HPLC separation of inositol polyphosphates.

Christopher J. Barker, Christopher Illies, Per Olof Berggren

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

High performance liquid chromatography (HPLC) is an essential analytical tool in the study of the large number of inositol phosphate isomers. This chapter focuses on the separation of inositol polyphosphates from [(3)H]myo-inositol labeled tissues and cells. We review the different HPLC columns that have been used to separate inositol phosphates and their advantages and disadvantages. We describe important elements of sample preparation for effective separations and give examples of how changing factors, such as pH, can considerably improve the resolving ability of the HPLC chromatogram.

Original languageEnglish
Pages (from-to)21-46
Number of pages26
JournalMethods in molecular biology (Clifton, N.J.)
Volume645
DOIs
StatePublished - Nov 8 2010
Externally publishedYes

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Polyphosphates
Inositol
Inositol Phosphates
High Pressure Liquid Chromatography

ASJC Scopus subject areas

  • Medicine(all)

Cite this

HPLC separation of inositol polyphosphates. / Barker, Christopher J.; Illies, Christopher; Berggren, Per Olof.

In: Methods in molecular biology (Clifton, N.J.), Vol. 645, 08.11.2010, p. 21-46.

Research output: Contribution to journalArticle

Barker, Christopher J. ; Illies, Christopher ; Berggren, Per Olof. / HPLC separation of inositol polyphosphates. In: Methods in molecular biology (Clifton, N.J.). 2010 ; Vol. 645. pp. 21-46.
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