Human amniotic fluid-derived stem cells are rejected after transplantation in the myocardium of normal, ischemic, immuno-suppressed or immuno-deficient rat

Angela Chiavegato, Sveva Bollini, Michela Pozzobon, Andrea Callegari, Lisa Gasparotto, Jenny Taiani, Martina Piccoli, Elisabetta Lenzini, Gino Gerosa, Igor Vendramin, Emanuele Cozzi, Annalisa Angelini, Laura Iop, Giovanni F. Zanon, Anthony Atala, Paolo De Coppi, Saverio Sartore

Research output: Contribution to journalArticle

124 Citations (Scopus)

Abstract

Human amniotic fluid-derived stem (AFS) cells, similarly to embryonic stem cells, could possess privileged immunological characteristics suitable for a successful transplantation even in a discordant xenograft system. We investigated whether AFS cells could be fruitfully used in a rat model of myocardial infarction. c-kit immunomagnetic-sorted AFS cells were characterized by flow cytometric analysis and cytospins as well as reverse-transcription polymerase chain reaction, Western blotting and immunocytochemistry for cardiovascular differentiation markers. In vitro, AFS cell phenotypic conversion was assayed by cardiovascular-specific induction media or co-cultured with rat neonatal cardiomyocytes. AFS cells showed mRNAs and/or protein for endothelial (angiopoietin, CD146) and smooth muscle (smoothelin) cells, and cardiomyocyte (Nkx2.5, MLC-2v, GATA-4, β-MyHC) markers. Acquisition of a cardiomyocyte-like phenotype in rare AFS cells could be seen only in co-cultures with rat neonatal cells. In vivo, AFS cells xenotransplantated in a rat model of myocardial infarction, with or without cyclosporine treatment, or in intact heart from immuno-competent or immuno-deficient animals were acutely rejected due to the different recruitment of recipient CD4+, CD8+ T and B lymphocytes, NK cells and macrophages. This reaction is most likely to be linked to expression of B7 co-stimulatory molecules CD80 and CD86 as well as macrophage marker CD68 on AFS cells. Xenotransplanted AFS cells gave also rise in some animals to cell masses in the subendocardium and myocardium suggestive of a process of chondro-osteogenic differentiation. Despite AFS cells in vitro can differentiate to some extent to cells of cardiovascular lineages, their in vivo use in xenotransplantation for cell therapy of myocardial infarction is hampered by their peculiar immunogenic properties and phenotypic instability.

Original languageEnglish
Pages (from-to)746-759
Number of pages14
JournalJournal of Molecular and Cellular Cardiology
Volume42
Issue number4
DOIs
StatePublished - Apr 1 2007
Externally publishedYes

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Amniotic Fluid
Myocardium
Stem Cells
Transplantation
Cardiac Myocytes
Myocardial Infarction
Macrophages
Angiopoietins
Heterologous Transplantation
Differentiation Antigens
Cell Lineage
Embryonic Stem Cells
Cell- and Tissue-Based Therapy
Coculture Techniques
Heterografts
Natural Killer Cells
Cyclosporine
Reverse Transcription
Smooth Muscle Myocytes
B-Lymphocytes

Keywords

  • Cell therapy
  • Immune rejection
  • Myocardial ischemia
  • Stem cells
  • Xenotransplantation

ASJC Scopus subject areas

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

Cite this

Human amniotic fluid-derived stem cells are rejected after transplantation in the myocardium of normal, ischemic, immuno-suppressed or immuno-deficient rat. / Chiavegato, Angela; Bollini, Sveva; Pozzobon, Michela; Callegari, Andrea; Gasparotto, Lisa; Taiani, Jenny; Piccoli, Martina; Lenzini, Elisabetta; Gerosa, Gino; Vendramin, Igor; Cozzi, Emanuele; Angelini, Annalisa; Iop, Laura; Zanon, Giovanni F.; Atala, Anthony; De Coppi, Paolo; Sartore, Saverio.

In: Journal of Molecular and Cellular Cardiology, Vol. 42, No. 4, 01.04.2007, p. 746-759.

Research output: Contribution to journalArticle

Chiavegato, A, Bollini, S, Pozzobon, M, Callegari, A, Gasparotto, L, Taiani, J, Piccoli, M, Lenzini, E, Gerosa, G, Vendramin, I, Cozzi, E, Angelini, A, Iop, L, Zanon, GF, Atala, A, De Coppi, P & Sartore, S 2007, 'Human amniotic fluid-derived stem cells are rejected after transplantation in the myocardium of normal, ischemic, immuno-suppressed or immuno-deficient rat', Journal of Molecular and Cellular Cardiology, vol. 42, no. 4, pp. 746-759. https://doi.org/10.1016/j.yjmcc.2006.12.008
Chiavegato, Angela ; Bollini, Sveva ; Pozzobon, Michela ; Callegari, Andrea ; Gasparotto, Lisa ; Taiani, Jenny ; Piccoli, Martina ; Lenzini, Elisabetta ; Gerosa, Gino ; Vendramin, Igor ; Cozzi, Emanuele ; Angelini, Annalisa ; Iop, Laura ; Zanon, Giovanni F. ; Atala, Anthony ; De Coppi, Paolo ; Sartore, Saverio. / Human amniotic fluid-derived stem cells are rejected after transplantation in the myocardium of normal, ischemic, immuno-suppressed or immuno-deficient rat. In: Journal of Molecular and Cellular Cardiology. 2007 ; Vol. 42, No. 4. pp. 746-759.
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AU - Callegari, Andrea

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AU - Taiani, Jenny

AU - Piccoli, Martina

AU - Lenzini, Elisabetta

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AU - Vendramin, Igor

AU - Cozzi, Emanuele

AU - Angelini, Annalisa

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