Hirschsprung's disease: A comparison of the nervous control of ganglionic and aganglionic smooth muscle in vitro

Lars Torsten Larsson, Gerhard Malmfors, Claes Wahlestedt, Stefan Leander, Rolf Håkanson

Research output: Contribution to journalArticlepeer-review

19 Scopus citations


Specimens from aganglionic (constricted) and ganglionic (dilated) gut were obtained from nine patients with Hirschsprung's disease. Trensmural nerve stimulation of ganglionic smooth muscle in vitro evoked an initial relaxation followed by a contraction. This contraction was reduced but not abolished by atropine and it was further reduced by substance P antagonists. Guanethidine did not affect the electrically evoked responses. In aganglionic smooth muscle, an atropine-sensitive contraction but no initial relaxation was registered. Tetrodotoxin abolished all responses to electrical stimulation in both ganglionic and aganglionic specimens. Application of carbachol or substance P produced contraction and the adrenergic agonist isoprenaline or vasoactive intestinal peptide produced relaxation in ganglionic as well as aganglionic specimens. Two other gut neuropeptides, neuropeptide Y and galanin, were without effect. The results do not indicate a different receptor set up in ganglionic v aganglionic gut. The results are compatible with a lack of noncholinergic nonadrenergic inhibitory neurons in the aganglionic gut.

Original languageEnglish (US)
Pages (from-to)431-435
Number of pages5
JournalJournal of Pediatric Surgery
Issue number5
StatePublished - May 1987
Externally publishedYes


  • adrenergic mechanisms
  • cholinergic mechanims
  • galanin
  • Hirschsprung's disease
  • neuropeptide Y (NPY)
  • neuropeptides
  • substance P (SP)
  • vasoactive intestinal peptide (VIP)

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Surgery


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