Homologous and heterologous induction of the human bradykinin B1-receptor and B1-receptor localisation along the rat nephron

Joost P. Schanstra, Maria E. Marin-Castaño, Celine Alric, João B. Pesquero, Michel Claire, Jean Pierre Girolami, Jean Loup Bascands

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5 Scopus citations

Abstract

The kinin B1-receptor which is absent or expressed at very low levels under physiological conditions is strongly induced under inflammatory conditions. It has been shown that B1-receptor induction during inflammation involves interleukin-1β (IL-1β) production and activation of nuclear factor-κB (NF-κB). Since bradykinin (BK), the B2-receptor agonist induces IL-1β expression and activates NF-κB, we have analysed the effect of B2-receptor activation in cultured human lung fibroblasts cells on B1-receptor expression by a semiquantitative RT-PCR analysis. Treatment with BK resulted in a significant increase in the expression of B1-receptor mRNA which was abolished by a specific B2-receptor antagonist. This result suggests that B2-receptor activation can prime the expression of B1-receptors. Although the renal localisation of the B2-receptor has been thoroughly studied, nothing is known about the distribution of the B1-receptor in the kidney. Using a combination of microdissection and a semiquantitative RT-PCR/Southern blot analysis we showed the absence of B1-receptors under physiological conditions in 10 microdissected rat nephron segments. However, 18 h LPS-treatment induced significant expression of the B1-receptor in all, but one segment. These studies provide the first molecular basis for the observed changes in renal haemodynamics after B1-agonist infusion in animal kidney models. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)29-34
Number of pages6
JournalImmunopharmacology
Volume45
Issue number1-3
DOIs
StatePublished - Dec 1 1999

Keywords

  • Bradykinin B1-receptor
  • Inflammation
  • Microdissection
  • Nephron
  • Up-regulation

ASJC Scopus subject areas

  • Pharmacology

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    Schanstra, J. P., Marin-Castaño, M. E., Alric, C., Pesquero, J. B., Claire, M., Girolami, J. P., & Bascands, J. L. (1999). Homologous and heterologous induction of the human bradykinin B1-receptor and B1-receptor localisation along the rat nephron. Immunopharmacology, 45(1-3), 29-34. https://doi.org/10.1016/S0162-3109(99)00054-5