HIV-1 RNA load in needles/syringes from shooting galleries in Miami: A preliminary laboratory report

Paul Shapshak, Robert K. Fujimura, J. Bryan Page, David Segal, James E. Rivers, Jun Yang, Syed M. Shah, Garth Graham, Lisa Metsch, Norman Weatherby, Dale D. Chitwood, Clyde B. McCoy

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

We quantified HIV-1 RNA load in rinses from needles/syringes (N/S) obtained at shooting galleries in Miami and also analyzed the rinses for antibodies for viral proteins. In rinses from 36 N/S that contained visible blood, 14 (39%) had detectable amounts of HIV-1 RNA. Numbers of copies of HIV-1 RNA ranged from the detection limit (400 copies/ml) to 268,000 copies/ml. We also detected antibodies to HIV-1 polypeptides in 34/36 (94%) of rinses from visibly contaminated N/S using Western blots specific for the HIV-1 proteins. No antibodies were detected in laboratory rinses from six visibly clean needles. The presence of HIV-1 RNA in N/S is an important indication of the risk created by N/S sharing as well as by shared paraphernalia and wash waters by injecting drug users. Copyright (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish (US)
Pages (from-to)153-157
Number of pages5
JournalDrug and Alcohol Dependence
Volume58
Issue number1-2
DOIs
StatePublished - Feb 1 2000

Keywords

  • AIDS
  • Blood
  • HIV-1 RNA load
  • Injecting drug user
  • Needle/syringe
  • Paraphernalia
  • Shooting gallery

ASJC Scopus subject areas

  • Medicine(all)
  • Behavioral Neuroscience
  • Toxicology
  • Health(social science)

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  • Cite this

    Shapshak, P., Fujimura, R. K., Page, J. B., Segal, D., Rivers, J. E., Yang, J., Shah, S. M., Graham, G., Metsch, L., Weatherby, N., Chitwood, D. D., & McCoy, C. B. (2000). HIV-1 RNA load in needles/syringes from shooting galleries in Miami: A preliminary laboratory report. Drug and Alcohol Dependence, 58(1-2), 153-157. https://doi.org/10.1016/S0376-8716(99)00085-X