Human amniotic fluid stem cells can integrate and differentiate into epithelial lung lineages

Gianni Carraro, Laura Perin, Sargis Sedrakyan, Stefano Giuliani, Caterina Tiozzo, Jooeun Lee, Gianluca Turcatel, Stijn P. De Langhe, Barbara Driscoll, Saverio Bellusci, Parviz Minoo, Anthony Atala, Roger Edward De Filippo, David Warburton

Research output: Contribution to journalArticlepeer-review

184 Scopus citations


A new source of stem cells has recently been isolated from amniotic fluid; these amniotic fluid stem cells have significant potential for regenerative medicine. These cells are multipotent, showing the ability to differentiate into cell types from each embryonic germ layer. We investigated the ability of human amniotic fluid stem cells (hAFSC) to integrate into murine lung and to differentiate into pulmonary lineages after injury. Using microinjection into cultured mouse embryonic lungs, hAFSC can integrate into the epithelium and express the early human differentiation marker thyroid transcription factor 1 (TTF1). In adult nude mice, following hyperoxia injury, tail vein-injected hAFSC localized in the distal lung and expressed both TTF1 and the type II pneumocyte marker surfactant protein C. Specific damage of Clara cells through naphthalene injury produced integration and differentiation of hAFSC at the bronchioalveolar and bronchial positions with expression of the specific Clara cell 10-kDa protein. These results illustrate the plasticity of hAFSC to respond in different ways to different types of lung damage by expressing specific alveolar versus bronchiolar epithelial cell lineage markers, depending on the type of injury to recipient lung.

Original languageEnglish (US)
Pages (from-to)2902-2911
Number of pages10
Issue number11
StatePublished - Nov 2008


  • Amniotic fluid stem cells
  • Lung injury
  • Regenerative medicine
  • Tissue repair

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Molecular Medicine


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