Higher PIK3C2B gene expression of H1N1+ specific B-cells is associated with lower H1N1 immunogenicity after trivalent influenza vaccination in HIV infected children

Nicola Cotugno, Sonia Zicari, Elena Morrocchi, Lesley R. de Armas, Suresh Pallikkuth, Stefano Rinaldi, Alessandra Ruggiero, Emma Concetta Manno, Paola Zangari, Maria Chiriaco, Stefania Bernardi, Sarah F. Andrews, Alberto Cagigi, Paolo Rossi, Adrian B. McDermott, Savita Pahwa, Paolo Palma

Research output: Contribution to journalArticle

Abstract

Perinatally HIV-infected children (PHIV), despite successful antiretroviral therapy, present suboptimal responses to vaccinations compared to healthy-controls (HC). Here we investigated phenotypic and transcriptional signatures of H1N1-specific B-cells (H1N1-Sp) in PHIV, differentially responding to trivalent-influenza-vaccine (TIV), and HC. Patients were categorized in responders (R) and non-responders (NR) according to hemagglutination-inhibition-assay at baseline and 21 days after TIV. No differences in H1N1-Sp frequencies were found between groups. H1N1-Sp transcriptional analysis revealed a distinct signature between PHIV and HC. NR presented higher PIK3C2B and NOD2 expression compared to R, confirmed by downregulation of PIK3C2B in resting-memory of R after H1N1 in-vitro stimulation. In conclusion this study confirms that qualitative rather than quantitative analyses are needed to characterize immune responses in PHIV. These results further suggest that higher PIK3C2B in H1N1-Sp of NR is associated with lower H1N1 immunogenicity and may be targeted by future modulating strategies to improve TIV responses in PHIV.

Original languageEnglish (US)
Article number108440
JournalClinical Immunology
Volume215
DOIs
StatePublished - Jun 2020

Keywords

  • Antigen specific B cells
  • Gene expression
  • H1N1
  • Influenza vaccination
  • PIK3C2B
  • Perinatally HIV infected children

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Cotugno, N., Zicari, S., Morrocchi, E., de Armas, L. R., Pallikkuth, S., Rinaldi, S., Ruggiero, A., Manno, E. C., Zangari, P., Chiriaco, M., Bernardi, S., Andrews, S. F., Cagigi, A., Rossi, P., McDermott, A. B., Pahwa, S., & Palma, P. (2020). Higher PIK3C2B gene expression of H1N1+ specific B-cells is associated with lower H1N1 immunogenicity after trivalent influenza vaccination in HIV infected children. Clinical Immunology, 215, [108440]. https://doi.org/10.1016/j.clim.2020.108440