Human bone morphogenetic protein (hBMP)

M. R. Urist, K. Sato, A. G. Brownell, Theodore Malinin, A. Lietze, Y. K. Huo, D. J. Prolo, S. Oklund, G. A. Finerman, R. J. DeLange

Research output: Contribution to journalArticle

138 Citations (Scopus)

Abstract

Human bone morphogenetic protein (hBMP) was chemically extracted from demineralized gelatinized cortical bone matrix by means of a CaCl2.urea inorganic-organic solvent mixture, differential precipitation in guanidine hydrochloride, and preparative gel electrophoresis. hBMP is isolated in quantities of 1 mg/kg of wet weight of fresh bone, and has the amino-acid composition of an acidic polypeptide. The mol wt is 17 to 18 k-DA (kilodaltons). Implants of the isolated 17-kDa protein are very rapidly adsorbed and produce a smaller volume of bone than protein fractions consisting of 24-, 17-, and 14-kDa proteins. Since the isolated 24- and 14-kDa components lack hBMP activity, the kinetics of the bone morphogenetic processes including the function of other proteins as carrier molecules, await investigation.

Original languageEnglish
Pages (from-to)194-199
Number of pages6
JournalProceedings of the Society for Experimental Biology and Medicine
Volume173
Issue number2
StatePublished - Aug 4 1983
Externally publishedYes

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Bone Morphogenetic Proteins
Bone
Bone and Bones
Acidic Amino Acids
Bone Matrix
Proteins
Guanidine
Urea
Electrophoresis
Carrier Proteins
Gels
Organic solvents
Weights and Measures
Peptides
Amino Acids
Molecules
Kinetics
Chemical analysis

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Urist, M. R., Sato, K., Brownell, A. G., Malinin, T., Lietze, A., Huo, Y. K., ... DeLange, R. J. (1983). Human bone morphogenetic protein (hBMP). Proceedings of the Society for Experimental Biology and Medicine, 173(2), 194-199.

Human bone morphogenetic protein (hBMP). / Urist, M. R.; Sato, K.; Brownell, A. G.; Malinin, Theodore; Lietze, A.; Huo, Y. K.; Prolo, D. J.; Oklund, S.; Finerman, G. A.; DeLange, R. J.

In: Proceedings of the Society for Experimental Biology and Medicine, Vol. 173, No. 2, 04.08.1983, p. 194-199.

Research output: Contribution to journalArticle

Urist, MR, Sato, K, Brownell, AG, Malinin, T, Lietze, A, Huo, YK, Prolo, DJ, Oklund, S, Finerman, GA & DeLange, RJ 1983, 'Human bone morphogenetic protein (hBMP)', Proceedings of the Society for Experimental Biology and Medicine, vol. 173, no. 2, pp. 194-199.
Urist MR, Sato K, Brownell AG, Malinin T, Lietze A, Huo YK et al. Human bone morphogenetic protein (hBMP). Proceedings of the Society for Experimental Biology and Medicine. 1983 Aug 4;173(2):194-199.
Urist, M. R. ; Sato, K. ; Brownell, A. G. ; Malinin, Theodore ; Lietze, A. ; Huo, Y. K. ; Prolo, D. J. ; Oklund, S. ; Finerman, G. A. ; DeLange, R. J. / Human bone morphogenetic protein (hBMP). In: Proceedings of the Society for Experimental Biology and Medicine. 1983 ; Vol. 173, No. 2. pp. 194-199.
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