HIV-1 nef mediates lymphocyte chemotaxis and activation by infected macrophages

S. Swingler, A. Mann, J. M. Jacqué, B. Brichacek, V. G. Sasseville, K. Williams, A. A. Lackner, E. N. Janoff, R. Wang, D. Fisher, M. Stevenson

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303 Scopus citations

Abstract

Infection of macrophage lineage cells is a feature of primate lentivirus replication, and several properties of primate lentiviruses seem to have evolved to promote the infection of macrophages. Here we demonstrate that the accessory gene product Nef induces the production of two CC-chemokines, macrophage inflammatory proteins 1α and 1β, by HIV-1-infected macrophages. Adenovirus-mediated expression of Nef in primary macrophages was sufficient for chemokine induction. Supernatants from Nef-expressing macrophages induced both the chemotaxis and activation of resting T lymphocytes, permitting productive HIV-1 infection. These results indicate a role for Nef in lymphocyte recruitment and activation at sites of virus replication.

Original languageEnglish (US)
Pages (from-to)997-1003
Number of pages7
JournalNature medicine
Volume5
Issue number9
DOIs
StatePublished - Sep 1 1999
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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    Swingler, S., Mann, A., Jacqué, J. M., Brichacek, B., Sasseville, V. G., Williams, K., Lackner, A. A., Janoff, E. N., Wang, R., Fisher, D., & Stevenson, M. (1999). HIV-1 nef mediates lymphocyte chemotaxis and activation by infected macrophages. Nature medicine, 5(9), 997-1003. https://doi.org/10.1038/12433