Homodimeric alkaline phosphatase located at Helicoverpa armigera Midgut, a putat ive receptor of Cry1Ac contains α-GaINAc in term inal glycan structure as interactive Epitope

Anindya Sarkar, Daniel Hess, Hossain A. Mondal, Santanu Banerjee, Hari C. Sharma, Sampa Das

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Helicoverpa armigera causes massive yield loss of various crops globally. Bacillus thuringiensis coded Cry1Ac toxin is effective against the pest. Through the present investigation, an alkaline phosphatase type putative receptor of the toxin was identified in the target insect gut BBMV. Lectin-ligand immunoblot assay detected the presence of α-GalNAc residue at the nonreducing terminal of the glycan structure in the membrane bound HaALP protein which mediates the toxin-receptor interaction.

Original languageEnglish (US)
Pages (from-to)1838-1848
Number of pages11
JournalJournal of Proteome Research
Volume8
Issue number4
DOIs
StatePublished - Apr 3 2009
Externally publishedYes

Fingerprint

Bacillus thuringiensis
Bacilli
Lectins
Crops
Polysaccharides
Alkaline Phosphatase
Insects
Epitopes
Assays
Ligands
Membranes
Proteins

Keywords

  • Alkaline phosphatase
  • Cry l Ac
  • Helicoverpa armigera
  • Putative receptor
  • Toxin receptor interaction

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Cite this

Homodimeric alkaline phosphatase located at Helicoverpa armigera Midgut, a putat ive receptor of Cry1Ac contains α-GaINAc in term inal glycan structure as interactive Epitope. / Sarkar, Anindya; Hess, Daniel; Mondal, Hossain A.; Banerjee, Santanu; Sharma, Hari C.; Das, Sampa.

In: Journal of Proteome Research, Vol. 8, No. 4, 03.04.2009, p. 1838-1848.

Research output: Contribution to journalArticle

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