Abstract
Background: 3′-Terminal CCA-containing tRNAs and precursors are resistant to action of RNase BN/RNase Z. Results: Arg274 and the two C residues are required for protection by the CCA sequence. Conclusion: Presence of Arg274 and CC sequence prevents RNA substrate from moving into the RNase catalytic site. Significance: This mechanism explains how mature tRNAs are protected from removal of the CCA sequence by a processing RNase.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 30636-30644 |
| Number of pages | 9 |
| Journal | Journal of Biological Chemistry |
| Volume | 288 |
| Issue number | 42 |
| DOIs | |
| State | Published - Oct 18 2013 |
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology
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Dutta, T., Malhotra, A., & Deutscher, M. P. (2013). How a CCA sequence protects mature tRNAs and tRNA precursors from action of the processing enzyme RNase BN/RNase Z. Journal of Biological Chemistry, 288(42), 30636-30644. https://doi.org/10.1074/jbc.M113.514570