Abstract
Background: 3′-Terminal CCA-containing tRNAs and precursors are resistant to action of RNase BN/RNase Z. Results: Arg274 and the two C residues are required for protection by the CCA sequence. Conclusion: Presence of Arg274 and CC sequence prevents RNA substrate from moving into the RNase catalytic site. Significance: This mechanism explains how mature tRNAs are protected from removal of the CCA sequence by a processing RNase.
Original language | English (US) |
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Pages (from-to) | 30636-30644 |
Number of pages | 9 |
Journal | Journal of Biological Chemistry |
Volume | 288 |
Issue number | 42 |
DOIs | |
State | Published - Oct 18 2013 |
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology