TY - JOUR
T1 - Hirschsprung's disease
T2 - A comparison of the nervous control of ganglionic and aganglionic smooth muscle in vitro
AU - Larsson, Lars Torsten
AU - Malmfors, Gerhard
AU - Wahlestedt, Claes
AU - Leander, Stefan
AU - Håkanson, Rolf
N1 - Funding Information:
From the Departments of Pediatric Surgery and Pharmacology, University of Lund, Sweden. Supported by grants from the Swedish Medical Research Council {04X-1007). Address reprint requests to Gerhard Malmfors, MD, PhD, Department of Pediatric Surgery, Lunds lasarett, S-22l 85 Land, Sweden. 9 by Grune & Stratton, Inc. 0022-3468/87/2205-0010503.00/0
PY - 1987/5
Y1 - 1987/5
N2 - Specimens from aganglionic (constricted) and ganglionic (dilated) gut were obtained from nine patients with Hirschsprung's disease. Trensmural nerve stimulation of ganglionic smooth muscle in vitro evoked an initial relaxation followed by a contraction. This contraction was reduced but not abolished by atropine and it was further reduced by substance P antagonists. Guanethidine did not affect the electrically evoked responses. In aganglionic smooth muscle, an atropine-sensitive contraction but no initial relaxation was registered. Tetrodotoxin abolished all responses to electrical stimulation in both ganglionic and aganglionic specimens. Application of carbachol or substance P produced contraction and the adrenergic agonist isoprenaline or vasoactive intestinal peptide produced relaxation in ganglionic as well as aganglionic specimens. Two other gut neuropeptides, neuropeptide Y and galanin, were without effect. The results do not indicate a different receptor set up in ganglionic v aganglionic gut. The results are compatible with a lack of noncholinergic nonadrenergic inhibitory neurons in the aganglionic gut.
AB - Specimens from aganglionic (constricted) and ganglionic (dilated) gut were obtained from nine patients with Hirschsprung's disease. Trensmural nerve stimulation of ganglionic smooth muscle in vitro evoked an initial relaxation followed by a contraction. This contraction was reduced but not abolished by atropine and it was further reduced by substance P antagonists. Guanethidine did not affect the electrically evoked responses. In aganglionic smooth muscle, an atropine-sensitive contraction but no initial relaxation was registered. Tetrodotoxin abolished all responses to electrical stimulation in both ganglionic and aganglionic specimens. Application of carbachol or substance P produced contraction and the adrenergic agonist isoprenaline or vasoactive intestinal peptide produced relaxation in ganglionic as well as aganglionic specimens. Two other gut neuropeptides, neuropeptide Y and galanin, were without effect. The results do not indicate a different receptor set up in ganglionic v aganglionic gut. The results are compatible with a lack of noncholinergic nonadrenergic inhibitory neurons in the aganglionic gut.
KW - adrenergic mechanisms
KW - cholinergic mechanims
KW - galanin
KW - Hirschsprung's disease
KW - neuropeptide Y (NPY)
KW - neuropeptides
KW - substance P (SP)
KW - vasoactive intestinal peptide (VIP)
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U2 - 10.1016/S0022-3468(87)80263-4
DO - 10.1016/S0022-3468(87)80263-4
M3 - Article
C2 - 3585666
AN - SCOPUS:0023212005
VL - 22
SP - 431
EP - 435
JO - Journal of Pediatric Surgery
JF - Journal of Pediatric Surgery
SN - 0022-3468
IS - 5
ER -