How a CCA sequence protects mature tRNAs and tRNA precursors from action of the processing enzyme RNase BN/RNase Z

Tanmay Dutta; Arun Malhotra; Murray P. Deutscher

doi:10.1074/jbc.M113.514570

How a CCA sequence protects mature tRNAs and tRNA precursors from action of the processing enzyme RNase BN/RNase Z

Tanmay Dutta, Arun Malhotra, Murray P. Deutscher

Biochemistry & Molecular Biology

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Background: 3′-Terminal CCA-containing tRNAs and precursors are resistant to action of RNase BN/RNase Z. Results: Arg274 and the two C residues are required for protection by the CCA sequence. Conclusion: Presence of Arg274 and CC sequence prevents RNA substrate from moving into the RNase catalytic site. Significance: This mechanism explains how mature tRNAs are protected from removal of the CCA sequence by a processing RNase.

Original language	English (US)
Pages (from-to)	30636-30644
Number of pages	9
Journal	Journal of Biological Chemistry
Volume	288
Issue number	42
DOIs	https://doi.org/10.1074/jbc.M113.514570
State	Published - Oct 18 2013

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Cell Biology

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abstract = "Background: 3′-Terminal CCA-containing tRNAs and precursors are resistant to action of RNase BN/RNase Z. Results: Arg274 and the two C residues are required for protection by the CCA sequence. Conclusion: Presence of Arg274 and CC sequence prevents RNA substrate from moving into the RNase catalytic site. Significance: This mechanism explains how mature tRNAs are protected from removal of the CCA sequence by a processing RNase.",

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AU - Malhotra, Arun

AU - Deutscher, Murray P.

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