Abstract
Objective: The presence of HIV-1 in postmortem brain tissue from 31 patients with AIDS and 12 HIV-1-negative controls was investigated. Design: Most laboratories have access to the methods used. We readily applied in situ hybridization and immunohistochemistry to archival formalin-fixed paraffin-embedded (FFPE) brain specimens. Methods: The techniques used to detect HIV-1 were explant culture, in situ hybridization with 35S-labeled polymerase (pol) gene riboprobes and immunohistochemistry with monoclonal antibody to gp41. Results: HIV-1 was isolated from explant cultures in 13 out of 20 (65%) patients, whereas HIV-1-infected cells were detected in FFPE brain tissue from nine out of 26 (35%) patients examined by in situ hybridization and in seven out of 26 (27%) patients examined by immunohistochemistry. Conclusions: Although the isolation technique was the most sensitive of the three techniques tested, infected cells may be identified with in situ hybridization in conjunction with immunohistochemistry.
Original language | English (US) |
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Pages (from-to) | 915-923 |
Number of pages | 9 |
Journal | AIDS |
Volume | 6 |
Issue number | 9 |
DOIs | |
State | Published - 1992 |
Keywords
- AIDS
- Central nervous system
- Detection
- Explant
- HIV-1
- Immunohistochemistry
- In situ hybridization
- Opportunistic infections
- Virus isolation
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology