HIV infects glomerular endothelial and mesangial but not epithelial cells in vitro

Dollie F Green, Lionel Resnick, Jacques J. Bourgoignie

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

The infectivity of human immunodeficiency virus (HIV-1) in human glomerular cells was evaluated by exposing homogeneous cultures of human glomerular capillary endothelial, mesangial and epithelial cells to HIV in vitro. Infectivity and HIV expression was assessed by: 1) the measurement of p24 antigen production from culture supernatants; 2) the presence of p24 antigen intracellularly by immunofluorescence; 3) levels of P24 antigen production or syncytia formation following the cocultivation of glomerular cells exposed to HIV with normal human peripheral blood mononuclear cells or MT-2 lymphocytes; and 4) the presence of intracellular HIV DNA by polymerase chain reaction. The results indicate that HIV can infect and replicate in glomerular capillary endothelial cells and in a small percentage of mesangial cells, but not in human glomerular epithelial cells in vitro.

Original languageEnglish
Pages (from-to)956-960
Number of pages5
JournalKidney International
Volume41
Issue number4
StatePublished - Apr 1 1992

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Epithelial Cells
HIV
Mesangial Cells
Antigens
HIV-1
Endothelial Cells
Giant Cells
DNA-Directed DNA Polymerase
Coculture Techniques
Fluorescent Antibody Technique
Blood Cells
In Vitro Techniques
Lymphocytes
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Nephrology

Cite this

Green, D. F., Resnick, L., & Bourgoignie, J. J. (1992). HIV infects glomerular endothelial and mesangial but not epithelial cells in vitro. Kidney International, 41(4), 956-960.

HIV infects glomerular endothelial and mesangial but not epithelial cells in vitro. / Green, Dollie F; Resnick, Lionel; Bourgoignie, Jacques J.

In: Kidney International, Vol. 41, No. 4, 01.04.1992, p. 956-960.

Research output: Contribution to journalArticle

Green, DF, Resnick, L & Bourgoignie, JJ 1992, 'HIV infects glomerular endothelial and mesangial but not epithelial cells in vitro', Kidney International, vol. 41, no. 4, pp. 956-960.
Green, Dollie F ; Resnick, Lionel ; Bourgoignie, Jacques J. / HIV infects glomerular endothelial and mesangial but not epithelial cells in vitro. In: Kidney International. 1992 ; Vol. 41, No. 4. pp. 956-960.
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