How a CCA sequence protects mature tRNAs and tRNA precursors from action of the processing enzyme RNase BN/RNase Z

Tanmay Dutta, Arun Malhotra, Murray P Deutscher

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Background: 3′-Terminal CCA-containing tRNAs and precursors are resistant to action of RNase BN/RNase Z. Results: Arg274 and the two C residues are required for protection by the CCA sequence. Conclusion: Presence of Arg274 and CC sequence prevents RNA substrate from moving into the RNase catalytic site. Significance: This mechanism explains how mature tRNAs are protected from removal of the CCA sequence by a processing RNase.

Original languageEnglish
Pages (from-to)30636-30644
Number of pages9
JournalJournal of Biological Chemistry
Volume288
Issue number42
DOIs
StatePublished - Oct 18 2013

Fingerprint

RNA Precursors
Ribonucleases
Transfer RNA
Enzymes
Processing
Catalytic Domain
RNA
Substrates
ribonuclease BN
RNase Z

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

How a CCA sequence protects mature tRNAs and tRNA precursors from action of the processing enzyme RNase BN/RNase Z. / Dutta, Tanmay; Malhotra, Arun; Deutscher, Murray P.

In: Journal of Biological Chemistry, Vol. 288, No. 42, 18.10.2013, p. 30636-30644.

Research output: Contribution to journalArticle

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