HSD18B7 enzyme assay technique using a triple quadrupole mass spectrometer

Carina Wu, Nhuong Sao Ton, Maria Fernanda Suarez, Maria Piqueras, Sanjoy K Bhattacharya

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Enzymes exist in all biological systems to catalyze vital biochemical reactions. The reactivity of an enzyme and the extent of its influence on product formation can give insight to understanding the physiological changes that can take place. The enzyme HSD17B7, involved in the cholesterol biosynthesis pathway, may play a role in influencing underlying changes during transition of disease, specifically in eyes at normal state to eyes that have glaucoma. In this work, we present a method to test the enzymatic activity level of HSD17B7 between normal and glaucomatous optic nerves to assess whether enzymatic upregulation of cholesterol biosynthesis may play a role in glaucoma.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages155-159
Number of pages5
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1996
ISSN (Print)1064-3745

Fingerprint

Enzyme Assays
Glaucoma
Enzymes
Cholesterol
Optic Nerve
Up-Regulation

Keywords

  • Biosynthesis pathway
  • Catalyze
  • Cholesterol
  • Dihydrotestosterone (DHT)
  • HSD17B7 enzyme
  • Substrate
  • Triple quadrupole mass spectrometer

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Wu, C., Ton, N. S., Suarez, M. F., Piqueras, M., & Bhattacharya, S. K. (2019). HSD18B7 enzyme assay technique using a triple quadrupole mass spectrometer. In Methods in Molecular Biology (pp. 155-159). (Methods in Molecular Biology; Vol. 1996). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9488-5_13

HSD18B7 enzyme assay technique using a triple quadrupole mass spectrometer. / Wu, Carina; Ton, Nhuong Sao; Suarez, Maria Fernanda; Piqueras, Maria; Bhattacharya, Sanjoy K.

Methods in Molecular Biology. Humana Press Inc., 2019. p. 155-159 (Methods in Molecular Biology; Vol. 1996).

Research output: Chapter in Book/Report/Conference proceedingChapter

Wu, C, Ton, NS, Suarez, MF, Piqueras, M & Bhattacharya, SK 2019, HSD18B7 enzyme assay technique using a triple quadrupole mass spectrometer. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1996, Humana Press Inc., pp. 155-159. https://doi.org/10.1007/978-1-4939-9488-5_13
Wu C, Ton NS, Suarez MF, Piqueras M, Bhattacharya SK. HSD18B7 enzyme assay technique using a triple quadrupole mass spectrometer. In Methods in Molecular Biology. Humana Press Inc. 2019. p. 155-159. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-9488-5_13
Wu, Carina ; Ton, Nhuong Sao ; Suarez, Maria Fernanda ; Piqueras, Maria ; Bhattacharya, Sanjoy K. / HSD18B7 enzyme assay technique using a triple quadrupole mass spectrometer. Methods in Molecular Biology. Humana Press Inc., 2019. pp. 155-159 (Methods in Molecular Biology).
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