Hormone dependence of breast cancer cells and the effects of tamoxifen and estrogen

31P NMR studies

Jesús Ruiz-Cabello, Kirsten Berghmans, Ofer Kaplan, Marc E Lippman, Robert Clarke, Jack S. Cohen

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Many breast tumors appear to progress from estrogen-dependent growth to a more malignant phenotype characterized by estrogen-independent growth, antiestrogen resistance, and a high metastatic potential. Utilizing31P NMR spectroscopy on human breast cancer cells growing in vitro, we have investigated the effects of 17β-estradiol and tamoxifen on the metabolic/bioenergetic spectra of a series of human breast cancer cells that vary in their estrogen and antiestrogen responsiveness. A comparison of baseline spectra associates higher levels of phosphodiesters and UDP-glucosides (e.g. UDP-glucose, UDP-N-acetylglucosamine), and lower phosphocholine/glycerylphosphocholine and phosphocholine/phosphoethanolamine ratios, with the acquisition of estrogen-independent growth in estrogen receptor expressing cells. No metabolic changes are clearly associated with the metastatic phenotype. Whilst estrogen treatment produces no consistently significant spectral changes in any of the cell lines, the estrogen-independent and estrogen-responsive MCF7/MIII cell line responds to tamoxifen treatment by significantly increasing all spectral resonances 30%-40% above baseline values. This may reflect a tamoxifen-induced change to a more differentiated or apoptotic phenotype, or an attempt by the cells to reverse the inhibitory effects of the drug. The ability to detect metabolic changes in response to tamoxifen by NMR spectroscopy may provide a novel means to identify those tumors that are responsive to antiestrogen therapy.

Original languageEnglish
Pages (from-to)209-217
Number of pages9
JournalBreast Cancer Research and Treatment
Volume33
Issue number3
DOIs
StatePublished - Mar 1 1995
Externally publishedYes

Fingerprint

Tamoxifen
Estrogens
Hormones
Breast Neoplasms
Estrogen Receptor Modulators
Phosphorylcholine
Phenotype
Magnetic Resonance Spectroscopy
Growth
Uridine Diphosphate N-Acetylglucosamine
Uridine Diphosphate Glucose
Cell Line
Uridine Diphosphate
MCF-7 Cells
Glucosides
Estrogen Receptors
Energy Metabolism
Estradiol
Pharmaceutical Preparations
Neoplasms

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Ruiz-Cabello, J., Berghmans, K., Kaplan, O., Lippman, M. E., Clarke, R., & Cohen, J. S. (1995). Hormone dependence of breast cancer cells and the effects of tamoxifen and estrogen: 31P NMR studies. Breast Cancer Research and Treatment, 33(3), 209-217. https://doi.org/10.1007/BF00665945

Hormone dependence of breast cancer cells and the effects of tamoxifen and estrogen : 31P NMR studies. / Ruiz-Cabello, Jesús; Berghmans, Kirsten; Kaplan, Ofer; Lippman, Marc E; Clarke, Robert; Cohen, Jack S.

In: Breast Cancer Research and Treatment, Vol. 33, No. 3, 01.03.1995, p. 209-217.

Research output: Contribution to journalArticle

Ruiz-Cabello, J, Berghmans, K, Kaplan, O, Lippman, ME, Clarke, R & Cohen, JS 1995, 'Hormone dependence of breast cancer cells and the effects of tamoxifen and estrogen: 31P NMR studies', Breast Cancer Research and Treatment, vol. 33, no. 3, pp. 209-217. https://doi.org/10.1007/BF00665945
Ruiz-Cabello, Jesús ; Berghmans, Kirsten ; Kaplan, Ofer ; Lippman, Marc E ; Clarke, Robert ; Cohen, Jack S. / Hormone dependence of breast cancer cells and the effects of tamoxifen and estrogen : 31P NMR studies. In: Breast Cancer Research and Treatment. 1995 ; Vol. 33, No. 3. pp. 209-217.
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