Highly differentiated fluorescence quenching of hemoglobin using a stilbazolium dye

Hye Jin Zo, James N Wilson, Jong S. Park

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

In this article, we reported here an effective and selective sensing of hemoglobin (Hb) with a stilbazolium dye, HNEP+. The nature of the HNEP+/Hb interaction was examined using a combination of various spectroscopic techniques. Complexation induced selective fluorescence quenching with a large Stern-Volmer constant, Ksv, the origin of which is attributed to the neutral isoelectric point (pI) of the proteins. The stoichiometry of the DNEP+:Hb was determined to be 1:2 by a Job's plot analysis, and the induced circular dichroism measurements revealed a loss of the α-helix and the unfolding of the proteins in the complex. The addition of cyclodextrin extended fluorescence lifetime, which ensured the accuracy and repeatability of the measurements. Finally, by taking advantage of a subtle change in pI and subsequently Ksv of the glycated Hb, HbA1c, we suggested the possibility of a new sensing concept for HbA1c. The current investigation demonstrates that a selective optical response of a stilbazolium dye can find significant usefulness in bio-sensing applications.

Original languageEnglish
Pages (from-to)38-42
Number of pages5
JournalDyes and Pigments
Volume101
DOIs
StatePublished - Jan 1 2014

Fingerprint

Hemoglobin
Quenching
Hemoglobins
Coloring Agents
Dyes
Fluorescence
Glycosylated Hemoglobin A
Cyclodextrins
Complexation
Proteins
Stoichiometry
Dichroism

Keywords

  • Chemosensors
  • Fluorescence quenching
  • Hemoglobin
  • Protein sensing
  • Stern-Volmer equation
  • Stilbazolium dye

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Process Chemistry and Technology

Cite this

Highly differentiated fluorescence quenching of hemoglobin using a stilbazolium dye. / Zo, Hye Jin; Wilson, James N; Park, Jong S.

In: Dyes and Pigments, Vol. 101, 01.01.2014, p. 38-42.

Research output: Contribution to journalArticle

@article{bb8af410e294432faa92c73586d73bbc,
title = "Highly differentiated fluorescence quenching of hemoglobin using a stilbazolium dye",
abstract = "In this article, we reported here an effective and selective sensing of hemoglobin (Hb) with a stilbazolium dye, HNEP+. The nature of the HNEP+/Hb interaction was examined using a combination of various spectroscopic techniques. Complexation induced selective fluorescence quenching with a large Stern-Volmer constant, Ksv, the origin of which is attributed to the neutral isoelectric point (pI) of the proteins. The stoichiometry of the DNEP+:Hb was determined to be 1:2 by a Job's plot analysis, and the induced circular dichroism measurements revealed a loss of the α-helix and the unfolding of the proteins in the complex. The addition of cyclodextrin extended fluorescence lifetime, which ensured the accuracy and repeatability of the measurements. Finally, by taking advantage of a subtle change in pI and subsequently Ksv of the glycated Hb, HbA1c, we suggested the possibility of a new sensing concept for HbA1c. The current investigation demonstrates that a selective optical response of a stilbazolium dye can find significant usefulness in bio-sensing applications.",
keywords = "Chemosensors, Fluorescence quenching, Hemoglobin, Protein sensing, Stern-Volmer equation, Stilbazolium dye",
author = "Zo, {Hye Jin} and Wilson, {James N} and Park, {Jong S.}",
year = "2014",
month = "1",
day = "1",
doi = "10.1016/j.dyepig.2013.09.027",
language = "English",
volume = "101",
pages = "38--42",
journal = "Dyes and Pigments",
issn = "0143-7208",
publisher = "Elsevier BV",

}

TY - JOUR

T1 - Highly differentiated fluorescence quenching of hemoglobin using a stilbazolium dye

AU - Zo, Hye Jin

AU - Wilson, James N

AU - Park, Jong S.

PY - 2014/1/1

Y1 - 2014/1/1

N2 - In this article, we reported here an effective and selective sensing of hemoglobin (Hb) with a stilbazolium dye, HNEP+. The nature of the HNEP+/Hb interaction was examined using a combination of various spectroscopic techniques. Complexation induced selective fluorescence quenching with a large Stern-Volmer constant, Ksv, the origin of which is attributed to the neutral isoelectric point (pI) of the proteins. The stoichiometry of the DNEP+:Hb was determined to be 1:2 by a Job's plot analysis, and the induced circular dichroism measurements revealed a loss of the α-helix and the unfolding of the proteins in the complex. The addition of cyclodextrin extended fluorescence lifetime, which ensured the accuracy and repeatability of the measurements. Finally, by taking advantage of a subtle change in pI and subsequently Ksv of the glycated Hb, HbA1c, we suggested the possibility of a new sensing concept for HbA1c. The current investigation demonstrates that a selective optical response of a stilbazolium dye can find significant usefulness in bio-sensing applications.

AB - In this article, we reported here an effective and selective sensing of hemoglobin (Hb) with a stilbazolium dye, HNEP+. The nature of the HNEP+/Hb interaction was examined using a combination of various spectroscopic techniques. Complexation induced selective fluorescence quenching with a large Stern-Volmer constant, Ksv, the origin of which is attributed to the neutral isoelectric point (pI) of the proteins. The stoichiometry of the DNEP+:Hb was determined to be 1:2 by a Job's plot analysis, and the induced circular dichroism measurements revealed a loss of the α-helix and the unfolding of the proteins in the complex. The addition of cyclodextrin extended fluorescence lifetime, which ensured the accuracy and repeatability of the measurements. Finally, by taking advantage of a subtle change in pI and subsequently Ksv of the glycated Hb, HbA1c, we suggested the possibility of a new sensing concept for HbA1c. The current investigation demonstrates that a selective optical response of a stilbazolium dye can find significant usefulness in bio-sensing applications.

KW - Chemosensors

KW - Fluorescence quenching

KW - Hemoglobin

KW - Protein sensing

KW - Stern-Volmer equation

KW - Stilbazolium dye

UR - http://www.scopus.com/inward/record.url?scp=84885344254&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84885344254&partnerID=8YFLogxK

U2 - 10.1016/j.dyepig.2013.09.027

DO - 10.1016/j.dyepig.2013.09.027

M3 - Article

AN - SCOPUS:84885344254

VL - 101

SP - 38

EP - 42

JO - Dyes and Pigments

JF - Dyes and Pigments

SN - 0143-7208

ER -