HIV-1 gp120 blocks jacalin-induced proliferative response in CD4+ T cells: Jacalin as a useful surrogate marker for qualitative and quantitative deficiency of CD4+ T cells in HIV-1 infection

Seetha M. Lakshmi Tamma, Naoki Oyaizu, Thomas W. McCloskey, Vaniambadi S. Kalyanaraman, Savita G Pahwa

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Jacalin is a plant lectin that induces mitogenic responses selectively in CD4+ T lymphocytes and has been shown to block infection by the human immunodeficiency virus type 1 (HIV-1) in a T lymphoid cell line, but the relationship of jacalin to the HIV envelope glycoprotein gp120 in its interaction with the CD4 molecule is unclear. Here we demonstrate that pretreatment of normal T cells with native HIV-1 gp120 impairs their ability to proliferate and secrete IL-2 in response to jacalin. This effect was not observed with deglycosylated gp120, which fails to bind to CD4 molecule, or with gp120 that has been premixed with soluble CD4. Flow cytometric studies and Western blotting analysis indicated that gp120 and jacalin compete with each other in binding to CD4 molecules. In HIV-infected patients, proliferative responses of PBMC in response to jacalin were found to correlate quantitatively with percentages of CD4+ T cells but also showed a qualitative defect in comparison to healthy volunteers based on responses that were correlated for CD4+ T cell numbers. These findings suggest that (i) gp120 and jacalin compete with each other for CD4 binding and (ii) jacalin might be a useful surrogate marker for quantitative as well as qualitative deficiency of CD4+ T Cells in HIV-1 infection.

Original languageEnglish
Pages (from-to)290-297
Number of pages8
JournalClinical Immunology and Immunopathology
Volume80
Issue number3 II
StatePublished - Dec 1 1996
Externally publishedYes

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Virus Diseases
HIV-1
Biomarkers
T-Lymphocytes
CD4 Antigens
HIV Envelope Protein gp120
Plant Lectins
jacalin
Interleukin-2
Healthy Volunteers
Cell Count
Western Blotting
HIV
Lymphocytes
Cell Line
Infection

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Pathology and Forensic Medicine

Cite this

HIV-1 gp120 blocks jacalin-induced proliferative response in CD4+ T cells : Jacalin as a useful surrogate marker for qualitative and quantitative deficiency of CD4+ T cells in HIV-1 infection. / Lakshmi Tamma, Seetha M.; Oyaizu, Naoki; McCloskey, Thomas W.; Kalyanaraman, Vaniambadi S.; Pahwa, Savita G.

In: Clinical Immunology and Immunopathology, Vol. 80, No. 3 II, 01.12.1996, p. 290-297.

Research output: Contribution to journalArticle

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abstract = "Jacalin is a plant lectin that induces mitogenic responses selectively in CD4+ T lymphocytes and has been shown to block infection by the human immunodeficiency virus type 1 (HIV-1) in a T lymphoid cell line, but the relationship of jacalin to the HIV envelope glycoprotein gp120 in its interaction with the CD4 molecule is unclear. Here we demonstrate that pretreatment of normal T cells with native HIV-1 gp120 impairs their ability to proliferate and secrete IL-2 in response to jacalin. This effect was not observed with deglycosylated gp120, which fails to bind to CD4 molecule, or with gp120 that has been premixed with soluble CD4. Flow cytometric studies and Western blotting analysis indicated that gp120 and jacalin compete with each other in binding to CD4 molecules. In HIV-infected patients, proliferative responses of PBMC in response to jacalin were found to correlate quantitatively with percentages of CD4+ T cells but also showed a qualitative defect in comparison to healthy volunteers based on responses that were correlated for CD4+ T cell numbers. These findings suggest that (i) gp120 and jacalin compete with each other for CD4 binding and (ii) jacalin might be a useful surrogate marker for quantitative as well as qualitative deficiency of CD4+ T Cells in HIV-1 infection.",
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