Highly scalable, uniform, and sensitive biosensors based on top-down indium oxide nanoribbons and electronic enzyme-linked immunosorbent assay

Noppadol Aroonyadet, Xiaoli Wang, Yan Song, Haitian Chen, Richard J Cote, Mark E. Thompson, Ram Datar, Chongwu Zhou

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Nanostructure field-effect transistor (FET) biosensors have shown great promise for ultra sensitive biomolecular detection. Top-down assembly of these sensors increases scalability and device uniformity but faces fabrication challenges in achieving the small dimensions needed for sensitivity. We report top-down fabricated indium oxide (In2O3) nanoribbon FET biosensors using highly scalable radio frequency (RF) sputtering to create uniform channel thicknesses ranging from 50 to 10 nm. We combine this scalable sensing platform with amplification from electronic enzyme-linked immunosorbent assay (ELISA) to achieve high sensitivity to target analytes such as streptavidin and human immunodeficiency virus type 1 (HIV-1) p24 proteins. Our approach circumvents Debye screening in ionic solutions and detects p24 protein at 20 fg/mL (about 250 viruses/mL or about 3 orders of magnitude lower than commercial ELISA) with a 35% conduction change in human serum. The In2O3 nanoribbon biosensors have 100% device yield and use a simple 2 mask photolithography process. The electrical properties of 50 In2O3 nanoribbon FETs showed good uniformity in on-state current, on/off current ratio, mobility, and threshold voltage. In addition, the sensors show excellent pH sensitivity over a broad range (pH 4 to 9) as well as over the physiological-related pH range (pH 6.8 to 8.2). With the demonstrated sensitivity, scalability, and uniformity, the In2O3 nanoribbon sensor platform makes great progress toward clinical testing, such as for early diagnosis of acquired immunodeficiency syndrome (AIDS).

Original languageEnglish (US)
Pages (from-to)1943-1951
Number of pages9
JournalNano Letters
Volume15
Issue number3
DOIs
StatePublished - Mar 11 2015

Fingerprint

Nanoribbons
Immunosorbents
Carbon Nanotubes
bioinstrumentation
Biosensors
indium oxides
Indium
enzymes
Assays
Enzymes
Field effect transistors
Oxides
field effect transistors
sensitivity
Viruses
electronics
Scalability
sensors
Sensors
platforms

Keywords

  • biosensor
  • electronic ELISA
  • indium oxide
  • nanoribbon
  • top-down fabrication

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Mechanical Engineering

Cite this

Highly scalable, uniform, and sensitive biosensors based on top-down indium oxide nanoribbons and electronic enzyme-linked immunosorbent assay. / Aroonyadet, Noppadol; Wang, Xiaoli; Song, Yan; Chen, Haitian; Cote, Richard J; Thompson, Mark E.; Datar, Ram; Zhou, Chongwu.

In: Nano Letters, Vol. 15, No. 3, 11.03.2015, p. 1943-1951.

Research output: Contribution to journalArticle

Aroonyadet, Noppadol ; Wang, Xiaoli ; Song, Yan ; Chen, Haitian ; Cote, Richard J ; Thompson, Mark E. ; Datar, Ram ; Zhou, Chongwu. / Highly scalable, uniform, and sensitive biosensors based on top-down indium oxide nanoribbons and electronic enzyme-linked immunosorbent assay. In: Nano Letters. 2015 ; Vol. 15, No. 3. pp. 1943-1951.
@article{f1c9a8cf66c6437da2e524efc7f20329,
title = "Highly scalable, uniform, and sensitive biosensors based on top-down indium oxide nanoribbons and electronic enzyme-linked immunosorbent assay",
abstract = "Nanostructure field-effect transistor (FET) biosensors have shown great promise for ultra sensitive biomolecular detection. Top-down assembly of these sensors increases scalability and device uniformity but faces fabrication challenges in achieving the small dimensions needed for sensitivity. We report top-down fabricated indium oxide (In2O3) nanoribbon FET biosensors using highly scalable radio frequency (RF) sputtering to create uniform channel thicknesses ranging from 50 to 10 nm. We combine this scalable sensing platform with amplification from electronic enzyme-linked immunosorbent assay (ELISA) to achieve high sensitivity to target analytes such as streptavidin and human immunodeficiency virus type 1 (HIV-1) p24 proteins. Our approach circumvents Debye screening in ionic solutions and detects p24 protein at 20 fg/mL (about 250 viruses/mL or about 3 orders of magnitude lower than commercial ELISA) with a 35{\%} conduction change in human serum. The In2O3 nanoribbon biosensors have 100{\%} device yield and use a simple 2 mask photolithography process. The electrical properties of 50 In2O3 nanoribbon FETs showed good uniformity in on-state current, on/off current ratio, mobility, and threshold voltage. In addition, the sensors show excellent pH sensitivity over a broad range (pH 4 to 9) as well as over the physiological-related pH range (pH 6.8 to 8.2). With the demonstrated sensitivity, scalability, and uniformity, the In2O3 nanoribbon sensor platform makes great progress toward clinical testing, such as for early diagnosis of acquired immunodeficiency syndrome (AIDS).",
keywords = "biosensor, electronic ELISA, indium oxide, nanoribbon, top-down fabrication",
author = "Noppadol Aroonyadet and Xiaoli Wang and Yan Song and Haitian Chen and Cote, {Richard J} and Thompson, {Mark E.} and Ram Datar and Chongwu Zhou",
year = "2015",
month = "3",
day = "11",
doi = "10.1021/nl5047889",
language = "English (US)",
volume = "15",
pages = "1943--1951",
journal = "Nano Letters",
issn = "1530-6984",
publisher = "American Chemical Society",
number = "3",

