Homologous and heterologous induction of the human bradykinin B1-receptor and B1-receptor localisation along the rat nephron

Joost P. Schanstra, Maria E. Marin-Castaño, Celine Alric, João B. Pesquero, Michel Claire, Jean Pierre Girolami, Jean Loup Bascands

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The kinin B1-receptor which is absent or expressed at very low levels under physiological conditions is strongly induced under inflammatory conditions. It has been shown that B1-receptor induction during inflammation involves interleukin-1β (IL-1β) production and activation of nuclear factor-κB (NF-κB). Since bradykinin (BK), the B2-receptor agonist induces IL-1β expression and activates NF-κB, we have analysed the effect of B2-receptor activation in cultured human lung fibroblasts cells on B1-receptor expression by a semiquantitative RT-PCR analysis. Treatment with BK resulted in a significant increase in the expression of B1-receptor mRNA which was abolished by a specific B2-receptor antagonist. This result suggests that B2-receptor activation can prime the expression of B1-receptors. Although the renal localisation of the B2-receptor has been thoroughly studied, nothing is known about the distribution of the B1-receptor in the kidney. Using a combination of microdissection and a semiquantitative RT-PCR/Southern blot analysis we showed the absence of B1-receptors under physiological conditions in 10 microdissected rat nephron segments. However, 18 h LPS-treatment induced significant expression of the B1-receptor in all, but one segment. These studies provide the first molecular basis for the observed changes in renal haemodynamics after B1-agonist infusion in animal kidney models. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)29-34
Number of pages6
JournalImmunopharmacology
Volume45
Issue number1-3
DOIs
StatePublished - Dec 1 1999
Externally publishedYes

Fingerprint

Bradykinin B1 Receptors
Nephrons
Kidney
Interleukin-1
Bradykinin B2 Receptors
Polymerase Chain Reaction
Microdissection
Kinins
Bradykinin
Southern Blotting
Animal Models
Fibroblasts
Hemodynamics
Inflammation
Lung
Messenger RNA

Keywords

  • Bradykinin B1-receptor
  • Inflammation
  • Microdissection
  • Nephron
  • Up-regulation

ASJC Scopus subject areas

  • Pharmacology

Cite this

Schanstra, J. P., Marin-Castaño, M. E., Alric, C., Pesquero, J. B., Claire, M., Girolami, J. P., & Bascands, J. L. (1999). Homologous and heterologous induction of the human bradykinin B1-receptor and B1-receptor localisation along the rat nephron. Immunopharmacology, 45(1-3), 29-34. https://doi.org/10.1016/S0162-3109(99)00054-5

Homologous and heterologous induction of the human bradykinin B1-receptor and B1-receptor localisation along the rat nephron. / Schanstra, Joost P.; Marin-Castaño, Maria E.; Alric, Celine; Pesquero, João B.; Claire, Michel; Girolami, Jean Pierre; Bascands, Jean Loup.

In: Immunopharmacology, Vol. 45, No. 1-3, 01.12.1999, p. 29-34.

Research output: Contribution to journalArticle

Schanstra, JP, Marin-Castaño, ME, Alric, C, Pesquero, JB, Claire, M, Girolami, JP & Bascands, JL 1999, 'Homologous and heterologous induction of the human bradykinin B1-receptor and B1-receptor localisation along the rat nephron', Immunopharmacology, vol. 45, no. 1-3, pp. 29-34. https://doi.org/10.1016/S0162-3109(99)00054-5
Schanstra, Joost P. ; Marin-Castaño, Maria E. ; Alric, Celine ; Pesquero, João B. ; Claire, Michel ; Girolami, Jean Pierre ; Bascands, Jean Loup. / Homologous and heterologous induction of the human bradykinin B1-receptor and B1-receptor localisation along the rat nephron. In: Immunopharmacology. 1999 ; Vol. 45, No. 1-3. pp. 29-34.
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