Abstract
Background: 3′-Terminal CCA-containing tRNAs and precursors are resistant to action of RNase BN/RNase Z. Results: Arg274 and the two C residues are required for protection by the CCA sequence. Conclusion: Presence of Arg274 and CC sequence prevents RNA substrate from moving into the RNase catalytic site. Significance: This mechanism explains how mature tRNAs are protected from removal of the CCA sequence by a processing RNase.
| Original language | English |
|---|---|
| Pages (from-to) | 30636-30644 |
| Number of pages | 9 |
| Journal | Journal of Biological Chemistry |
| Volume | 288 |
| Issue number | 42 |
| DOIs | |
| State | Published - Oct 18 2013 |
Fingerprint
ASJC Scopus subject areas
- Biochemistry
- Cell Biology
- Molecular Biology
Cite this
How a CCA sequence protects mature tRNAs and tRNA precursors from action of the processing enzyme RNase BN/RNase Z. / Dutta, Tanmay; Malhotra, Arun; Deutscher, Murray P.
In: Journal of Biological Chemistry, Vol. 288, No. 42, 18.10.2013, p. 30636-30644.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - How a CCA sequence protects mature tRNAs and tRNA precursors from action of the processing enzyme RNase BN/RNase Z
AU - Dutta, Tanmay
AU - Malhotra, Arun
AU - Deutscher, Murray P
PY - 2013/10/18
Y1 - 2013/10/18
N2 - Background: 3′-Terminal CCA-containing tRNAs and precursors are resistant to action of RNase BN/RNase Z. Results: Arg274 and the two C residues are required for protection by the CCA sequence. Conclusion: Presence of Arg274 and CC sequence prevents RNA substrate from moving into the RNase catalytic site. Significance: This mechanism explains how mature tRNAs are protected from removal of the CCA sequence by a processing RNase.
AB - Background: 3′-Terminal CCA-containing tRNAs and precursors are resistant to action of RNase BN/RNase Z. Results: Arg274 and the two C residues are required for protection by the CCA sequence. Conclusion: Presence of Arg274 and CC sequence prevents RNA substrate from moving into the RNase catalytic site. Significance: This mechanism explains how mature tRNAs are protected from removal of the CCA sequence by a processing RNase.
UR - http://www.scopus.com/inward/record.url?scp=84886886742&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84886886742&partnerID=8YFLogxK
U2 - 10.1074/jbc.M113.514570
DO - 10.1074/jbc.M113.514570
M3 - Article
C2 - 24022488
AN - SCOPUS:84886886742
VL - 288
SP - 30636
EP - 30644
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 42
ER -