HPV in HIV-infected women: Implications for primary prevention

Nathalie Dauphin McKenzie, Erin Kobetz, Parvin Ganjei-Azar, Isabella Rosa-Cunha, JoNell E Potter, Atsushi Morishita, Joseph A. Lucci, Toumy Guettouche, James H. Hnatyszyn, Tulay Sengul

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Background: There is growing evidence that human immunodeficiency virus (HIV)-infected women might have a different human papillomavirus (HPV) type distribution in cervical dysplasia specimens as compared to the general population. This has implications for primary prevention. Objective: We aimed to obtain preliminary data on the HPV genotypes prevalent in histological samples of HIV-infected women with cervical intraepithelial neoplasia (CIN) 3/CIS of the cervix in Miami, FL, USA. Methods: Retrospective data were collected on HIV-infected women referred to the University of Miami-Jackson Memorial Hospital colposcopy clinic between years 2000 and 2008. The histology slides of CIN 3/CIS biopsies underwent pathological review and sections were cut from these archived specimens for HPV DNA extraction. HPV genotyping was then performed using the GeneSquare™ HPV genotyping assay. We report on our first set of 23 samples. Results: Eight high-risk HPV types were detected. Types in decreasing order of frequency were 16, 35, 45, 52, 59, 31, 58, and 56. Most cases had multiple infections. HPV type 16 was the most common (45%) followed by HPV-35 and -45 with equal frequency (40%). No samples contained HPV-18. Conclusion: Our preliminary results suggest that cervical dysplasia specimens of HIV-infected women more likely (55%) contain non-16 and -18 high-risk HPV types. We show that this held true for histologically confirmed severe dysplasia and carcinoma-in situ. Epidemiological studies guide vaccine development, therefore HPV type prevalence in CIS and invasive cervical cancer among HIV-infected women should be more rigorously explored to ensure that this highly vulnerable population receives appropriate primary prevention.

Original languageEnglish
Article number179
JournalFrontiers in Oncology
Volume4 AUG
StatePublished - Jan 1 2014

Fingerprint

Primary Prevention
HIV
Uterine Cervical Dysplasia
Cervical Intraepithelial Neoplasia
Human papillomavirus 18
Colposcopy
Human papillomavirus 16
Carcinoma in Situ
Human Development
Vulnerable Populations
Cervix Uteri
Uterine Cervical Neoplasms
Epidemiologic Studies
Histology
Vaccines
Genotype
Biopsy
DNA
Infection
Population

Keywords

  • Cervical cancer
  • CIN
  • HIV
  • HPV

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

HPV in HIV-infected women : Implications for primary prevention. / McKenzie, Nathalie Dauphin; Kobetz, Erin; Ganjei-Azar, Parvin; Rosa-Cunha, Isabella; Potter, JoNell E; Morishita, Atsushi; Lucci, Joseph A.; Guettouche, Toumy; Hnatyszyn, James H.; Sengul, Tulay.

In: Frontiers in Oncology, Vol. 4 AUG, 179, 01.01.2014.

Research output: Contribution to journalArticle

McKenzie, ND, Kobetz, E, Ganjei-Azar, P, Rosa-Cunha, I, Potter, JE, Morishita, A, Lucci, JA, Guettouche, T, Hnatyszyn, JH & Sengul, T 2014, 'HPV in HIV-infected women: Implications for primary prevention', Frontiers in Oncology, vol. 4 AUG, 179.
McKenzie, Nathalie Dauphin ; Kobetz, Erin ; Ganjei-Azar, Parvin ; Rosa-Cunha, Isabella ; Potter, JoNell E ; Morishita, Atsushi ; Lucci, Joseph A. ; Guettouche, Toumy ; Hnatyszyn, James H. ; Sengul, Tulay. / HPV in HIV-infected women : Implications for primary prevention. In: Frontiers in Oncology. 2014 ; Vol. 4 AUG.
@article{80d50c7450164ce782d76472ad09bb31,
title = "HPV in HIV-infected women: Implications for primary prevention",
abstract = "Background: There is growing evidence that human immunodeficiency virus (HIV)-infected women might have a different human papillomavirus (HPV) type distribution in cervical dysplasia specimens as compared to the general population. This has implications for primary prevention. Objective: We aimed to obtain preliminary data on the HPV genotypes prevalent in histological samples of HIV-infected women with cervical intraepithelial neoplasia (CIN) 3/CIS of the cervix in Miami, FL, USA. Methods: Retrospective data were collected on HIV-infected women referred to the University of Miami-Jackson Memorial Hospital colposcopy clinic between years 2000 and 2008. The histology slides of CIN 3/CIS biopsies underwent pathological review and sections were cut from these archived specimens for HPV DNA extraction. HPV genotyping was then performed using the GeneSquare™ HPV genotyping assay. We report on our first set of 23 samples. Results: Eight high-risk HPV types were detected. Types in decreasing order of frequency were 16, 35, 45, 52, 59, 31, 58, and 56. Most cases had multiple infections. HPV type 16 was the most common (45{\%}) followed by HPV-35 and -45 with equal frequency (40{\%}). No samples contained HPV-18. Conclusion: Our preliminary results suggest that cervical dysplasia specimens of HIV-infected women more likely (55{\%}) contain non-16 and -18 high-risk HPV types. We show that this held true for histologically confirmed severe dysplasia and carcinoma-in situ. Epidemiological studies guide vaccine development, therefore HPV type prevalence in CIS and invasive cervical cancer among HIV-infected women should be more rigorously explored to ensure that this highly vulnerable population receives appropriate primary prevention.",
keywords = "Cervical cancer, CIN, HIV, HPV",
author = "McKenzie, {Nathalie Dauphin} and Erin Kobetz and Parvin Ganjei-Azar and Isabella Rosa-Cunha and Potter, {JoNell E} and Atsushi Morishita and Lucci, {Joseph A.} and Toumy Guettouche and Hnatyszyn, {James H.} and Tulay Sengul",
year = "2014",
month = "1",
day = "1",
language = "English",
volume = "4 AUG",
journal = "Frontiers in Oncology",
issn = "2234-943X",
publisher = "Frontiers Media S. A.",

