TY - JOUR
T1 - Human Bone Morphogenetic Protein (hBMP)
AU - Urist, Marshall R.
AU - Sato, Keiji
AU - Brownell, Anna G.
AU - Malinin, Theodore I.
AU - Lietze, Arthur
AU - Huo, Yong Kang
AU - Prolo, Donald J.
AU - Oklund, Sally
AU - Finerman, Gerald A.M.
AU - Delange, Robert J.
N1 - Funding Information:
These investigations were supported by grants-in-aid from USPHS, NIDR (DEO2103), The Kroc Foundation, and the Solo Cup Foundation. 6 1983 173 2 194 199
PY - 1983/6
Y1 - 1983/6
N2 - Human bone morphogenetic protein (hBMP) was chemically extracted from demineralized gelatinized cortical bone matrix by means of a CaCl2.urea inorganic-organic solvent mixture, differential precipitation in guanidine hydrochloride, and preparative gel electrophoresis. hBMP is isolated in quantities of 1 mg/kg of wet weight of fresh bone, and has the amino-acid composition of an acidic polypeptide. The mol wt is 17 to 18 k-DA (kilodaltons). Implants of the isolated 17-kDa protein are very rapidly adsorbed and produce a smaller volume of bone than protein fractions consisting of 24-, 17-, and 14-kDa proteins. Since the isolated 24- and 14-kDa components lack hBMP activity, the kinetics of the bone morphogenetic processes including the function of other proteins as carrier molecules, await investigation.
AB - Human bone morphogenetic protein (hBMP) was chemically extracted from demineralized gelatinized cortical bone matrix by means of a CaCl2.urea inorganic-organic solvent mixture, differential precipitation in guanidine hydrochloride, and preparative gel electrophoresis. hBMP is isolated in quantities of 1 mg/kg of wet weight of fresh bone, and has the amino-acid composition of an acidic polypeptide. The mol wt is 17 to 18 k-DA (kilodaltons). Implants of the isolated 17-kDa protein are very rapidly adsorbed and produce a smaller volume of bone than protein fractions consisting of 24-, 17-, and 14-kDa proteins. Since the isolated 24- and 14-kDa components lack hBMP activity, the kinetics of the bone morphogenetic processes including the function of other proteins as carrier molecules, await investigation.
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U2 - 10.3181/00379727-173-41630
DO - 10.3181/00379727-173-41630
M3 - Article
C2 - 6866999
AN - SCOPUS:0020630762
VL - 173
SP - 194
EP - 199
JO - Experimental Biology and Medicine
JF - Experimental Biology and Medicine
SN - 0037-9727
IS - 2
ER -