HIV-1 Infection Is Blocked at an Early Stage in Cells Devoid of Mitochondrial DNA

Gaofei Lu, Suzanne E. Matsuura, Antonio Barrientos, Walter A. Scott

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Human immunodeficiency virus type I (HIV-1) exploits various host cellular pathways for efficient infection. Here we report that the absence of mitochondrial DNA (mtDNA) in ρ0 cells markedly attenuates HIV-1 infection. Importantly, reduced infection efficiency in ρ0 cells is not simply the result of impaired oxidative phosphorylation (OXPHOS) because pharmacological OXPHOS inhibition did not inhibit HIV-1 infection. Analysis of the early steps of virus infection by real-time PCR quantification of stage-specific HIV-1 DNA products in the infected ρ0 and parental cell line have allowed us to conclude that HIV-1 infection in ρ0 cells is blocked at the steps that occur after reverse transcription and prior to nuclear import. Additionally, confocal fluorescence microscope analysis showed that the majority of viral complexes containing HIV-1 p24 co-localize with mitochondria in target cells, suggesting an interaction between the two. Collectively, our data strongly indicate that mitochondria play an important role during early stages of HIV-1 infection, probably through direct association with HIV-1 intracellular complexes.

Original languageEnglish
Article numbere78035
JournalPLoS One
Volume8
Issue number10
DOIs
StatePublished - Oct 21 2013

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Mitochondria
Human immunodeficiency virus 1
Mitochondrial DNA
Viruses
HIV Infections
HIV-1
mitochondrial DNA
Transcription
infection
Microscopes
Fluorescence
Cells
cells
oxidative phosphorylation
DNA
Oxidative Phosphorylation
mitochondria
reverse transcription
Cell Nucleus Active Transport
Human immunodeficiency virus

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

HIV-1 Infection Is Blocked at an Early Stage in Cells Devoid of Mitochondrial DNA. / Lu, Gaofei; Matsuura, Suzanne E.; Barrientos, Antonio; Scott, Walter A.

In: PLoS One, Vol. 8, No. 10, e78035, 21.10.2013.

Research output: Contribution to journalArticle

Lu, Gaofei ; Matsuura, Suzanne E. ; Barrientos, Antonio ; Scott, Walter A. / HIV-1 Infection Is Blocked at an Early Stage in Cells Devoid of Mitochondrial DNA. In: PLoS One. 2013 ; Vol. 8, No. 10.
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