HSD18B7 enzyme assay technique using a triple quadrupole mass spectrometer

Carina Wu, Nhuong Sao Ton, Maria Fernanda Suarez, Maria Piqueras, Sanjoy K. Bhattacharya

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Enzymes exist in all biological systems to catalyze vital biochemical reactions. The reactivity of an enzyme and the extent of its influence on product formation can give insight to understanding the physiological changes that can take place. The enzyme HSD17B7, involved in the cholesterol biosynthesis pathway, may play a role in influencing underlying changes during transition of disease, specifically in eyes at normal state to eyes that have glaucoma. In this work, we present a method to test the enzymatic activity level of HSD17B7 between normal and glaucomatous optic nerves to assess whether enzymatic upregulation of cholesterol biosynthesis may play a role in glaucoma.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages155-159
Number of pages5
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1996
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Biosynthesis pathway
  • Catalyze
  • Cholesterol
  • Dihydrotestosterone (DHT)
  • HSD17B7 enzyme
  • Substrate
  • Triple quadrupole mass spectrometer

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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