Host-derived enterocytes in intestinal grafts

Panagiotis Tryphonopoulos, Michael Icardi, Shashikumar Salgar, Phillip Ruiz, Tatsuya Fukumori, Carlos Gandia, Konstantinos Boukas, Tomoaki Kato, Violet Esquenazi, Camillo Ricordi, George Michalopoulos, Joshua Miller, Andreas Tzakis

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


Replacement of donor lymphoid tissue by lymphocytes of recipient origin is an established phenomenon in small bowel transplants. However, replacement of donor epithelial cells of bowel grafts by host cells has not been demonstrated. The objective of our study was to determine whether donor enterocytes are replaced by host-derived enterocytes in the intestinal allograft. Graft biopsy specimens, obtained from five human male recipients of female intestine, were examined for the presence of male enterocytes. The biopsies dated from 90 to 770 days posttransplant. Formalin-fixed 3-μm specimen sections were stained for X and Y chromosomes by fluorescent in situ hybridization technique. Fluorescent microscopy of the stained sections identified male enterocytes in four patients, with a percentage of male cells ranging from 0.09% to 0.26% of the total enterocyte mass. Using the fluorescent in situ hybridization technique, we demonstrated the presence of host-derived male (XY) enterocytes in the female intestinal graft.

Original languageEnglish (US)
Pages (from-to)120-122
Number of pages3
Issue number1
StatePublished - Jul 15 2002

ASJC Scopus subject areas

  • Transplantation


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