HSV-mediated transfer of interleukin-10 reduces inflammatory pain through modulation of membrane tumor necrosis factor α in spinal cord microglia

Z. Zhou, X. Peng, S. Hao, D. J. Fink, M. Mata

Research output: Contribution to journalArticle

64 Scopus citations

Abstract

To dissect the molecular basis of the neuroimmune response associated with the genesis of inflammatory (nociceptive) pain, we constructed a herpes simplex virus-based gene transfer vector to express the antiinflammatory cytokine interleukin-10 (IL-10), and used it to examine the effect of IL-10 expression in activated microglial cells in vitro, and in inflammatory pain in vivo. IL-10 reduced the phosphorylation of p38 mitogen-activated protein kinase (MAPK) and decreased the expression of full-length membrane spanning tumor necrosis factor-α (mTNFα) following lipopolysaccharide stimulation of microglia in vitro. IL-10 also reduced intracellular cleavage of mTNFα and release of the soluble cleavage product sTNFα. Similar effects on TNFα expression were observed when the cells were pretreated with a p38 MAPK inhibitor. In animals, injection of a dilute solution of formalin in the skin resulted in an increase in mTNFα in spinal dorsal horn, without detectable sTNFα. Local release of IL-10 achieved by gene transfer reduced the number of spontaneous flinches in the early and delayed phases of the formalin test of inflammatory pain. The effect of IL-10 on nocisponsive behavior correlated with a block in phosphorylation of p38 and reduced expression of 26kDa mTNFα in spinal microglia. The results emphasize the key role played by membrane TNFα in the spinal neuroimmune response in pain caused by peripheral inflammation.

Original languageEnglish (US)
Pages (from-to)183-190
Number of pages8
JournalGene Therapy
Volume15
Issue number3
DOIs
StatePublished - Feb 1 2008
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Genetics

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