Hirschsprung's disease

A comparison of the nervous control of ganglionic and aganglionic smooth muscle in vitro

Lars Torsten Larsson, Gerhard Malmfors, Claes R Wahlestedt, Stefan Leander, Rolf Håkanson

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Specimens from aganglionic (constricted) and ganglionic (dilated) gut were obtained from nine patients with Hirschsprung's disease. Trensmural nerve stimulation of ganglionic smooth muscle in vitro evoked an initial relaxation followed by a contraction. This contraction was reduced but not abolished by atropine and it was further reduced by substance P antagonists. Guanethidine did not affect the electrically evoked responses. In aganglionic smooth muscle, an atropine-sensitive contraction but no initial relaxation was registered. Tetrodotoxin abolished all responses to electrical stimulation in both ganglionic and aganglionic specimens. Application of carbachol or substance P produced contraction and the adrenergic agonist isoprenaline or vasoactive intestinal peptide produced relaxation in ganglionic as well as aganglionic specimens. Two other gut neuropeptides, neuropeptide Y and galanin, were without effect. The results do not indicate a different receptor set up in ganglionic v aganglionic gut. The results are compatible with a lack of noncholinergic nonadrenergic inhibitory neurons in the aganglionic gut.

Original languageEnglish
Pages (from-to)431-435
Number of pages5
JournalJournal of Pediatric Surgery
Volume22
Issue number5
DOIs
StatePublished - Jan 1 1987
Externally publishedYes

Fingerprint

Hirschsprung Disease
Substance P
Atropine
Smooth Muscle
Galanin
Guanethidine
Adrenergic Agonists
Neuropeptide Y
Vasoactive Intestinal Peptide
Tetrodotoxin
Carbachol
Neuropeptides
Isoproterenol
Electric Stimulation
Neurons
In Vitro Techniques

Keywords

  • adrenergic mechanisms
  • cholinergic mechanims
  • galanin
  • Hirschsprung's disease
  • neuropeptide Y (NPY)
  • neuropeptides
  • substance P (SP)
  • vasoactive intestinal peptide (VIP)

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Surgery

Cite this

Hirschsprung's disease : A comparison of the nervous control of ganglionic and aganglionic smooth muscle in vitro. / Larsson, Lars Torsten; Malmfors, Gerhard; Wahlestedt, Claes R; Leander, Stefan; Håkanson, Rolf.

In: Journal of Pediatric Surgery, Vol. 22, No. 5, 01.01.1987, p. 431-435.

Research output: Contribution to journalArticle

Larsson, Lars Torsten ; Malmfors, Gerhard ; Wahlestedt, Claes R ; Leander, Stefan ; Håkanson, Rolf. / Hirschsprung's disease : A comparison of the nervous control of ganglionic and aganglionic smooth muscle in vitro. In: Journal of Pediatric Surgery. 1987 ; Vol. 22, No. 5. pp. 431-435.
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