HIV-1 nef mediates lymphocyte chemotaxis and activation by infected macrophages

S. Swingler, A. Mann, J. M. Jacqué, B. Brichacek, V. G. Sasseville, K. Williams, A. A. Lackner, E. N. Janoff, R. Wang, D. Fisher, Mario Stevenson

Research output: Contribution to journalArticle

299 Citations (Scopus)

Abstract

Infection of macrophage lineage cells is a feature of primate lentivirus replication, and several properties of primate lentiviruses seem to have evolved to promote the infection of macrophages. Here we demonstrate that the accessory gene product Nef induces the production of two CC-chemokines, macrophage inflammatory proteins 1α and 1β, by HIV-1-infected macrophages. Adenovirus-mediated expression of Nef in primary macrophages was sufficient for chemokine induction. Supernatants from Nef-expressing macrophages induced both the chemotaxis and activation of resting T lymphocytes, permitting productive HIV-1 infection. These results indicate a role for Nef in lymphocyte recruitment and activation at sites of virus replication.

Original languageEnglish
Pages (from-to)997-1003
Number of pages7
JournalNature Medicine
Volume5
Issue number9
DOIs
StatePublished - Sep 1 1999
Externally publishedYes

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Lymphocytes
Macrophages
Chemotaxis
Lymphocyte Activation
HIV-1
Chemical activation
Primate Lentiviruses
nef Gene Products
Macrophage Inflammatory Proteins
CC Chemokines
T-cells
Accessories
Virus Replication
Infection
Viruses
Chemokines
Adenoviridae
HIV Infections
T-Lymphocytes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Swingler, S., Mann, A., Jacqué, J. M., Brichacek, B., Sasseville, V. G., Williams, K., ... Stevenson, M. (1999). HIV-1 nef mediates lymphocyte chemotaxis and activation by infected macrophages. Nature Medicine, 5(9), 997-1003. https://doi.org/10.1038/12433

HIV-1 nef mediates lymphocyte chemotaxis and activation by infected macrophages. / Swingler, S.; Mann, A.; Jacqué, J. M.; Brichacek, B.; Sasseville, V. G.; Williams, K.; Lackner, A. A.; Janoff, E. N.; Wang, R.; Fisher, D.; Stevenson, Mario.

In: Nature Medicine, Vol. 5, No. 9, 01.09.1999, p. 997-1003.

Research output: Contribution to journalArticle

Swingler, S, Mann, A, Jacqué, JM, Brichacek, B, Sasseville, VG, Williams, K, Lackner, AA, Janoff, EN, Wang, R, Fisher, D & Stevenson, M 1999, 'HIV-1 nef mediates lymphocyte chemotaxis and activation by infected macrophages', Nature Medicine, vol. 5, no. 9, pp. 997-1003. https://doi.org/10.1038/12433
Swingler S, Mann A, Jacqué JM, Brichacek B, Sasseville VG, Williams K et al. HIV-1 nef mediates lymphocyte chemotaxis and activation by infected macrophages. Nature Medicine. 1999 Sep 1;5(9):997-1003. https://doi.org/10.1038/12433
Swingler, S. ; Mann, A. ; Jacqué, J. M. ; Brichacek, B. ; Sasseville, V. G. ; Williams, K. ; Lackner, A. A. ; Janoff, E. N. ; Wang, R. ; Fisher, D. ; Stevenson, Mario. / HIV-1 nef mediates lymphocyte chemotaxis and activation by infected macrophages. In: Nature Medicine. 1999 ; Vol. 5, No. 9. pp. 997-1003.
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