Histologic, ultrastructural, and immunomicroscopic findings in 96 one hour human renal allograft biopsy specimens. Immunologic and clinical significance

R. Valenzuela, S. A. Hamway, S. D. Deodhar, W. E. Braun, L. H. Banowsky, M. O. Magnusson, D. G. Osborne

Research output: Contribution to journalArticle

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Abstract

Predicting the outcome of human renal allografts based on studies of one hour biopsy specimens is still controversial. We have tried to correlate histologic, ultrastructural, and immunofluorescence findings in 96 one hour biopsy specimens with histocompatibility matching, the presence of preformed antibodies, allograft ischemia and preservation times, the donor's age, the original renal disease, and allograft source, function, and survival. Ultrastructurally, 22 allografts had fibrin deposits in glomerular loops. There was a significant correlation between this finding and poor allograft function (p < 0.01), cold ischemia time (p < 0.02), and cadaveric allograft source (p < 0.01). Sixteen allografts showed epithelial cell detachment from tubular basement membranes. This finding correlated with cadaveric allograft source (p < 0.01). Many other morphologic changes were evaluated by both light and electron microscopy, but they did not bear any significant relationship to any of the aforementioned clinical parameters. Of 30 biopsy specimens studied by direct immunofluorescence, 11 showed positive findings (immunoglobulins or C3) in either glomeruli, vessels, or both. There was no significant correlation between these findings and the clinical parameters.

Original languageEnglish
Pages (from-to)187-195
Number of pages9
JournalHuman Pathology
Volume11
Issue number2
DOIs
StatePublished - Jul 18 1980
Externally publishedYes

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Allografts
Kidney
Biopsy
Cold Ischemia
Direct Fluorescent Antibody Technique
Histocompatibility
Fibrin
Basement Membrane
Fluorescent Antibody Technique
Immunoglobulins
Electron Microscopy
Ischemia
Epithelial Cells
Light
Survival
Antibodies

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Histologic, ultrastructural, and immunomicroscopic findings in 96 one hour human renal allograft biopsy specimens. Immunologic and clinical significance. / Valenzuela, R.; Hamway, S. A.; Deodhar, S. D.; Braun, W. E.; Banowsky, L. H.; Magnusson, M. O.; Osborne, D. G.

In: Human Pathology, Vol. 11, No. 2, 18.07.1980, p. 187-195.

Research output: Contribution to journalArticle

Valenzuela, R. ; Hamway, S. A. ; Deodhar, S. D. ; Braun, W. E. ; Banowsky, L. H. ; Magnusson, M. O. ; Osborne, D. G. / Histologic, ultrastructural, and immunomicroscopic findings in 96 one hour human renal allograft biopsy specimens. Immunologic and clinical significance. In: Human Pathology. 1980 ; Vol. 11, No. 2. pp. 187-195.
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