}

TY - JOUR

T1 - HPV in HIV-infected women

T2 - Implications for primary prevention

AU - McKenzie, Nathalie Dauphin

AU - Kobetz, Erin

AU - Ganjei-Azar, Parvin

AU - Rosa-Cunha, Isabella

AU - Potter, JoNell E

AU - Morishita, Atsushi

AU - Lucci, Joseph A.

AU - Guettouche, Toumy

AU - Hnatyszyn, James H.

AU - Sengul, Tulay

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Background: There is growing evidence that human immunodeficiency virus (HIV)-infected women might have a different human papillomavirus (HPV) type distribution in cervical dysplasia specimens as compared to the general population. This has implications for primary prevention. Objective: We aimed to obtain preliminary data on the HPV genotypes prevalent in histological samples of HIV-infected women with cervical intraepithelial neoplasia (CIN) 3/CIS of the cervix in Miami, FL, USA. Methods: Retrospective data were collected on HIV-infected women referred to the University of Miami-Jackson Memorial Hospital colposcopy clinic between years 2000 and 2008. The histology slides of CIN 3/CIS biopsies underwent pathological review and sections were cut from these archived specimens for HPV DNA extraction. HPV genotyping was then performed using the GeneSquare™ HPV genotyping assay. We report on our first set of 23 samples. Results: Eight high-risk HPV types were detected. Types in decreasing order of frequency were 16, 35, 45, 52, 59, 31, 58, and 56. Most cases had multiple infections. HPV type 16 was the most common (45%) followed by HPV-35 and -45 with equal frequency (40%). No samples contained HPV-18. Conclusion: Our preliminary results suggest that cervical dysplasia specimens of HIV-infected women more likely (55%) contain non-16 and -18 high-risk HPV types. We show that this held true for histologically confirmed severe dysplasia and carcinoma-in situ. Epidemiological studies guide vaccine development, therefore HPV type prevalence in CIS and invasive cervical cancer among HIV-infected women should be more rigorously explored to ensure that this highly vulnerable population receives appropriate primary prevention.

AB - Background: There is growing evidence that human immunodeficiency virus (HIV)-infected women might have a different human papillomavirus (HPV) type distribution in cervical dysplasia specimens as compared to the general population. This has implications for primary prevention. Objective: We aimed to obtain preliminary data on the HPV genotypes prevalent in histological samples of HIV-infected women with cervical intraepithelial neoplasia (CIN) 3/CIS of the cervix in Miami, FL, USA. Methods: Retrospective data were collected on HIV-infected women referred to the University of Miami-Jackson Memorial Hospital colposcopy clinic between years 2000 and 2008. The histology slides of CIN 3/CIS biopsies underwent pathological review and sections were cut from these archived specimens for HPV DNA extraction. HPV genotyping was then performed using the GeneSquare™ HPV genotyping assay. We report on our first set of 23 samples. Results: Eight high-risk HPV types were detected. Types in decreasing order of frequency were 16, 35, 45, 52, 59, 31, 58, and 56. Most cases had multiple infections. HPV type 16 was the most common (45%) followed by HPV-35 and -45 with equal frequency (40%). No samples contained HPV-18. Conclusion: Our preliminary results suggest that cervical dysplasia specimens of HIV-infected women more likely (55%) contain non-16 and -18 high-risk HPV types. We show that this held true for histologically confirmed severe dysplasia and carcinoma-in situ. Epidemiological studies guide vaccine development, therefore HPV type prevalence in CIS and invasive cervical cancer among HIV-infected women should be more rigorously explored to ensure that this highly vulnerable population receives appropriate primary prevention.

KW - Cervical cancer

KW - CIN

KW - HIV

KW - HPV

UR - http://www.scopus.com/inward/record.url?scp=84906315421&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84906315421&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:84906315421

VL - 4 AUG

JO - Frontiers in Oncology

JF - Frontiers in Oncology

SN - 2234-943X

M1 - 179

ER -