Hippocampal stem cells differentiate into excitatory and inhibitory neurons

Carlos Vicario-Abejón, Carlos Collin, Pantelis Tsoulfas, Ronald D G McKay

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

Stem cell technology promises new and rapid advances in cell therapy and drug discovery. Clearly, the value of this approach will be limited by the differentiated functions displayed by the progeny of stem cells. The foetal and adult central nervous system (CNS) harbour stem cells that can be expanded in vitro and differentiate into immature neurons and glia. Surprisingly, we do not know if neurons derived from stem cells form synapses, a definitive feature of neuronal function. Neuronal differentiation is a complex process and in this paper we establish conditions that permit extensive maturation of neurons in the presence of neurotrophins. These conditions permit the differentiation of rat hippocampal stem cells into both excitatory (glutamatergic) and inhibitory (GABAergic) neurons. The proportion of excitatory and inhibitory synapses was strongly influenced by specific neurotrophins, and these responses reflect the region of origin of the stem cells in the brain. These data show that stem cells can be used to study mechanisms of excitation and inhibition in the nervous system.

Original languageEnglish
Pages (from-to)677-688
Number of pages12
JournalEuropean Journal of Neuroscience
Volume12
Issue number2
DOIs
StatePublished - Mar 22 2000

Fingerprint

Stem Cells
Neurons
Nerve Growth Factors
Synapses
GABAergic Neurons
Drug Discovery
Cell- and Tissue-Based Therapy
Neuroglia
Nervous System
Central Nervous System
Technology
Brain

Keywords

  • BDNF
  • GABAergic
  • Glutamatergic
  • NT-3
  • Rat

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Hippocampal stem cells differentiate into excitatory and inhibitory neurons. / Vicario-Abejón, Carlos; Collin, Carlos; Tsoulfas, Pantelis; McKay, Ronald D G.

In: European Journal of Neuroscience, Vol. 12, No. 2, 22.03.2000, p. 677-688.

Research output: Contribution to journalArticle

Vicario-Abejón, Carlos ; Collin, Carlos ; Tsoulfas, Pantelis ; McKay, Ronald D G. / Hippocampal stem cells differentiate into excitatory and inhibitory neurons. In: European Journal of Neuroscience. 2000 ; Vol. 12, No. 2. pp. 677-688.
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