Human apolipoprotein E isoform-specific differences in neuronal sprouting in organotypic hippocampal culture

B. Teter, P. T. Xu, J. R. Gilbert, A. D. Roses, D. Galasko, G. M. Cole

Research output: Contribution to journalArticle

73 Scopus citations

Abstract

The apolipoprotein E (ApoE) ε4 allele is a major risk factor for neurodegenerative conditions, including Alzheimer's disease. A role for ApoE is implicated in regeneration of synaptic circuitry after neural injury. In the in vitro mouse organotypic hippocampal slice culture system, we previously showed that cultures derived from ApoE-knockout mice are defective in mossy fiber sprouting into the dentate gyrus molecular layer. This sprouting defect was rescued in cultures from transgenic mice expressing ApoE3 under the control of the human promoter and in ApoE-knockout cultures treated with ApoE3-conditioned media. Although the ApoE3 transgene fully restored sprouting, ApoE4 restored sprouting to only 58% of ApoE3 levels. These data indicate that ApoE isoform-specific effects on neuroregeneration may contribute to its genetic risk for Alzheimer's disease.

Original languageEnglish (US)
Pages (from-to)2613-2616
Number of pages4
JournalJournal of neurochemistry
Volume73
Issue number6
DOIs
StatePublished - Nov 29 1999

Keywords

  • Alzheimer's disease
  • Apolipoprotein E
  • Neurite sprouting
  • Transgenic mice

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Fingerprint Dive into the research topics of 'Human apolipoprotein E isoform-specific differences in neuronal sprouting in organotypic hippocampal culture'. Together they form a unique fingerprint.

  • Cite this