}

TY - JOUR

T1 - Highly scalable, uniform, and sensitive biosensors based on top-down indium oxide nanoribbons and electronic enzyme-linked immunosorbent assay

AU - Aroonyadet, Noppadol

AU - Wang, Xiaoli

AU - Song, Yan

AU - Chen, Haitian

AU - Cote, Richard J

AU - Thompson, Mark E.

AU - Datar, Ram

AU - Zhou, Chongwu

PY - 2015/3/11

Y1 - 2015/3/11

N2 - Nanostructure field-effect transistor (FET) biosensors have shown great promise for ultra sensitive biomolecular detection. Top-down assembly of these sensors increases scalability and device uniformity but faces fabrication challenges in achieving the small dimensions needed for sensitivity. We report top-down fabricated indium oxide (In2O3) nanoribbon FET biosensors using highly scalable radio frequency (RF) sputtering to create uniform channel thicknesses ranging from 50 to 10 nm. We combine this scalable sensing platform with amplification from electronic enzyme-linked immunosorbent assay (ELISA) to achieve high sensitivity to target analytes such as streptavidin and human immunodeficiency virus type 1 (HIV-1) p24 proteins. Our approach circumvents Debye screening in ionic solutions and detects p24 protein at 20 fg/mL (about 250 viruses/mL or about 3 orders of magnitude lower than commercial ELISA) with a 35% conduction change in human serum. The In2O3 nanoribbon biosensors have 100% device yield and use a simple 2 mask photolithography process. The electrical properties of 50 In2O3 nanoribbon FETs showed good uniformity in on-state current, on/off current ratio, mobility, and threshold voltage. In addition, the sensors show excellent pH sensitivity over a broad range (pH 4 to 9) as well as over the physiological-related pH range (pH 6.8 to 8.2). With the demonstrated sensitivity, scalability, and uniformity, the In2O3 nanoribbon sensor platform makes great progress toward clinical testing, such as for early diagnosis of acquired immunodeficiency syndrome (AIDS).

AB - Nanostructure field-effect transistor (FET) biosensors have shown great promise for ultra sensitive biomolecular detection. Top-down assembly of these sensors increases scalability and device uniformity but faces fabrication challenges in achieving the small dimensions needed for sensitivity. We report top-down fabricated indium oxide (In2O3) nanoribbon FET biosensors using highly scalable radio frequency (RF) sputtering to create uniform channel thicknesses ranging from 50 to 10 nm. We combine this scalable sensing platform with amplification from electronic enzyme-linked immunosorbent assay (ELISA) to achieve high sensitivity to target analytes such as streptavidin and human immunodeficiency virus type 1 (HIV-1) p24 proteins. Our approach circumvents Debye screening in ionic solutions and detects p24 protein at 20 fg/mL (about 250 viruses/mL or about 3 orders of magnitude lower than commercial ELISA) with a 35% conduction change in human serum. The In2O3 nanoribbon biosensors have 100% device yield and use a simple 2 mask photolithography process. The electrical properties of 50 In2O3 nanoribbon FETs showed good uniformity in on-state current, on/off current ratio, mobility, and threshold voltage. In addition, the sensors show excellent pH sensitivity over a broad range (pH 4 to 9) as well as over the physiological-related pH range (pH 6.8 to 8.2). With the demonstrated sensitivity, scalability, and uniformity, the In2O3 nanoribbon sensor platform makes great progress toward clinical testing, such as for early diagnosis of acquired immunodeficiency syndrome (AIDS).

KW - biosensor

KW - electronic ELISA

KW - indium oxide

KW - nanoribbon

KW - top-down fabrication

UR - http://www.scopus.com/inward/record.url?scp=84924567246&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84924567246&partnerID=8YFLogxK

U2 - 10.1021/nl5047889

DO - 10.1021/nl5047889

M3 - Article

C2 - 25636984

AN - SCOPUS:84924567246

VL - 15

SP - 1943

EP - 1951

JO - Nano Letters

JF - Nano Letters

SN - 1530-6984

IS - 3

ER -