HIV infects glomerular endothelial and mesangial but not epithelial cells in vitro

Dollie F. Green; Lionel Resnick; Jacques J. Bourgoignie

doi:10.1038/ki.1992.146

HIV infects glomerular endothelial and mesangial but not epithelial cells in vitro

Dollie F. Green, Lionel Resnick, Jacques J. Bourgoignie

Medicine

Research output: Contribution to journal › Article › peer-review

97 Scopus citations

Abstract

The infectivity of human immunodeficiency virus (HIV-1) in human glomerular cells was evaluated by exposing homogeneous cultures of human glomerular capillary endothelial, mesangial and epithelial cells to HIV in vitro. Infectivity and HIV expression was assessed by: 1) the measurement of p24 antigen production from culture supernatants; 2) the presence of p24 antigen intracellularly by immunofluorescence; 3) levels of P24 antigen production or syncytia formation following the cocultivation of glomerular cells exposed to HIV with normal human peripheral blood mononuclear cells or MT-2 lymphocytes; and 4) the presence of intracellular HIV DNA by polymerase chain reaction. The results indicate that HIV can infect and replicate in glomerular capillary endothelial cells and in a small percentage of mesangial cells, but not in human glomerular epithelial cells in vitro.

Original language	English (US)
Pages (from-to)	956-960
Number of pages	5
Journal	Kidney international
Volume	41
Issue number	4
DOIs	https://doi.org/10.1038/ki.1992.146
State	Published - Apr 1992

ASJC Scopus subject areas

Nephrology

UN SDGs

This output contributes to the following Sustainable Development Goal(s)

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title = "HIV infects glomerular endothelial and mesangial but not epithelial cells in vitro",

abstract = "The infectivity of human immunodeficiency virus (HIV-1) in human glomerular cells was evaluated by exposing homogeneous cultures of human glomerular capillary endothelial, mesangial and epithelial cells to HIV in vitro. Infectivity and HIV expression was assessed by: 1) the measurement of p24 antigen production from culture supernatants; 2) the presence of p24 antigen intracellularly by immunofluorescence; 3) levels of P24 antigen production or syncytia formation following the cocultivation of glomerular cells exposed to HIV with normal human peripheral blood mononuclear cells or MT-2 lymphocytes; and 4) the presence of intracellular HIV DNA by polymerase chain reaction. The results indicate that HIV can infect and replicate in glomerular capillary endothelial cells and in a small percentage of mesangial cells, but not in human glomerular epithelial cells in vitro.",

author = "Green, {Dollie F.} and Lionel Resnick and Bourgoignie, {Jacques J.}",

note = "Funding Information: This work was supported by NIH Grants R01-DK 37901 and DK",

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AU - Green, Dollie F.

AU - Resnick, Lionel

AU - Bourgoignie, Jacques J.

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AB - The infectivity of human immunodeficiency virus (HIV-1) in human glomerular cells was evaluated by exposing homogeneous cultures of human glomerular capillary endothelial, mesangial and epithelial cells to HIV in vitro. Infectivity and HIV expression was assessed by: 1) the measurement of p24 antigen production from culture supernatants; 2) the presence of p24 antigen intracellularly by immunofluorescence; 3) levels of P24 antigen production or syncytia formation following the cocultivation of glomerular cells exposed to HIV with normal human peripheral blood mononuclear cells or MT-2 lymphocytes; and 4) the presence of intracellular HIV DNA by polymerase chain reaction. The results indicate that HIV can infect and replicate in glomerular capillary endothelial cells and in a small percentage of mesangial cells, but not in human glomerular epithelial cells in vitro.

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HIV infects glomerular endothelial and mesangial but not epithelial cells in vitro

Abstract

ASJC Scopus subject areas

UN SDGs

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