Hormonal Regulation of net DNA Synthesis in MCF-7 Human Breast Cancer Cells in Tissue Culture

Susan C. Aitken, Marc E. Lippman

Research output: Contribution to journalArticle

45 Scopus citations

Abstract

Estrogen and anti-estrogens have striking effects on growth of target tissues in vivo. In order to examine these effects in vitro, experimental methods for the rigorous assessment of rates of net DNA synthesis in tissue culture system are required. The quantitative of DNA synthesis with inorganic [32P]orthophosphate is described. The limitations of more conventional [3H]thymidine labeling are illustrated in this hormonally responsive system. Estrogen administration increases the specific activity of acid-soluble phosphate and increases net DNA synthesis relative to controls in MCF-7 human breast cancer cells. Stimulation is most evident after 36 hr of hormone treatment. Conversely, tamoxifen produces substantial inhibition of net DNA synthesis after 36 hr of hormone treatment. Thymidine availability modulates the effect of estrogens and anti-estrogens on DNA synthesis and constitutes an independent experimental variable.

Original languageEnglish (US)
Pages (from-to)1727-1735
Number of pages9
JournalCancer Research
Volume42
Issue number5
StatePublished - May 1 1982

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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