006, p. 468-478.

Research output: Contribution to journalArticle

Chim, Harvey ; Schantz, Jan Thorsten. / Human circulating peripheral blood mononuclear cells for calvarial bone tissue engineering. In: Plastic and Reconstructive Surgery. 2006 ; Vol. 117, No. 2. pp. 468-478.
@article{b8e27fb1228b41d9bd216fdc28c090c2,
title = "Human circulating peripheral blood mononuclear cells for calvarial bone tissue engineering",
abstract = "BACKGROUND: Large-scale production of engineered tissues requires an adequate source of expandable cells. Current strategies that involve harvesting of cells from donor tissue or bone marrow for tissue engineering are invasive and unfeasible for obtaining large quantities of cells in a clinical setting. Peripheral blood has been reported to contain circulating hematopoietic cells as well as, in significantly smaller quantities, mesenchymal cells METHODS: An adherent subset of CD14 mononuclear cells was isolated from human peripheral venous blood and characterized in vitro by light microscopy, immunohistochemistry, flow cytometry, and quantitative differentiation assays. These cells were then evaluated for the purposes of tissue engineering in a rat calvarial defect model, in combination with biodegradable polymer matrices made from poly-e-caprolactone. Specimens were analyzed 6 weeks after implantation with histologic analysis, microcomputed tomography, and HLA immunostaining. RESULTS: CD14 mononuclear cells were induced to differentiate into osteoblast-like cells in vitro, with areas of mineralization. In a rat calvarial defect model, tissue-engineered bone with evidence of mineralization was formed within 6 weeks. HLA immunohistochemistry demonstrated that de novo bone formation originated from the transplanted human cells. CONCLUSIONS: These findings show, for the first time, to our knowledge, the derivation of bone from human blood. They also demonstrate the utility of circulating mononuclear cells as a minimally invasive, potentially unlimited pool of cells for tissue engineering and organ regeneration.",
author = "Harvey Chim and Schantz, {Jan Thorsten}",
year = "2006",
month = "2",
doi = "10.1097/01.prs.0000201489.65811.e7",
language = "English (US)",
volume = "117",
pages = "468--478",
journal = "Plastic and Reconstructive Surgery",
issn = "0032-1052",
publisher = "Lippincott Williams and Wilkins",
number = "2",

}

TY - JOUR

T1 - Human circulating peripheral blood mononuclear cells for calvarial bone tissue engineering

AU - Chim, Harvey

AU - Schantz, Jan Thorsten

PY - 2006/2

Y1 - 2006/2

N2 - BACKGROUND: Large-scale production of engineered tissues requires an adequate source of expandable cells. Current strategies that involve harvesting of cells from donor tissue or bone marrow for tissue engineering are invasive and unfeasible for obtaining large quantities of cells in a clinical setting. Peripheral blood has been reported to contain circulating hematopoietic cells as well as, in significantly smaller quantities, mesenchymal cells METHODS: An adherent subset of CD14 mononuclear cells was isolated from human peripheral venous blood and characterized in vitro by light microscopy, immunohistochemistry, flow cytometry, and quantitative differentiation assays. These cells were then evaluated for the purposes of tissue engineering in a rat calvarial defect model, in combination with biodegradable polymer matrices made from poly-e-caprolactone. Specimens were analyzed 6 weeks after implantation with histologic analysis, microcomputed tomography, and HLA immunostaining. RESULTS: CD14 mononuclear cells were induced to differentiate into osteoblast-like cells in vitro, with areas of mineralization. In a rat calvarial defect model, tissue-engineered bone with evidence of mineralization was formed within 6 weeks. HLA immunohistochemistry demonstrated that de novo bone formation originated from the transplanted human cells. CONCLUSIONS: These findings show, for the first time, to our knowledge, the derivation of bone from human blood. They also demonstrate the utility of circulating mononuclear cells as a minimally invasive, potentially unlimited pool of cells for tissue engineering and organ regeneration.

AB - BACKGROUND: Large-scale production of engineered tissues requires an adequate source of expandable cells. Current strategies that involve harvesting of cells from donor tissue or bone marrow for tissue engineering are invasive and unfeasible for obtaining large quantities of cells in a clinical setting. Peripheral blood has been reported to contain circulating hematopoietic cells as well as, in significantly smaller quantities, mesenchymal cells METHODS: An adherent subset of CD14 mononuclear cells was isolated from human peripheral venous blood and characterized in vitro by light microscopy, immunohistochemistry, flow cytometry, and quantitative differentiation assays. These cells were then evaluated for the purposes of tissue engineering in a rat calvarial defect model, in combination with biodegradable polymer matrices made from poly-e-caprolactone. Specimens were analyzed 6 weeks after implantation with histologic analysis, microcomputed tomography, and HLA immunostaining. RESULTS: CD14 mononuclear cells were induced to differentiate into osteoblast-like cells in vitro, with areas of mineralization. In a rat calvarial defect model, tissue-engineered bone with evidence of mineralization was formed within 6 weeks. HLA immunohistochemistry demonstrated that de novo bone formation originated from the transplanted human cells. CONCLUSIONS: These findings show, for the first time, to our knowledge, the derivation of bone from human blood. They also demonstrate the utility of circulating mononuclear cells as a minimally invasive, potentially unlimited pool of cells for tissue engineering and organ regeneration.

UR - http://www.scopus.com/inward/record.url?scp=33644859570&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33644859570&partnerID=8YFLogxK

U2 - 10.1097/01.prs.0000201489.65811.e7

DO - 10.1097/01.prs.0000201489.65811.e7

M3 - Article

VL - 117

SP - 468

EP - 478

JO - Plastic and Reconstructive Surgery

JF - Plastic and Reconstructive Surgery

SN - 0032-1052

IS - 2

ER -