HOPX functions as a tumour suppressor in head and neck cancer

Lee Fah Yap, Sook Ling Lai, Sathya Narayanan Patmanathan, Ravindran Caspa Gokulan, C. Max Robinson, Joe B. White, San Jiun Chai, Pathmanathan Rajadurai, Narayanan Prepageran, Yew Toong Liew, Victor Lopes, Wenbin Wei, Robert J. Hollows, Paul G. Murray, Daniel W. Lambert, Keith D. Hunter, Ian C. Paterson

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Head and neck squamous cell carcinoma (HNSCC) is generalized term that encompasses a diverse group of cancers that includes tumours of the oral cavity (OSCC), oropharynx (OPSCC) and nasopharynx (NPC). Genetic alterations that are common to all HNSCC types are likely to be important for squamous carcinogenesis. In this study, we have investigated the role of the homeodomain-only homeobox gene, HOPX, in the pathogenesis of HNSCC. We show that HOPX mRNA levels are reduced in OSCC and NPC cell lines and tissues and there is a general reduction of HOPX protein expression in these tumours and OPSCCs. HOPX promoter methylation was observed in a subset of HNSCCs and was associated with a worse overall survival in HPV negative tumours. RNAseq analysis of OSCC cells transfected with HOPX revealed a widespread deregulation of the transcription of genes related to epithelial homeostasis and ectopic over-expression of HOPX in OSCC and NPC cells inhibited cell proliferation, plating efficiency and migration, and enhanced sensitivity to UVA-induced apoptosis. Our results demonstrate that HOPX functions as a tumour suppressor in HNSCC and suggest a central role for HOPX in suppressing epithelial carcinogenesis.

Original languageEnglish (US)
Article number38758
JournalScientific Reports
Volume6
DOIs
StatePublished - Dec 9 2016
Externally publishedYes

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Head and Neck Neoplasms
Neoplasms
Carcinogenesis
Oropharynx
Nasopharynx
Homeobox Genes
Methylation
Mouth
Homeostasis
Cell Proliferation
Apoptosis
Cell Line
Messenger RNA
Carcinoma, squamous cell of head and neck
Genes
Proteins

ASJC Scopus subject areas

  • General

Cite this

Yap, L. F., Lai, S. L., Patmanathan, S. N., Caspa Gokulan, R., Robinson, C. M., White, J. B., ... Paterson, I. C. (2016). HOPX functions as a tumour suppressor in head and neck cancer. Scientific Reports, 6, [38758]. https://doi.org/10.1038/srep38758

HOPX functions as a tumour suppressor in head and neck cancer. / Yap, Lee Fah; Lai, Sook Ling; Patmanathan, Sathya Narayanan; Caspa Gokulan, Ravindran; Robinson, C. Max; White, Joe B.; Chai, San Jiun; Rajadurai, Pathmanathan; Prepageran, Narayanan; Liew, Yew Toong; Lopes, Victor; Wei, Wenbin; Hollows, Robert J.; Murray, Paul G.; Lambert, Daniel W.; Hunter, Keith D.; Paterson, Ian C.

In: Scientific Reports, Vol. 6, 38758, 09.12.2016.

Research output: Contribution to journalArticle

Yap, LF, Lai, SL, Patmanathan, SN, Caspa Gokulan, R, Robinson, CM, White, JB, Chai, SJ, Rajadurai, P, Prepageran, N, Liew, YT, Lopes, V, Wei, W, Hollows, RJ, Murray, PG, Lambert, DW, Hunter, KD & Paterson, IC 2016, 'HOPX functions as a tumour suppressor in head and neck cancer', Scientific Reports, vol. 6, 38758. https://doi.org/10.1038/srep38758
Yap LF, Lai SL, Patmanathan SN, Caspa Gokulan R, Robinson CM, White JB et al. HOPX functions as a tumour suppressor in head and neck cancer. Scientific Reports. 2016 Dec 9;6. 38758. https://doi.org/10.1038/srep38758
Yap, Lee Fah ; Lai, Sook Ling ; Patmanathan, Sathya Narayanan ; Caspa Gokulan, Ravindran ; Robinson, C. Max ; White, Joe B. ; Chai, San Jiun ; Rajadurai, Pathmanathan ; Prepageran, Narayanan ; Liew, Yew Toong ; Lopes, Victor ; Wei, Wenbin ; Hollows, Robert J. ; Murray, Paul G. ; Lambert, Daniel W. ; Hunter, Keith D. ; Paterson, Ian C. / HOPX functions as a tumour suppressor in head and neck cancer. In: Scientific Reports. 2016 ; Vol. 6.
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