Abstract
The infectivity of human immunodeficiency virus (HIV-1) in human glomerular cells was evaluated by exposing homogeneous cultures of human glomerular capillary endothelial, mesangial and epithelial cells to HIV in vitro. Infectivity and HIV expression was assessed by: 1) the measurement of p24 antigen production from culture supernatants; 2) the presence of p24 antigen intracellularly by immunofluorescence; 3) levels of P24 antigen production or syncytia formation following the cocultivation of glomerular cells exposed to HIV with normal human peripheral blood mononuclear cells or MT-2 lymphocytes; and 4) the presence of intracellular HIV DNA by polymerase chain reaction. The results indicate that HIV can infect and replicate in glomerular capillary endothelial cells and in a small percentage of mesangial cells, but not in human glomerular epithelial cells in vitro.
| Original language | English |
|---|---|
| Pages (from-to) | 956-960 |
| Number of pages | 5 |
| Journal | Kidney International |
| Volume | 41 |
| Issue number | 4 |
| State | Published - Apr 1 1992 |
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ASJC Scopus subject areas
- Nephrology
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HIV infects glomerular endothelial and mesangial but not epithelial cells in vitro. / Green, Dollie F; Resnick, Lionel; Bourgoignie, Jacques J.
In: Kidney International, Vol. 41, No. 4, 01.04.1992, p. 956-960.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - HIV infects glomerular endothelial and mesangial but not epithelial cells in vitro
AU - Green, Dollie F
AU - Resnick, Lionel
AU - Bourgoignie, Jacques J.
PY - 1992/4/1
Y1 - 1992/4/1
N2 - The infectivity of human immunodeficiency virus (HIV-1) in human glomerular cells was evaluated by exposing homogeneous cultures of human glomerular capillary endothelial, mesangial and epithelial cells to HIV in vitro. Infectivity and HIV expression was assessed by: 1) the measurement of p24 antigen production from culture supernatants; 2) the presence of p24 antigen intracellularly by immunofluorescence; 3) levels of P24 antigen production or syncytia formation following the cocultivation of glomerular cells exposed to HIV with normal human peripheral blood mononuclear cells or MT-2 lymphocytes; and 4) the presence of intracellular HIV DNA by polymerase chain reaction. The results indicate that HIV can infect and replicate in glomerular capillary endothelial cells and in a small percentage of mesangial cells, but not in human glomerular epithelial cells in vitro.
AB - The infectivity of human immunodeficiency virus (HIV-1) in human glomerular cells was evaluated by exposing homogeneous cultures of human glomerular capillary endothelial, mesangial and epithelial cells to HIV in vitro. Infectivity and HIV expression was assessed by: 1) the measurement of p24 antigen production from culture supernatants; 2) the presence of p24 antigen intracellularly by immunofluorescence; 3) levels of P24 antigen production or syncytia formation following the cocultivation of glomerular cells exposed to HIV with normal human peripheral blood mononuclear cells or MT-2 lymphocytes; and 4) the presence of intracellular HIV DNA by polymerase chain reaction. The results indicate that HIV can infect and replicate in glomerular capillary endothelial cells and in a small percentage of mesangial cells, but not in human glomerular epithelial cells in vitro.
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M3 - Article
C2 - 1513116
AN - SCOPUS:0026510175
VL - 41
SP - 956
EP - 960
JO - Kidney International
JF - Kidney International
SN - 0085-2538
IS - 4
ER -