Abstract
By cellular activation with hormones, we test the proposition (Loewenstein, W.R., Physiol. Rev.61:829, 1981) that the permeability of cell junction is upregulated through elevation of the level of cyclic AMP. Cultured rat glioma C-6 cells, with β-adrenergic receptors, and human lung WI-38 cells, with prostaglandin receptors, were exposed to catecholamine (isoproterenol) and prostaglandin E1, respectively, while their junctions were probed with microinjected fluorescent-labelled mono-, di-, and triglutamate. Junctional permeability, as indexed by the proportion of cell interfaces transferring the probes, rose after the hormone treatments. The increase in permeability took several hours to develop and was associated with an increase in the number of gap-junctional membrane particles (freeze-fracture electron microscopy). Such interaction between hormonal and junctional intercellular communication may provide a mechanism for physiological regulation of junctional communication and (perhaps as part of that) for physiological coordination of responses of cells in organs and tissues to hormones.
| Original language | English |
|---|---|
| Pages (from-to) | 239-251 |
| Number of pages | 13 |
| Journal | The Journal of Membrane Biology |
| Volume | 70 |
| Issue number | 3 |
| DOIs | |
| State | Published - Oct 1 1982 |
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Keywords
- catecholamine
- cell junction permeability
- cell-to-cell communication
- cell-to-cell membrane channels
- cyclic AMP
- gap junction
- hormones
- membrane permeability
- prostaglandin E
ASJC Scopus subject areas
- Physiology
- Cell Biology
- Biophysics
Cite this
Hormonal regulation of cell junction permeability : Upregulation by catecholamine and prostaglandin E1. / Radu, A.; Dahl, Gerhard; Loewenstein, W. R.
In: The Journal of Membrane Biology, Vol. 70, No. 3, 01.10.1982, p. 239-251.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Hormonal regulation of cell junction permeability
T2 - Upregulation by catecholamine and prostaglandin E1
AU - Radu, A.
AU - Dahl, Gerhard
AU - Loewenstein, W. R.
PY - 1982/10/1
Y1 - 1982/10/1
N2 - By cellular activation with hormones, we test the proposition (Loewenstein, W.R., Physiol. Rev.61:829, 1981) that the permeability of cell junction is upregulated through elevation of the level of cyclic AMP. Cultured rat glioma C-6 cells, with β-adrenergic receptors, and human lung WI-38 cells, with prostaglandin receptors, were exposed to catecholamine (isoproterenol) and prostaglandin E1, respectively, while their junctions were probed with microinjected fluorescent-labelled mono-, di-, and triglutamate. Junctional permeability, as indexed by the proportion of cell interfaces transferring the probes, rose after the hormone treatments. The increase in permeability took several hours to develop and was associated with an increase in the number of gap-junctional membrane particles (freeze-fracture electron microscopy). Such interaction between hormonal and junctional intercellular communication may provide a mechanism for physiological regulation of junctional communication and (perhaps as part of that) for physiological coordination of responses of cells in organs and tissues to hormones.
AB - By cellular activation with hormones, we test the proposition (Loewenstein, W.R., Physiol. Rev.61:829, 1981) that the permeability of cell junction is upregulated through elevation of the level of cyclic AMP. Cultured rat glioma C-6 cells, with β-adrenergic receptors, and human lung WI-38 cells, with prostaglandin receptors, were exposed to catecholamine (isoproterenol) and prostaglandin E1, respectively, while their junctions were probed with microinjected fluorescent-labelled mono-, di-, and triglutamate. Junctional permeability, as indexed by the proportion of cell interfaces transferring the probes, rose after the hormone treatments. The increase in permeability took several hours to develop and was associated with an increase in the number of gap-junctional membrane particles (freeze-fracture electron microscopy). Such interaction between hormonal and junctional intercellular communication may provide a mechanism for physiological regulation of junctional communication and (perhaps as part of that) for physiological coordination of responses of cells in organs and tissues to hormones.
KW - catecholamine
KW - cell junction permeability
KW - cell-to-cell communication
KW - cell-to-cell membrane channels
KW - cyclic AMP
KW - gap junction
KW - hormones
KW - membrane permeability
KW - prostaglandin E
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UR - http://www.scopus.com/inward/citedby.url?scp=0020307256&partnerID=8YFLogxK
U2 - 10.1007/BF01870566
DO - 10.1007/BF01870566
M3 - Article
C2 - 6313926
AN - SCOPUS:0020307256
VL - 70
SP - 239
EP - 251
JO - Journal of Membrane Biology
JF - Journal of Membrane Biology
SN - 0022-2631
IS - 3
ER -