HIV-1 proviral DNA load across neuroanatomic regions of individuals with evidence for HIV-1-associated dementia

Robert K. Fujimura, Karl Goodkin, Carol Petito, Richard Douyon, Daniel J Feaster, Mauricio Concha, Paul Shapshak

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

A definitive relation between HIV-1 load and the clinical diagnosis of HIV-1-associated dementia (HAD) has not yet been established. Knowledge of the neuroanatomic distribution of HIV-1 load in the brain of individuals with HAD and HIV-1 encephalitis may facilitate elucidation of this relation. Nine individuals with AIDS were analyzed postmortem by three independent methods with each assessment performed blinded to the others: 1) a neuropsychiatric review of clinical records for evidence of possible HAD, 2) HIV-1 DNA load determination by quantitative polymerase chain reaction (PCR) across several neuroanatomic regions, and 3) a pathologic examination for diagnosis of HIV- 1 encephalitis by immunohistochemical techniques. Of eight AIDS cases with clinical records sufficient for neuropsychiatric review, seven were shown to have evidence for HAD. HIV-1 DNA was detected and quantified in specimens from all of the medial temporal lobe regions analyzed but was not detectable in the frontal lobe at the same level of sensitivity in two of these cases (<1 per 1000 cellular genomes). HIV-1 DNA load in the medial temporal lobe region was significantly larger than that in the frontal lobe. Only four of seven cases with evidence for HAD were also diagnosed with HIV-1 encephalitis.

Original languageEnglish
Pages (from-to)146-152
Number of pages7
JournalJournal of Acquired Immune Deficiency Syndromes and Human Retrovirology
Volume16
Issue number3
StatePublished - Nov 1 1997

Fingerprint

Dementia
HIV-1
DNA
Temporal Lobe
Encephalitis
Frontal Lobe
Acquired Immunodeficiency Syndrome
Genome
Polymerase Chain Reaction

Keywords

  • HIV-1
  • HIV-1 encephalitis
  • HIV-1- associated dementia
  • Neuroanatomic regions
  • Proviral DNA load
  • Quantitative PCR

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Virology

Cite this

HIV-1 proviral DNA load across neuroanatomic regions of individuals with evidence for HIV-1-associated dementia. / Fujimura, Robert K.; Goodkin, Karl; Petito, Carol; Douyon, Richard; Feaster, Daniel J; Concha, Mauricio; Shapshak, Paul.

In: Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology, Vol. 16, No. 3, 01.11.1997, p. 146-152.

Research output: Contribution to journalArticle

Fujimura, Robert K. ; Goodkin, Karl ; Petito, Carol ; Douyon, Richard ; Feaster, Daniel J ; Concha, Mauricio ; Shapshak, Paul. / HIV-1 proviral DNA load across neuroanatomic regions of individuals with evidence for HIV-1-associated dementia. In: Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology. 1997 ; Vol. 16, No. 3. pp. 146-152.
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