Human albumin solder supplemented with TGF-β1 accelerates healing following laser welded wound closure

Dix P. Poppas, J. Mathieu Massicotte, Robert B. Stewart, Anita B. Roberts, Anthony Atala, Alan B. Retik, Michael R. Freeman

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Background and Objective: We examined the possibility that human albumin solder can be used as a vehicle for site specific delivery of growth factors for the purpose of accelerating tissue repair following laser welded wound closure. Certain human recombinant growth factors have been shown to accelerate wound healing in model systems. Pilot in vitro studies have established that several growth factors, including TGF-β1, maintain bioactivity following exposure to temperatures achieved during laser tissue welding. Using a temperature controlled laser delivery system (TCL) to precisely maintain welding temperatures, it is now possible to avoid thermal denaturation of exogenous bioactive molecules such as growth factors. Study Design/Materials and Methods: HB-EGF, bFGF, and TGF-β1 were tested in vitro for maintenance of bioactivity after exposure to 80°C. In vivo experiments using porcine skin determined the efficacy of solders augmented with growth factors. Incisions were repaired using human albumin alone or supplemented with HB-EGF (2 μg), bFGF (10 μg), or TGF-β1 (1 μg). Wounds were excised at 3, 5, and 7 days post-operatively. Tensile strength, total collagen content, and histology were performed. Results: At 3 days, tensile strength (TS) of TGF-β1 wounds were 36% (P < 0.05) and 20% (n.s.) stronger than laser alone and suture closures, respectively. By 5 days the TS of the TGF- β1 group increased by 50% (P < 0.05) and 59% (P < 0.02) over laser alone and suture groups, respectively. At 7 days the TGF-β1 group was 50% (P < 0.05) and 79% (P < 0.01) stronger than laser solder alone or suture, respectively. The HB-EGF and bFGF groups were equivalent to the laser solder group at all time points. Total collagen content at 7 days increased in the TGF-β1 group by 7% (n.s.) over the suture group and 21% (P < 0.05) in the laser group. Conclusion: Human albumin solder supplemented with TGF-β1 increases the early post-operative strength of laser welded wounds. This novel application of laser tissue soldering augmented with a growth factor has the potential to bring about immediate fluid tight seals while providing site specific delivery of biological modifiers. This may lead to an overall improvement in post-operative convalescence, wound infections, and hospital costs.

Original languageEnglish
Pages (from-to)360-368
Number of pages9
JournalLasers in Surgery and Medicine
Volume19
Issue number3
DOIs
StatePublished - Nov 19 1996
Externally publishedYes

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Albumins
Lasers
Wounds and Injuries
Intercellular Signaling Peptides and Proteins
Sutures
Tensile Strength
Welding
Temperature
Collagen
Hospital Costs
Wound Infection
Wound Healing
Histology
Swine
Hot Temperature
Maintenance
Skin
Heparin-binding EGF-like Growth Factor

ASJC Scopus subject areas

  • Surgery

Cite this

Human albumin solder supplemented with TGF-β1 accelerates healing following laser welded wound closure. / Poppas, Dix P.; Massicotte, J. Mathieu; Stewart, Robert B.; Roberts, Anita B.; Atala, Anthony; Retik, Alan B.; Freeman, Michael R.

In: Lasers in Surgery and Medicine, Vol. 19, No. 3, 19.11.1996, p. 360-368.

