Histological, immunohistochemical, and ultrastructural features of a rat medullary thyroid carcinoma transfected with a corticotropin-releasing hormone cDNA expression vector

Meora Feinmesser, Sylvia L. Asa, Kaiman Kovacs, Bernard A. Roos, Malcolm J. Low

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Abstract

Clonal cell lines producing corticotropin-releasing hormone (CRH) have been generated by transfection of the W2 rat medullary thyroid carcinoma (MTC) cell with a CRH-encoding CMV/ SV40 expression vector. Here, we report the morphological, immunohistochemical, and ultrastructural features of rat tumors derived by implantation of CRH-producing W2CRH-7 cells and compare them with non-CRH-producing W2 MTCs. Both types of tumors grew rapidly and consisted of sheets and nests of pleomorphic cells infiltrating adjacent adipose tissue. Immunohistochemistry revealed CRH in only W2CRH-7 tumors; scattered cells in these tumors also were immunoreactive for chromogranin and for vasoactive intestinal peptide. Otherwise, the two tumor types exhibited similar profiles of various neuroendocrine markers, including neuron-specific enolase, synaptophysin, calcitonin, and somatostatin. Ultrastructurally, the tumors contained abundant dilated rough endoplasmic reticulum, a prominent Golgi apparatus, and numerous lysosomes. Very few secretory granules were noted in the W2 tumors; by contrast, secretory granules, although still not numerous in the majority of W2CRH-7 cells, were more abundant in scattered cells of those tumors. The positive immunostaining for CRH is consistent with the observations of increased plasma CRH and pituitary-adrenal activation induced by these transplanted tumors. This system provides a valuable model for CRH excess mimicking tumoral CRH-dependent Cushing's syndrome in human patients.

Original languageEnglish
Pages (from-to)39-46
Number of pages8
JournalEndocrine Pathology
Volume3
Issue number1
DOIs
StatePublished - Mar 1 1992
Externally publishedYes

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Corticotropin-Releasing Hormone
Complementary DNA
Neoplasms
Secretory Vesicles
Chromogranins
Medullary Thyroid cancer
Synaptophysin
Cushing Syndrome
Phosphopyruvate Hydratase
Rough Endoplasmic Reticulum
Vasoactive Intestinal Peptide
Calcitonin
Golgi Apparatus
Lysosomes
Somatostatin
Transfection
Adipose Tissue
Immunohistochemistry
Hormones
Cell Line

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Cite this

Histological, immunohistochemical, and ultrastructural features of a rat medullary thyroid carcinoma transfected with a corticotropin-releasing hormone cDNA expression vector. / Feinmesser, Meora; Asa, Sylvia L.; Kovacs, Kaiman; Roos, Bernard A.; Low, Malcolm J.

In: Endocrine Pathology, Vol. 3, No. 1, 01.03.1992, p. 39-46.

Research output: Contribution to journalArticle

Feinmesser, Meora ; Asa, Sylvia L. ; Kovacs, Kaiman ; Roos, Bernard A. ; Low, Malcolm J. / Histological, immunohistochemical, and ultrastructural features of a rat medullary thyroid carcinoma transfected with a corticotropin-releasing hormone cDNA expression vector. In: Endocrine Pathology. 1992 ; Vol. 3, No. 1. pp. 39-46.
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