HIV-1 RNA load in needles/syringes from shooting galleries in Miami: A preliminary laboratory report

Paul Shapshak, Robert K. Fujimura, John Page, David Segal, James E. Rivers, Jun Yang, Syed M. Shah, Garth Graham, Lisa Metsch, Norman Weatherby, Dale D. Chitwood, Clyde B McCoy

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

We quantified HIV-1 RNA load in rinses from needles/syringes (N/S) obtained at shooting galleries in Miami and also analyzed the rinses for antibodies for viral proteins. In rinses from 36 N/S that contained visible blood, 14 (39%) had detectable amounts of HIV-1 RNA. Numbers of copies of HIV-1 RNA ranged from the detection limit (400 copies/ml) to 268,000 copies/ml. We also detected antibodies to HIV-1 polypeptides in 34/36 (94%) of rinses from visibly contaminated N/S using Western blots specific for the HIV-1 proteins. No antibodies were detected in laboratory rinses from six visibly clean needles. The presence of HIV-1 RNA in N/S is an important indication of the risk created by N/S sharing as well as by shared paraphernalia and wash waters by injecting drug users. Copyright (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)153-157
Number of pages5
JournalDrug and Alcohol Dependence
Volume58
Issue number1-2
DOIs
StatePublished - Feb 1 2000

Fingerprint

Syringes
Needles
HIV-1
indication
RNA
drug
water
Needle Sharing
Antibodies
Human Immunodeficiency Virus Proteins
Viral Proteins
Drug Users
Limit of Detection
Blood
Western Blotting
Peptides
Water
Pharmaceutical Preparations
Proteins

Keywords

  • AIDS
  • Blood
  • HIV-1 RNA load
  • Injecting drug user
  • Needle/syringe
  • Paraphernalia
  • Shooting gallery

ASJC Scopus subject areas

  • Medicine(all)
  • Behavioral Neuroscience
  • Toxicology
  • Health(social science)

Cite this

HIV-1 RNA load in needles/syringes from shooting galleries in Miami : A preliminary laboratory report. / Shapshak, Paul; Fujimura, Robert K.; Page, John; Segal, David; Rivers, James E.; Yang, Jun; Shah, Syed M.; Graham, Garth; Metsch, Lisa; Weatherby, Norman; Chitwood, Dale D.; McCoy, Clyde B.

In: Drug and Alcohol Dependence, Vol. 58, No. 1-2, 01.02.2000, p. 153-157.

Research output: Contribution to journalArticle

Shapshak, P, Fujimura, RK, Page, J, Segal, D, Rivers, JE, Yang, J, Shah, SM, Graham, G, Metsch, L, Weatherby, N, Chitwood, DD & McCoy, CB 2000, 'HIV-1 RNA load in needles/syringes from shooting galleries in Miami: A preliminary laboratory report', Drug and Alcohol Dependence, vol. 58, no. 1-2, pp. 153-157. https://doi.org/10.1016/S0376-8716(99)00085-X
Shapshak, Paul ; Fujimura, Robert K. ; Page, John ; Segal, David ; Rivers, James E. ; Yang, Jun ; Shah, Syed M. ; Graham, Garth ; Metsch, Lisa ; Weatherby, Norman ; Chitwood, Dale D. ; McCoy, Clyde B. / HIV-1 RNA load in needles/syringes from shooting galleries in Miami : A preliminary laboratory report. In: Drug and Alcohol Dependence. 2000 ; Vol. 58, No. 1-2. pp. 153-157.
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