Abstract
Objective: The presence of HIV-1 in postmortem brain tissue from 31 patients with AIDS and 12 HIV-1-negative controls was investigated. Design: Most laboratories have access to the methods used. We readily applied in situ hybridization and immunohistochemistry to archival formalin-fixed paraffin-embedded (FFPE) brain specimens. Methods: The techniques used to detect HIV-1 were explant culture, in situ hybridization with 35S-labeled polymerase (pol) gene riboprobes and immunohistochemistry with monoclonal antibody to gp41. Results: HIV-1 was isolated from explant cultures in 13 out of 20 (65%) patients, whereas HIV-1-infected cells were detected in FFPE brain tissue from nine out of 26 (35%) patients examined by in situ hybridization and in seven out of 26 (27%) patients examined by immunohistochemistry. Conclusions: Although the isolation technique was the most sensitive of the three techniques tested, infected cells may be identified with in situ hybridization in conjunction with immunohistochemistry.
| Original language | English |
|---|---|
| Pages (from-to) | 915-923 |
| Number of pages | 9 |
| Journal | AIDS |
| Volume | 6 |
| Issue number | 9 |
| State | Published - Sep 1 1992 |
Fingerprint
Keywords
- AIDS
- Central nervous system
- Detection
- Explant
- HIV-1
- Immunohistochemistry
- In situ hybridization
- Opportunistic infections
- Virus isolation
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
Cite this
HIV-1 in postmortem brain tissue from patients with AIDS : A comparison of different detection techniques. / Shapshak, Paul; Yoshioka, Masaru; Sun, Nora C J; Nelson, Stephen J.; Rhodes, Roy H.; Schiller, Paul C; Resnick, Lionel; Shah, Syed M.; Svenningsson, Anders; Imagawa, David T.
In: AIDS, Vol. 6, No. 9, 01.09.1992, p. 915-923.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - HIV-1 in postmortem brain tissue from patients with AIDS
T2 - A comparison of different detection techniques
AU - Shapshak, Paul
AU - Yoshioka, Masaru
AU - Sun, Nora C J
AU - Nelson, Stephen J.
AU - Rhodes, Roy H.
AU - Schiller, Paul C
AU - Resnick, Lionel
AU - Shah, Syed M.
AU - Svenningsson, Anders
AU - Imagawa, David T.
PY - 1992/9/1
Y1 - 1992/9/1
N2 - Objective: The presence of HIV-1 in postmortem brain tissue from 31 patients with AIDS and 12 HIV-1-negative controls was investigated. Design: Most laboratories have access to the methods used. We readily applied in situ hybridization and immunohistochemistry to archival formalin-fixed paraffin-embedded (FFPE) brain specimens. Methods: The techniques used to detect HIV-1 were explant culture, in situ hybridization with 35S-labeled polymerase (pol) gene riboprobes and immunohistochemistry with monoclonal antibody to gp41. Results: HIV-1 was isolated from explant cultures in 13 out of 20 (65%) patients, whereas HIV-1-infected cells were detected in FFPE brain tissue from nine out of 26 (35%) patients examined by in situ hybridization and in seven out of 26 (27%) patients examined by immunohistochemistry. Conclusions: Although the isolation technique was the most sensitive of the three techniques tested, infected cells may be identified with in situ hybridization in conjunction with immunohistochemistry.
AB - Objective: The presence of HIV-1 in postmortem brain tissue from 31 patients with AIDS and 12 HIV-1-negative controls was investigated. Design: Most laboratories have access to the methods used. We readily applied in situ hybridization and immunohistochemistry to archival formalin-fixed paraffin-embedded (FFPE) brain specimens. Methods: The techniques used to detect HIV-1 were explant culture, in situ hybridization with 35S-labeled polymerase (pol) gene riboprobes and immunohistochemistry with monoclonal antibody to gp41. Results: HIV-1 was isolated from explant cultures in 13 out of 20 (65%) patients, whereas HIV-1-infected cells were detected in FFPE brain tissue from nine out of 26 (35%) patients examined by in situ hybridization and in seven out of 26 (27%) patients examined by immunohistochemistry. Conclusions: Although the isolation technique was the most sensitive of the three techniques tested, infected cells may be identified with in situ hybridization in conjunction with immunohistochemistry.
KW - AIDS
KW - Central nervous system
KW - Detection
KW - Explant
KW - HIV-1
KW - Immunohistochemistry
KW - In situ hybridization
KW - Opportunistic infections
KW - Virus isolation
UR - http://www.scopus.com/inward/record.url?scp=0026649587&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026649587&partnerID=8YFLogxK
M3 - Article
C2 - 1326996
AN - SCOPUS:0026649587
VL - 6
SP - 915
EP - 923
JO - AIDS
JF - AIDS
SN - 0269-9370
IS - 9
ER -