HPLC separation of inositol polyphosphates.

Christopher J. Barker, Christopher Illies, Per Olof Berggren

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

High performance liquid chromatography (HPLC) is an essential analytical tool in the study of the large number of inositol phosphate isomers. This chapter focuses on the separation of inositol polyphosphates from [(3)H]myo-inositol labeled tissues and cells. We review the different HPLC columns that have been used to separate inositol phosphates and their advantages and disadvantages. We describe important elements of sample preparation for effective separations and give examples of how changing factors, such as pH, can considerably improve the resolving ability of the HPLC chromatogram.

Original languageEnglish (US)
Pages (from-to)21-46
Number of pages26
JournalMethods in molecular biology (Clifton, N.J.)
Volume645
DOIs
StatePublished - Nov 8 2010

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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