Research output: Contribution to journalArticle

Poppas, Dix P. ; Massicotte, J. Mathieu ; Stewart, Robert B. ; Roberts, Anita B. ; Atala, Anthony ; Retik, Alan B. ; Freeman, Michael R. / Human albumin solder supplemented with TGF-β1 accelerates healing following laser welded wound closure. In: Lasers in Surgery and Medicine. 1996 ; Vol. 19, No. 3. pp. 360-368.
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abstract = "Background and Objective: We examined the possibility that human albumin solder can be used as a vehicle for site specific delivery of growth factors for the purpose of accelerating tissue repair following laser welded wound closure. Certain human recombinant growth factors have been shown to accelerate wound healing in model systems. Pilot in vitro studies have established that several growth factors, including TGF-β1, maintain bioactivity following exposure to temperatures achieved during laser tissue welding. Using a temperature controlled laser delivery system (TCL) to precisely maintain welding temperatures, it is now possible to avoid thermal denaturation of exogenous bioactive molecules such as growth factors. Study Design/Materials and Methods: HB-EGF, bFGF, and TGF-β1 were tested in vitro for maintenance of bioactivity after exposure to 80°C. In vivo experiments using porcine skin determined the efficacy of solders augmented with growth factors. Incisions were repaired using human albumin alone or supplemented with HB-EGF (2 μg), bFGF (10 μg), or TGF-β1 (1 μg). Wounds were excised at 3, 5, and 7 days post-operatively. Tensile strength, total collagen content, and histology were performed. Results: At 3 days, tensile strength (TS) of TGF-β1 wounds were 36{\%} (P < 0.05) and 20{\%} (n.s.) stronger than laser alone and suture closures, respectively. By 5 days the TS of the TGF- β1 group increased by 50{\%} (P < 0.05) and 59{\%} (P < 0.02) over laser alone and suture groups, respectively. At 7 days the TGF-β1 group was 50{\%} (P < 0.05) and 79{\%} (P < 0.01) stronger than laser solder alone or suture, respectively. The HB-EGF and bFGF groups were equivalent to the laser solder group at all time points. Total collagen content at 7 days increased in the TGF-β1 group by 7{\%} (n.s.) over the suture group and 21{\%} (P < 0.05) in the laser group. Conclusion: Human albumin solder supplemented with TGF-β1 increases the early post-operative strength of laser welded wounds. This novel application of laser tissue soldering augmented with a growth factor has the potential to bring about immediate fluid tight seals while providing site specific delivery of biological modifiers. This may lead to an overall improvement in post-operative convalescence, wound infections, and hospital costs.",
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AU - Stewart, Robert B.

AU - Roberts, Anita B.

AU - Atala, Anthony

AU - Retik, Alan B.

AU - Freeman, Michael R.

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N2 - Background and Objective: We examined the possibility that human albumin solder can be used as a vehicle for site specific delivery of growth factors for the purpose of accelerating tissue repair following laser welded wound closure. Certain human recombinant growth factors have been shown to accelerate wound healing in model systems. Pilot in vitro studies have established that several growth factors, including TGF-β1, maintain bioactivity following exposure to temperatures achieved during laser tissue welding. Using a temperature controlled laser delivery system (TCL) to precisely maintain welding temperatures, it is now possible to avoid thermal denaturation of exogenous bioactive molecules such as growth factors. Study Design/Materials and Methods: HB-EGF, bFGF, and TGF-β1 were tested in vitro for maintenance of bioactivity after exposure to 80°C. In vivo experiments using porcine skin determined the efficacy of solders augmented with growth factors. Incisions were repaired using human albumin alone or supplemented with HB-EGF (2 μg), bFGF (10 μg), or TGF-β1 (1 μg). Wounds were excised at 3, 5, and 7 days post-operatively. Tensile strength, total collagen content, and histology were performed. Results: At 3 days, tensile strength (TS) of TGF-β1 wounds were 36% (P < 0.05) and 20% (n.s.) stronger than laser alone and suture closures, respectively. By 5 days the TS of the TGF- β1 group increased by 50% (P < 0.05) and 59% (P < 0.02) over laser alone and suture groups, respectively. At 7 days the TGF-β1 group was 50% (P < 0.05) and 79% (P < 0.01) stronger than laser solder alone or suture, respectively. The HB-EGF and bFGF groups were equivalent to the laser solder group at all time points. Total collagen content at 7 days increased in the TGF-β1 group by 7% (n.s.) over the suture group and 21% (P < 0.05) in the laser group. Conclusion: Human albumin solder supplemented with TGF-β1 increases the early post-operative strength of laser welded wounds. This novel application of laser tissue soldering augmented with a growth factor has the potential to bring about immediate fluid tight seals while providing site specific delivery of biological modifiers. This may lead to an overall improvement in post-operative convalescence, wound infections, and hospital costs.

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