Histone H3 K4 demethylation during activation and attenuation of GAL1 tanscription in Saccharomyces cerevisiae

Kristin Ingvarsdottir, Chris Edwards, Min Gyu Lee, Jung Shin Lee, David C. Schultz, Ali Shilatifard, Ramin Shiekhattar, Shelley L. Berger

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

In mammalian cells, histone lysine demethylation is carried out by two classes of enzymes, the LSD1/BHC110 class and the jumonji class. The enzymes of the jumonji class in the yeast Saccharomyces cerevisiae have recently also been shown to have lysine demethylation activity. Here we report that the protein encoded by YJR119c (termed KDM5), coding for one of five predicted jumonji domain proteins in yeast, specifically demethylates trimethylated histone H3 lysine 4 (H3K4me3), H3K4me2, and H3K4me1 in vitro. We found that loss of KDM5 increased mono-, di-, and trimethylation of lysine 4 during activation of the GAL1 gene. Interestingly, cells deleted of KDM5 also displayed a delayed reduction of K4me3 upon reestablishment of GAL1 repression. These results indicate that K4 demethylation has two roles at GAL1, first to establish appropriate levels of K4 methylation during gene activation and second to remove K4 trimethylation during the attenuation phase of transcription. Thus, analysis of lysine demethylation in yeast provides new insight into the physiological roles of jumonji demethylase enzymes.

Original languageEnglish (US)
Pages (from-to)7856-7864
Number of pages9
JournalMolecular and Cellular Biology
Volume27
Issue number22
DOIs
StatePublished - Nov 2007
Externally publishedYes

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Histones
Lysine
Saccharomyces cerevisiae
Transcriptional Activation
Enzymes
Yeasts
Fungal Proteins
Methylation
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Ingvarsdottir, K., Edwards, C., Lee, M. G., Lee, J. S., Schultz, D. C., Shilatifard, A., ... Berger, S. L. (2007). Histone H3 K4 demethylation during activation and attenuation of GAL1 tanscription in Saccharomyces cerevisiae. Molecular and Cellular Biology, 27(22), 7856-7864. https://doi.org/10.1128/MCB.00801-07

Histone H3 K4 demethylation during activation and attenuation of GAL1 tanscription in Saccharomyces cerevisiae. / Ingvarsdottir, Kristin; Edwards, Chris; Lee, Min Gyu; Lee, Jung Shin; Schultz, David C.; Shilatifard, Ali; Shiekhattar, Ramin; Berger, Shelley L.

In: Molecular and Cellular Biology, Vol. 27, No. 22, 11.2007, p. 7856-7864.

Research output: Contribution to journalArticle

Ingvarsdottir, K, Edwards, C, Lee, MG, Lee, JS, Schultz, DC, Shilatifard, A, Shiekhattar, R & Berger, SL 2007, 'Histone H3 K4 demethylation during activation and attenuation of GAL1 tanscription in Saccharomyces cerevisiae', Molecular and Cellular Biology, vol. 27, no. 22, pp. 7856-7864. https://doi.org/10.1128/MCB.00801-07
Ingvarsdottir, Kristin ; Edwards, Chris ; Lee, Min Gyu ; Lee, Jung Shin ; Schultz, David C. ; Shilatifard, Ali ; Shiekhattar, Ramin ; Berger, Shelley L. / Histone H3 K4 demethylation during activation and attenuation of GAL1 tanscription in Saccharomyces cerevisiae. In: Molecular and Cellular Biology. 2007 ; Vol. 27, No. 22. pp. 7856-7864.
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