HIV-1 in postmortem brain tissue from patients with AIDS: A comparison of different detection techniques

P. Shapshak, M. Yoshioka, N. C.J. Sun, S. J. Nelson, R. H. Rhodes, P. Schiller, L. Resnick, S. M. Shah, A. Svenningsson, D. T. Imagawa

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Objective: The presence of HIV-1 in postmortem brain tissue from 31 patients with AIDS and 12 HIV-1-negative controls was investigated. Design: Most laboratories have access to the methods used. We readily applied in situ hybridization and immunohistochemistry to archival formalin-fixed paraffin-embedded (FFPE) brain specimens. Methods: The techniques used to detect HIV-1 were explant culture, in situ hybridization with 35S-labeled polymerase (pol) gene riboprobes and immunohistochemistry with monoclonal antibody to gp41. Results: HIV-1 was isolated from explant cultures in 13 out of 20 (65%) patients, whereas HIV-1-infected cells were detected in FFPE brain tissue from nine out of 26 (35%) patients examined by in situ hybridization and in seven out of 26 (27%) patients examined by immunohistochemistry. Conclusions: Although the isolation technique was the most sensitive of the three techniques tested, infected cells may be identified with in situ hybridization in conjunction with immunohistochemistry.

Original languageEnglish (US)
Pages (from-to)915-923
Number of pages9
JournalAIDS
Volume6
Issue number9
DOIs
StatePublished - 1992

Keywords

  • AIDS
  • Central nervous system
  • Detection
  • Explant
  • HIV-1
  • Immunohistochemistry
  • In situ hybridization
  • Opportunistic infections
  • Virus isolation

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Shapshak, P., Yoshioka, M., Sun, N. C. J., Nelson, S. J., Rhodes, R. H., Schiller, P., Resnick, L., Shah, S. M., Svenningsson, A., & Imagawa, D. T. (1992). HIV-1 in postmortem brain tissue from patients with AIDS: A comparison of different detection techniques. AIDS, 6(9), 915-923. https://doi.org/10.1097/00002030-199209000-00002