Histone modifications silence the GATA transcription factor genes in ovarian cancer

C. Caslini, C. D. Capo-Chichi, I. H. Roland, E. Nicolas, A. T. Yeung, Xiangxi Xu

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

Altered expression of GATA factors was found and proposed as the underlying mechanism for dedifferentiation in ovarian carcinogenesis. In particular, GATA6 is lost or excluded from the nucleus in 85% of ovarian tumors and GATA4 expression is absent in majority of ovarian cancer cell lines. Here, we evaluated their DNA and histone epigenetic modifications in five ovarian epithelial and carcinoma cell lines (human 'immortalized' ovarian surface epithelium (HIO)-117, HIO-114, A2780, SKOV3 and ES2). GATA4 and GATA6 gene silencing was found to correlate with hypoacetylation of histones H3 and H4 and loss of histone H3/lysine K4 tri-methylation at their promoters in all lines. Conversely, histone H3/lysine K9 di-methylation and HP1γ association were not observed, excluding reorganization of GATA genes into heterochromatic structures. The histone deacetylase inhibitor trichostatin A, but not the DNA methylation inhibitor 5′-aza-2′-deoxycytidine, re-established the expression of GATA4 and/or GATA6 in A2780 and HIO-114 cells, correlating with increased histone H3 and H4 acetylation, histone H3 lysine K4 methylation and DNase I sensitivity at the promoters. Therefore, altered histone modification of the promoter loci is one mechanism responsible for the silencing of GATA transcription factors and the subsequent loss of a target gene, the tumor suppressor Disabled-2, in ovarian carcinogenesis.

Original languageEnglish
Pages (from-to)5446-5461
Number of pages16
JournalOncogene
Volume25
Issue number39
DOIs
StatePublished - Aug 31 2006
Externally publishedYes

Fingerprint

Histone Code
GATA Transcription Factors
Ovarian Neoplasms
Histones
Genes
Methylation
Lysine
decitabine
trichostatin A
Carcinogenesis
Cell Line
Histone Deacetylase Inhibitors
Deoxyribonuclease I
Gene Silencing
DNA Methylation
Acetylation
Tumor Suppressor Genes
Epigenomics
Epithelium
Epithelial Cells

Keywords

  • Chromatin
  • Disabled-2 (DAB2)
  • GATA transcription factors
  • Ovarian carcinomas
  • Ovarian epithelial cells
  • Transcription repression

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

Caslini, C., Capo-Chichi, C. D., Roland, I. H., Nicolas, E., Yeung, A. T., & Xu, X. (2006). Histone modifications silence the GATA transcription factor genes in ovarian cancer. Oncogene, 25(39), 5446-5461. https://doi.org/10.1038/sj.onc.1209533

Histone modifications silence the GATA transcription factor genes in ovarian cancer. / Caslini, C.; Capo-Chichi, C. D.; Roland, I. H.; Nicolas, E.; Yeung, A. T.; Xu, Xiangxi.

In: Oncogene, Vol. 25, No. 39, 31.08.2006, p. 5446-5461.

Research output: Contribution to journalArticle

Caslini, C, Capo-Chichi, CD, Roland, IH, Nicolas, E, Yeung, AT & Xu, X 2006, 'Histone modifications silence the GATA transcription factor genes in ovarian cancer', Oncogene, vol. 25, no. 39, pp. 5446-5461. https://doi.org/10.1038/sj.onc.1209533
Caslini C, Capo-Chichi CD, Roland IH, Nicolas E, Yeung AT, Xu X. Histone modifications silence the GATA transcription factor genes in ovarian cancer. Oncogene. 2006 Aug 31;25(39):5446-5461. https://doi.org/10.1038/sj.onc.1209533
Caslini, C. ; Capo-Chichi, C. D. ; Roland, I. H. ; Nicolas, E. ; Yeung, A. T. ; Xu, Xiangxi. / Histone modifications silence the GATA transcription factor genes in ovarian cancer. In: Oncogene. 2006 ; Vol. 25, No. 39. pp. 5446-5461.
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