Abstract
The apolipoprotein E (ApoE) ε4 allele is a major risk factor for neurodegenerative conditions, including Alzheimer's disease. A role for ApoE is implicated in regeneration of synaptic circuitry after neural injury. In the in vitro mouse organotypic hippocampal slice culture system, we previously showed that cultures derived from ApoE-knockout mice are defective in mossy fiber sprouting into the dentate gyrus molecular layer. This sprouting defect was rescued in cultures from transgenic mice expressing ApoE3 under the control of the human promoter and in ApoE-knockout cultures treated with ApoE3-conditioned media. Although the ApoE3 transgene fully restored sprouting, ApoE4 restored sprouting to only 58% of ApoE3 levels. These data indicate that ApoE isoform-specific effects on neuroregeneration may contribute to its genetic risk for Alzheimer's disease.
| Original language | English |
|---|---|
| Pages (from-to) | 2613-2616 |
| Number of pages | 4 |
| Journal | Journal of Neurochemistry |
| Volume | 73 |
| Issue number | 6 |
| DOIs | |
| State | Published - Nov 29 1999 |
| Externally published | Yes |
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Keywords
- Alzheimer's disease
- Apolipoprotein E
- Neurite sprouting
- Transgenic mice
ASJC Scopus subject areas
- Biochemistry
- Cellular and Molecular Neuroscience
Cite this
Human apolipoprotein E isoform-specific differences in neuronal sprouting in organotypic hippocampal culture. / Teter, B.; Xu, P. T.; Gilbert, John; Roses, A. D.; Galasko, D.; Cole, G. M.
In: Journal of Neurochemistry, Vol. 73, No. 6, 29.11.1999, p. 2613-2616.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Human apolipoprotein E isoform-specific differences in neuronal sprouting in organotypic hippocampal culture
AU - Teter, B.
AU - Xu, P. T.
AU - Gilbert, John
AU - Roses, A. D.
AU - Galasko, D.
AU - Cole, G. M.
PY - 1999/11/29
Y1 - 1999/11/29
N2 - The apolipoprotein E (ApoE) ε4 allele is a major risk factor for neurodegenerative conditions, including Alzheimer's disease. A role for ApoE is implicated in regeneration of synaptic circuitry after neural injury. In the in vitro mouse organotypic hippocampal slice culture system, we previously showed that cultures derived from ApoE-knockout mice are defective in mossy fiber sprouting into the dentate gyrus molecular layer. This sprouting defect was rescued in cultures from transgenic mice expressing ApoE3 under the control of the human promoter and in ApoE-knockout cultures treated with ApoE3-conditioned media. Although the ApoE3 transgene fully restored sprouting, ApoE4 restored sprouting to only 58% of ApoE3 levels. These data indicate that ApoE isoform-specific effects on neuroregeneration may contribute to its genetic risk for Alzheimer's disease.
AB - The apolipoprotein E (ApoE) ε4 allele is a major risk factor for neurodegenerative conditions, including Alzheimer's disease. A role for ApoE is implicated in regeneration of synaptic circuitry after neural injury. In the in vitro mouse organotypic hippocampal slice culture system, we previously showed that cultures derived from ApoE-knockout mice are defective in mossy fiber sprouting into the dentate gyrus molecular layer. This sprouting defect was rescued in cultures from transgenic mice expressing ApoE3 under the control of the human promoter and in ApoE-knockout cultures treated with ApoE3-conditioned media. Although the ApoE3 transgene fully restored sprouting, ApoE4 restored sprouting to only 58% of ApoE3 levels. These data indicate that ApoE isoform-specific effects on neuroregeneration may contribute to its genetic risk for Alzheimer's disease.
KW - Alzheimer's disease
KW - Apolipoprotein E
KW - Neurite sprouting
KW - Transgenic mice
UR - http://www.scopus.com/inward/record.url?scp=0032708306&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032708306&partnerID=8YFLogxK
U2 - 10.1046/j.1471-4159.1999.0732613.x
DO - 10.1046/j.1471-4159.1999.0732613.x
M3 - Article
VL - 73
SP - 2613
EP - 2616
JO - Journal of Neurochemistry
JF - Journal of Neurochemistry
SN - 0022-3042
IS - 6
ER -